Benzimidazole-Based Ratiometric Two-Photon Fluorescent Probes for Acidic pH in Live Cells and Tissues

2013 ◽  
Vol 135 (47) ◽  
pp. 17969-17977 ◽  
Author(s):  
Hyung Joong Kim ◽  
Cheol Ho Heo ◽  
Hwan Myung Kim
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Acidic Ph ◽  
Two Photon

Lysosome-targeted two-photon fluorescent probes for rapid detection of H2S in live cells

2019 ◽  
Vol 43 (3) ◽  
pp. 1267-1274 ◽  
Author(s):  
Gongchun Li ◽  
Saige Ma ◽  
Jun Tang ◽  
Yong Ye
Keyword(s):  
Fluorescent Probes ◽  
Rapid Detection ◽  
High Sensitivity ◽  
Live Cells ◽  
Two Photon

Two novel two-photon fluorescent probes (BHNP-DA and M2) were designed and synthesized and show good selectivity and high sensitivity to H2S.

Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic pH-activatable fluorescent probe

2017 ◽  
Vol 53 (48) ◽  
pp. 6520-6523 ◽  
Author(s):  
Xilei Xie ◽  
Fuyan Tang ◽  
Xiaoyan Shangguan ◽  
Shiyi Che ◽  
Jinye Niu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Systems ◽  
Live Cells ◽  
Acidic Ph ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

A two-photon fluorescent probe presented lysosomal targetability and an acidic pH-activatable response toward formaldehyde, and was applied to visualize formaldehyde in living systems.

Dual-color imaging of cytosolic and mitochondrial zinc ions in live tissues with two-photon fluorescent probes

2014 ◽  
Vol 12 (21) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kailash Rathore ◽  
Chang Su Lim ◽  
Young Lee ◽  
Bong Rae Cho
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color ◽  
Living Tissues

We have developed TP probes for [Zn2+]cyto and [Zn2+]mito, which emit TPEF at widely-separated wavelength regions. The new probes can simultaneously detect [Zn2+]cyto and [Zn2+]mito in live cells, as well as in living tissues by dual-color TPM imaging.

scholarly journals A synergistic strategy to develop photostable and bright dyes with long Stokes shift for super-resolution microscopy

2021 ◽  
Author(s):  
gangwei jiang ◽  
Tian-Bing Ren ◽  
Elisa D’Este ◽  
mengyi xiong ◽  
Bin Xiong ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Stokes Shift ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Live Cells ◽  
Sted Microscopy ◽  
New Class ◽  
Two Photon ◽  
Cellular Environment ◽  
Super Resolution Microscopy

Abstract The quality and application of super-resolution fluorescence imaging greatly lie in the properties of fluorescent probes. However, conventional fluorophores in a cellular environment often suffer from low brightness, poor photostability, and short Stokes shift (< 30 nm). Here we report a synergistic strategy to simultaneously improve such properties of regular fluorophores. Introduction of quinoxaline motif with fine-tuned electron density to conventional rhodamines generates new dyes with vibronic structure and inhibited twisted-intramolecular-charge-transfer (TICT) formation synchronously, thus increasing the brightness and photostability as well as Stokes shift. The new fluorophore BDQF-6 exhibits around twofold greater brightness (ε × Φ = 6.6 × 104 L·mol− 1·cm− 1) and Stokes shift (56 nm) than its parental fluorophore, Rhodamine B. Importantly, in Stimulated Emission Depletion (STED) microscopy, BDQF-6 derived probe possesses a superior photostability and thus renders threefold more frames than carbopyronine- and JF608-based probes, known as photostable fluorophores for STED imaging. More BDQF-6 derivatives were developed next, allowing us to perform wash-free organelles (mitochondria and lysosome) staining and protein labeling with ultrahigh signal-to-noise ratios (up to 106 folds) in confocal and STED microscopy of live cells, or two-photon and 3D STED microscopy of fixed cells. Furthermore, the strategy was well generalized to different types of dyes (pyronin, rhodol, coumarin, and Boranil), offering a new class of bright and photostable fluorescent probes with long Stokes shift (up to 136 nm) for bioimaging and biosensing.

