Gas-Phase Helical Peptides Mimic Solution-Phase Behavior

2014 ◽  
Vol 136 (40) ◽  
pp. 14173-14183 ◽  
Author(s):  
Lindsay J. Morrison ◽  
Vicki H. Wysocki
Keyword(s):  
Phase Behavior ◽  
Gas Phase ◽  
Solution Phase ◽  
Helical Peptides

Gas-phase behavior of noncovalent transmembrane segment complexes

2008 ◽  
Vol 22 (24) ◽  
pp. 4089-4097 ◽  
Author(s):  
Linda M. M. Weigang ◽  
Dieter Langosch ◽  
Thomas Letzel
Keyword(s):  
Phase Behavior ◽  
Gas Phase ◽  
Transmembrane Segment

The Assessment of pH-Induced Supersaturation and Impact of an Additional Drug on the Solution Phase Behavior of Saquinavir

2018 ◽  
Vol 14 (4) ◽  
pp. 305-315 ◽  
Author(s):  
Sujata D. Sakhare ◽  
V. Sai Krishna Anand ◽  
Aishwarya Karan ◽  
K. Navya Sree ◽  
S. G. Vasantharaju ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Solution Phase

scholarly journals Liquid-gas phase behavior of Stockmayer fluid with high dipolar moment

2007 ◽  
Vol 10 (4) ◽  
pp. 553 ◽  
Author(s):  
Kalyuzhnyi ◽  
Protsykevitch ◽  
Cummings
Keyword(s):  
Phase Behavior ◽  
Gas Phase ◽  
Dipolar Moment

scholarly journals Bridging the Gap between the Gas Phase and Solution Phase: Solvent Specific Photochemistry in 4-tert-Butylcatechol

2015 ◽  
Vol 119 (50) ◽  
pp. 11989-11996 ◽  
Author(s):  
Michael D. Horbury ◽  
Lewis A. Baker ◽  
Wen-Dong Quan ◽  
Jamie D. Young ◽  
Michael Staniforth ◽  
...  
Keyword(s):  
Gas Phase ◽  
Solution Phase

scholarly journals Influence of membrane-spanning alpha-helical peptides on the phase behavior of the dioleoylphosphatidylcholine/water system

1997 ◽  
Vol 73 (6) ◽  
pp. 3078-3088 ◽  
Author(s):  
S. Morein ◽  
E. Strandberg ◽  
J.A. Killian ◽  
S. Persson ◽  
G. Arvidson ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Water System ◽  
Helical Peptides ◽  
Membrane Spanning ◽  
Alpha Helical Peptides

scholarly journals Photoproducts of the Photodynamic Therapy Agent Verteporfin Identified via Laser Interfaced Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5280
Author(s):  
Chris Furlan ◽  
Jacob A. Berenbeim ◽  
Caroline E. H. Dessent
Keyword(s):  
Mass Spectrometry ◽  
Photodynamic Therapy ◽  
Macular Degeneration ◽  
Gas Phase ◽  
Mass Spectrometer ◽  
Red Light ◽  
Solution Phase ◽  
Collision Induced Dissociation ◽  
Free Base ◽  
New Information

Verteporfin, a free base benzoporphyrin derivative monoacid ring A, is a photosensitizing drug for photodynamic therapy (PDT) used in the treatment of the wet form of macular degeneration and activated by red light of 689 nm. Here, we present the first direct study of its photofragmentation channels in the gas phase, conducted using a laser interfaced mass spectrometer across a broad photoexcitation range from 250 to 790 nm. The photofragmentation channels are compared with the collision-induced dissociation (CID) products revealing similar dissociation pathways characterized by the loss of the carboxyl and ester groups. Complementary solution-phase photolysis experiments indicate that photobleaching occurs in verteporfin in acetonitrile; a notable conclusion, as photoinduced activity in Verteporfin was not thought to occur in homogenous solvent conditions. These results provide unique new information on the thermal break-down products and photoproducts of this light-triggered drug.

Do electrospray mass spectra reflect the ligand binding state of proteins in solution?

2005 ◽  
Vol 83 (11) ◽  
pp. 1953-1960 ◽  
Author(s):  
Belal M Hossain ◽  
Douglas A Simmons ◽  
Lars Konermann
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Protein Interactions ◽  
Noncovalent Complex ◽  
Deuterium Exchange ◽  
Solution Phase ◽  
Hydrogen Deuterium Exchange ◽  
Esi Ms ◽  
Protein Ions ◽  
Hydrogen Deuterium

Electrospray ionization (ESI) mass spectrometry (MS) has become a popular tool for monitoring ligand–protein and protein–protein interactions. Due to the "gentle" nature of the ionization process, it is often possible to transfer weakly bound complexes into the gas phase, thus making them amenable to MS detection. One problem with this technique is the potential occurrence of fragmentation events during ESI. Also, some analytes tend to cluster together during ionization, thus forming nonspecific gas-phase assemblies that do not represent solution-phase complexes. In this work, we implemented a hydrogen–deuterium exchange (HDX) approach that can reveal whether or not the free and (or) bound constituents of a complex observed in ESI-MS reflect the binding situation in solution. Proteins are subjected to ESI immediately following an isotopic labeling pulse; only ligand-free and ligand-bound protein ions that were formed directly from the corresponding solution-phase species showed different HDX levels. Using myoglobin as a model system, it is demonstrated that this approach can readily distinguish scenarios where the heme–protein interactions were disrupted in solution from those where dissociation of the complex occurred in the gas phase. Experiments on cytochrome c strongly suggest that dimeric protein ions observed in ESI-MS reflect aggregates that were formed in solution.Key words: electrospray mass spectrometry, ligand–protein interaction, noncovalent complex, hydrogen–deuterium exchange, protein folding.

Sign in / Sign up

Export Citation Format

Share Document