Exploring the Formation and Recognition of an Important G-Quadruplex in a HIF1α Promoter and Its Transcriptional Inhibition by a Benzo[c]phenanthridine Derivative

2014 ◽  
Vol 136 (6) ◽  
pp. 2583-2591 ◽  
Author(s):  
Han Chen ◽  
Haitao Long ◽  
Xiaojie Cui ◽  
Jiang Zhou ◽  
Ming Xu ◽  
...  
Keyword(s):  
Transcriptional Inhibition ◽  
G Quadruplex ◽  
Phenanthridine Derivative

scholarly journals Promoter G-quadruplex folding precedes transcription and is controlled by chromatin

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jiazhen Shen ◽  
Dhaval Varshney ◽  
Angela Simeone ◽  
Xiaoyun Zhang ◽  
Santosh Adhikari ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Chromatin Accessibility ◽  
Pol Ii ◽  
Chromatin Compaction ◽  
Transcriptional Inhibition ◽  
G Quadruplex ◽  
Active Transcription ◽  
Pre Treatment ◽  
Dna Secondary Structures ◽  
The Relationship

Abstract Background Four-stranded G-quadruplexes (G4s) are DNA secondary structures in the human genome that are primarily found in active promoters associated with elevated transcription. Here, we explore the relationship between the folding of promoter G4s, transcription and chromatin state. Results Transcriptional inhibition by DRB or by triptolide reveals that promoter G4 formation, as assessed by G4 ChIP-seq, does not depend on transcriptional activity. We then show that chromatin compaction can lead to loss of promoter G4s and is accompanied by a corresponding loss of RNA polymerase II (Pol II), thus establishing a link between G4 formation and chromatin accessibility. Furthermore, pre-treatment of cells with a G4-stabilising ligand mitigates the loss of Pol II at promoters induced by chromatin compaction. Conclusions Overall, our findings show that G4 folding is coupled to the establishment of accessible chromatin and does not require active transcription.

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