Two-Photon Fluorescent Probes for Acidic Vesicles in Live Cells and Tissue

2008 ◽  
Vol 120 (12) ◽  
pp. 2263-2266 ◽  
Author(s):  
Hwan Myung Kim ◽  
Myoung Jin An ◽  
Jin Hee Hong ◽  
Byeong Ha Jeong ◽  
Ohyun Kwon ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Two Photon ◽  
Acidic Vesicles

Targetable, two-photon fluorescent probes for local nitric oxide capture in the plasma membranes of live cells and brain tissues

The Analyst ◽  
2018 ◽  
Vol 143 (17) ◽  
pp. 4180-4188 ◽  
Author(s):  
Xinfu Zhang ◽  
Benlei Wang ◽  
Yi Xiao ◽  
Chao Wang ◽  
Ling He
Keyword(s):  
Nitric Oxide ◽  
Plasma Membrane ◽  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Plasma Membranes ◽  
Live Cells ◽  
Two Photon ◽  
In Cells ◽  
Brain Tissues

A plasma membrane-targetable two-photon fluorescent probe for capturing nitric oxide in cells and brain tissues.

scholarly journals Semi-Quantitatively Designing Two-Photon High-Performance Fluorescent Probes for Glutathione S-Transferases

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Xue-Xiang Zhang ◽  
Huan Qi ◽  
Mei-Heng Lu ◽  
Song-Qiu Yang ◽  
Peng Li ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
High Performance ◽  
Density Functional ◽  
Transient Absorption ◽  
Theoretical Calculations ◽  
Detoxification Enzymes ◽  
Live Cells ◽  
Conceptual Density Functional Theory ◽  
Two Photon ◽  
Glutathione S Transferases

Glutathione S-transferases (GSTs), detoxification enzymes that catalyze the addition of glutathione (GSH) to diverse electrophilic molecules, are often overexpressed in various tumor cells. While fluorescent probes for GSTs have often adopted the 2,4-dinitrobenzenesulfonyl (DNs) group as the receptor unit, they usually suffer from considerable background reaction noise with GSH due to excessive electron deficiency. However, weakening this reactivity is generally accompanied by loss of sensitivity for GSTs, and therefore, finely turning down the reactivity while maintaining certain sensitivity is critical for developing a practical probe. Here, we report a rational semiquantitative strategy for designing such a practical two-photon probe by introducing a parameter adopted from the conceptual density functional theory (CDFT), the local electrophilicity ωk, to characterize this reactivity. As expected, kinetic studies established ωk as efficient to predict the reactivity with GSH, and probe NI3 showing the best performance was successfully applied to detecting GST activities in live cells and tissue sections with high sensitivity and signal-to-noise ratio. Photoinduced electron transfer of naphthalimide-based probes, captured by femtosecond transient absorption for the first time and unraveled by theoretical calculations, also contributes to the negligible background noise.

Two-Photon Fluorescent Probes for Acidic Vesicles in Live Cells and Tissue

2008 ◽  
Vol 47 (12) ◽  
pp. 2231-2234 ◽  
Author(s):  
Hwan Myung Kim ◽  
Myoung Jin An ◽  
Jin Hee Hong ◽  
Byeong Ha Jeong ◽  
Ohyun Kwon ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Two Photon ◽  
Acidic Vesicles

scholarly journals Fluorescent Probes for Live Cell Thiol Detection

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3575
Author(s):  
Shenggang Wang ◽  
Yue Huang ◽  
Xiangming Guan
Keyword(s):  
Fluorescent Probes ◽  
Biological System ◽  
High Sensitivity ◽  
Intracellular Distribution ◽  
Live Cell ◽  
Live Cells ◽  
High Demand ◽  
Non Invasive

Thiols play vital and irreplaceable roles in the biological system. Abnormality of thiol levels has been linked with various diseases and biological disorders. Thiols are known to distribute unevenly and change dynamically in the biological system. Methods that can determine thiols’ concentration and distribution in live cells are in high demand. In the last two decades, fluorescent probes have emerged as a powerful tool for achieving that goal for the simplicity, high sensitivity, and capability of visualizing the analytes in live cells in a non-invasive way. They also enable the determination of intracellular distribution and dynamitic movement of thiols in the intact native environments. This review focuses on some of the major strategies/mechanisms being used for detecting GSH, Cys/Hcy, and other thiols in live cells via fluorescent probes, and how they are applied at the cellular and subcellular levels. The sensing mechanisms (for GSH and Cys/Hcy) and bio-applications of the probes are illustrated followed by a summary of probes for selectively detecting cellular and subcellular thiols.

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