scholarly journals Nanoscale Metal–Organic Frameworks for the Co-Delivery of Cisplatin and Pooled siRNAs to Enhance Therapeutic Efficacy in Drug-Resistant Ovarian Cancer Cells

2014 ◽  
Vol 136 (14) ◽  
pp. 5181-5184 ◽  
Author(s):  
Chunbai He ◽  
Kuangda Lu ◽  
Demin Liu ◽  
Wenbin Lin
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Therapeutic Efficacy ◽  
Metal Organic Frameworks ◽  
Ovarian Cancer Cells ◽  
Drug Resistant ◽  
Metal Organic

Kuguacin J isolated from bitter melon leaves modulates paclitaxel sensitivity in drug-resistant human ovarian cancer cells

2017 ◽  
Vol 71 (4) ◽  
pp. 693-702 ◽  
Author(s):  
Pornsiri Pitchakarn ◽  
Sonthaya Umsumarng ◽  
Sariya Mapoung ◽  
Pisamai Ting ◽  
Piya Temviriyanukul ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Bitter Melon ◽  
Drug Resistant

scholarly journals Soft drug-resistant ovarian cancer cells migrate via two distinct mechanisms utilizing myosin II-based contractility

2018 ◽  
Vol 1865 (2) ◽  
pp. 392-405 ◽  
Author(s):  
Aastha Kapoor ◽  
Amlan Barai ◽  
Bhushan Thakur ◽  
Alakesh Das ◽  
Sejal R. Patwardhan ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Myosin Ii ◽  
Ovarian Cancer Cells ◽  
Drug Resistant ◽  
Soft Drug

scholarly journals Enzyme-Regulated Supramolecular Assemblies of Cholesterol Conjugates against Drug-Resistant Ovarian Cancer Cells

2016 ◽  
Vol 138 (34) ◽  
pp. 10758-10761 ◽  
Author(s):  
Huaimin Wang ◽  
Zhaoqianqi Feng ◽  
Dongdong Wu ◽  
Keith J. Fritzsching ◽  
Mike Rigney ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Supramolecular Assemblies ◽  
Ovarian Cancer Cells ◽  
Drug Resistant

scholarly journals RNAi-mediated knockdown of the CLN3 gene inhibits proliferation and promotes apoptosis in drug-resistant ovarian cancer cells

2015 ◽  
Vol 12 (5) ◽  
pp. 6635-6641 ◽  
Author(s):  
DONGWEI MAO ◽  
JIANHUA CHE ◽  
SHIYU HAN ◽  
HONGHUI ZHAO ◽  
YUMEI ZHU ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Drug Resistant

scholarly journals CPEB4-Promoted Paclitaxel Resistance in Ovarian Cancer In Vitro Relies on Translational Regulation of CSAG2

2021 ◽  
Vol 11 ◽  
Author(s):  
Yaqing Zhang ◽  
Hongyun Gan ◽  
Fei Zhao ◽  
Xiaomei Ma ◽  
Xiaofeng Xie ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Rna Binding ◽  
Ovarian Cancer Cells ◽  
Drug Resistant ◽  
Paclitaxel Resistance ◽  
Cytoplasmic Polyadenylation

Background: Drug resistance is a major obstacle in chemotherapy for ovarian cancer, wherein the up regulation of drug-resistant genes plays an important role. The cytoplasmic polyadenylation element binding protein 4 (CPEB4) is an RNA binding protein that controls mRNA cytoplasmic polyadenylation and translation.Methods: The expression of CPEB4 in paclitaxel-resistant ovarian cancer cell lines and recurrent ovarian tumors relative to counterparts was determined by qRT-PCR, Western blotting and immunohistochemistry. The response to paclitaxel treatment was evaluated by cellular viability test and colony formation assay. RNA immunoprecipitation and poly(A) tail test were applied to examine the levels of RNA binding and cytoplasmic polyadenylation.Results: CPEB4 is elevated in paclitaxel-resistant ovarian cancer cells and recurrent ovarian tumors treated with paclitaxel-based chemotherapy. In addition, CPEB4 overexpression promotes paclitaxel resistance in ovarian cancer cells in vitro, and vice versa, CPEB4 knockdown restores paclitaxel sensitivity, indicating that CPEB4 confers paclitaxel resistance in ovarian cancer cells. Mechanistically, CPEB4 binds with the taxol (paclitaxel)-resistance-associated gene-3 (TRAG-3/CSAG2) mRNAs and induces its expression at a translational level. Moreover, CSAG2 expression is upregulated in paclitaxel-resistant ovarian carcinoma and cancer cell lines, and more importantly, siRNA-mediated CSAG2 knockdown overtly attenuates CPEB4-mediated paclitaxel resistance.Conclusion: This study suggests that the drug-resistant protein CSAG2 is translationally induced by CPEB4, which underlies CPEB4-promoted paclitaxel resistance in ovarian cancer in vitro. Thus, interfering CPEB4/CSAG2 axis might be of benefit to overcome paclitaxel-resistant ovarian cancer.

scholarly journals EfficientIn VitroTRAIL-Gene Delivery in Drug-Resistant A2780/DDP Ovarian Cancer Cell Line via Magnetofection

2011 ◽  
Vol 2011 ◽  
pp. 1-7
Author(s):  
Fang Li ◽  
Sumei Niu ◽  
Jing Sun ◽  
Huaishi Zhu ◽  
Qiujie Ba ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Western Blot ◽  
Cancer Cells ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Gene Transfection ◽  
Ovarian Cancer Cells ◽  
Drug Resistant

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) presents great promise as an anticancer agent for human cancer therapy. In this study, a magnetofection agent (polyMAG-l000) was evaluated forin vitrodelivery of TRAIL gene towards drug-resistant A2780/DDP ovarian cancer cells. Transfection experiments showed that polyMAG-l000 was able to transfect A2780/DDP cellsin vitro, leading to a higher level of TRAIL gene expression in the presence of a static magnetic field as compared to other transfection agent, such as Lipofectamine 2000. TRAIL gene expression in the A2780/DDP cells was also confirmed by Western blot analysis. Moreover, the TRAIL gene expression exhibited remarkable decrease in the cell viability, as determined by MTT assay. Importantly, PolyMAG-l000-mediated TRAIL gene transfection in the presence of anticancer drug cisplatin (CDDP) induced much higher percentages of apoptotic A2780/DDP cells, compared to TRAIL gene transfection or CDDP treatment alone. A further study by Western blot analysis indicated that cytochromecrelease and caspase-9 cleavage pathway were associated with the initiation of the apoptosis in A2780/DDP cells. The results of this study indicate that polyMAG-l000 can be used as an efficient agent for TRAIL gene transfection in ovarian cancer cells.

scholarly journals Nano-mechanical Reinforcement in Drug-Resistant Ovarian Cancer Cells

2015 ◽  
Vol 38 (3) ◽  
pp. 389-395 ◽  
Author(s):  
Young Ho Seo ◽  
Yoo-na Jo ◽  
Yong Jin Oh ◽  
Soyeun Park
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Drug Resistant ◽  
Mechanical Reinforcement
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