Model Peptides Uncover the Role of the β-Secretase Transmembrane Sequence in Metal Ion Mediated Oligomerization

2013 ◽  
Vol 135 (51) ◽  
pp. 19354-19361 ◽  
Author(s):  
Lisa M. Munter ◽  
Holger Sieg ◽  
Tobias Bethge ◽  
Filip Liebsch ◽  
Frank S. Bierkandt ◽  
...  
Keyword(s):  
Metal Ion ◽  
Transmembrane Sequence ◽  
Model Peptides

scholarly journals Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium

2021 ◽  
Author(s):  
Stephanie Probst ◽  
Johannes Fels ◽  
Bettina Scharner ◽  
Natascha A. Wolff ◽  
Eleni Roussa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Cell Fate ◽  
Catalase Activity ◽  
Metal Ion ◽  
Iron Homeostasis ◽  
Collecting Duct ◽  
Duct Cell ◽  
Plasmid Transfection

AbstractThe liver hormone hepcidin regulates systemic iron homeostasis. Hepcidin is also expressed by the kidney, but exclusively in distal nephron segments. Several studies suggest hepcidin protects against kidney damage involving Fe2+ overload. The nephrotoxic non-essential metal ion Cd2+ can displace Fe2+ from cellular biomolecules, causing oxidative stress and cell death. The role of hepcidin in Fe2+ and Cd2+ toxicity was assessed in mouse renal cortical [mCCD(cl.1)] and inner medullary [mIMCD3] collecting duct cell lines. Cells were exposed to equipotent Cd2+ (0.5–5 μmol/l) and/or Fe2+ (50–100 μmol/l) for 4–24 h. Hepcidin (Hamp1) was transiently silenced by RNAi or overexpressed by plasmid transfection. Hepcidin or catalase expression were evaluated by RT-PCR, qPCR, immunoblotting or immunofluorescence microscopy, and cell fate by MTT, apoptosis and necrosis assays. Reactive oxygen species (ROS) were detected using CellROX™ Green and catalase activity by fluorometry. Hepcidin upregulation protected against Fe2+-induced mIMCD3 cell death by increasing catalase activity and reducing ROS, but exacerbated Cd2+-induced catalase dysfunction, increasing ROS and cell death. Opposite effects were observed with Hamp1 siRNA. Similar to Hamp1 silencing, increased intracellular Fe2+ prevented Cd2+ damage, ROS formation and catalase disruption whereas chelation of intracellular Fe2+ with desferrioxamine augmented Cd2+ damage, corresponding to hepcidin upregulation. Comparable effects were observed in mCCD(cl.1) cells, indicating equivalent functions of renal hepcidin in different collecting duct segments. In conclusion, hepcidin likely binds Fe2+, but not Cd2+. Because Fe2+ and Cd2+ compete for functional binding sites in proteins, hepcidin affects their free metal ion pools and differentially impacts downstream processes and cell fate.

scholarly journals Catalytic and structural role of the metal ion in dUTP pyrophosphatase

2003 ◽  
Vol 100 (10) ◽  
pp. 5670-5675 ◽  
Author(s):  
D. Mustafi ◽  
A. Bekesi ◽  
B. G. Vertessy ◽  
M. W. Makinen
Keyword(s):  
Metal Ion ◽  
Structural Role

Thermally Induced Electron Transfer in a CsCoFe Prussian Blue Derivative: The Specific Role of the Alkali-Metal Ion

2004 ◽  
Vol 116 (28) ◽  
pp. 3814-3817 ◽  
Author(s):  
Anne Bleuzen ◽  
Virginie Escax ◽  
Alban Ferrier ◽  
Françoise Villain ◽  
Michel Verdaguer ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Alkali Metal ◽  
Prussian Blue ◽  
Metal Ion ◽  
Specific Role ◽  
Alkali Metal Ion ◽  
Thermally Induced

Why do zeolites induce an unprecedented electronic state on exchanged metal ions?

2017 ◽  
Vol 19 (36) ◽  
pp. 25105-25114 ◽  
Author(s):  
Akira Oda ◽  
Takahiro Ohkubo ◽  
Takashi Yumura ◽  
Hisayoshi Kobayashi ◽  
Yasushige Kuroda
Keyword(s):  
Metal Ions ◽  
Electronic State ◽  
Base Metal ◽  
Metal Ion ◽  
High Efficiency ◽  
Lewis Base ◽  
Mfi Zeolite ◽  
Adsorption Processes ◽  
Exact Position

Understanding the exact position and the detailed role of the Al array in zeolites is essential for elucidating the origin of unique properties and for designing zeolite materials with high efficiency in catalytic and adsorption processes. In this work, we advanced pivotal roles of Lewis base–metal ion bifunctionality caused by Al atoms arrayed circumferentially in the MFI-zeolite pores.

One-dimensional cadmium(II)/bicinate(−1) complexes : The role of the alkali metal ion used in the reaction medium

Polyhedron ◽  
2011 ◽  
Vol 30 (2) ◽  
pp. 397-404 ◽  
Author(s):  
Eugenia Katsoulakou ◽  
Konstantis F. Konidaris ◽  
Aris Terzis ◽  
Catherine P. Raptopoulou ◽  
Spyros P. Perlepes ◽  
...  
Keyword(s):  
Alkali Metal ◽  
Metal Ion ◽  
Reaction Medium ◽  
Alkali Metal Ion ◽  
One Dimensional

scholarly journals Reduction by a Model of NAD(P)H. XIV. Mechanistic Consideration on the Role of Metal Ion

1977 ◽  
Vol 50 (6) ◽  
pp. 1535-1538 ◽  
Author(s):  
Atsuyoshi Ohno ◽  
Takahide Kimura ◽  
Hiroyuki Yamamoto ◽  
Seung Geon Kim ◽  
Shinzaburo Oka ◽  
...  
Keyword(s):  
Metal Ion

scholarly journals The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Yulia Sokurenko ◽  
Vera Ulyanova ◽  
Pavel Zelenikhin ◽  
Alexey Kolpakov ◽  
Dmitriy Blokhin ◽  
...  
Keyword(s):  
Metal Ions ◽  
Rna Structure ◽  
Metal Ion ◽  
Extracellular Enzymes ◽  
Bacillus Pumilus ◽  
Transition Metal Ions ◽  
The Body ◽  
Intestinal Microbiome ◽  
Phosphodiester Bond

Extracellular enzymes of intestinal microbiota are the key agents that affect functional activity of the body as they directly interact with epithelial and immune cells. Several species of theBacillusgenus, likeBacillus pumilus, a common producer of extracellular RNase binase, can populate the intestinal microbiome as a colonizing organism. Without involving metal ions as cofactors, binase depolymerizes RNA by cleaving the 3′,5′-phosphodiester bond and generates 2′,3′-cyclic guanosine phosphates in the first stage of a catalytic reaction. Maintained in the reaction mixture for more than one hour, such messengers can affect the human intestinal microflora and the human body. In the present study, we found that the rate of 2′,3′-cGMP was growing in the presence of transition metals that stabilized the RNA structure. At the same time, transition metal ions only marginally reduced the amount of 2′,3′-cGMP, blocking binase recognition sites of guanine at N7 of nucleophilic purine bases.

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