scholarly journals Transforming Thymidine into a Magnetic Resonance Imaging Probe for Monitoring Gene Expression

2013 ◽  
Vol 135 (4) ◽  
pp. 1617-1624 ◽  
Author(s):  
Amnon Bar-Shir ◽  
Guanshu Liu ◽  
Yajie Liang ◽  
Nirbhay N. Yadav ◽  
Michael T. McMahon ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Gene Expression ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Imaging Probe ◽  
Monitoring Gene Expression

scholarly journals Detecting Chronic Post-Traumatic Osteomyelitis of Mouse Tibia via an IL-13Rα2 Targeted Metallofullerene Magnetic Resonance Imaging Probe

2017 ◽  
Vol 28 (2) ◽  
pp. 649-658 ◽  
Author(s):  
Li Xiao ◽  
Tinghui Li ◽  
Mengmeng Ding ◽  
Jiezuan Yang ◽  
José Rodríguez-Corrales ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Imaging Probe ◽  
Post Traumatic ◽  
Mouse Tibia

scholarly journals A Genetically Encoded Reporter for Diffusion Weighted Magnetic Resonance Imaging

2016 ◽  
Author(s):  
Arnab Mukherjee ◽  
Di Wu ◽  
Hunter C. Davis ◽  
Mikhail G. Shapiro
Keyword(s):  
Magnetic Resonance Imaging ◽  
Gene Expression ◽  
Magnetic Resonance ◽  
Chemical Exchange ◽  
Water Channel ◽  
Chemical Exchange Saturation Transfer ◽  
Diffusion Weighted Mri ◽  
Resonance Imaging ◽  
Diffusion Weighted ◽  
Water Diffusivity

The ability to monitor gene expression in intact, optically opaque animals is important for a multitude of applications including longitudinal imaging of transgene expression and long term tracking of cell based therapeutics. Magnetic resonance imaging (MRI) could enable such monitoring with high spatial and temporal resolution. However, existing MRI reporter genes, based primarily on metal-binding proteins or chemical exchange saturation transfer probes, are limited by their reliance on metal ions or relatively low sensitivity. In this work, we introduce a new class of genetically encoded reporters for MRI that work by altering water diffusivity. We show that overexpression of the human water channel aquaporin 1 (AQP1) produces robust contrast in diffusion weighted MRI by increasing effective water diffusivity in tissues by over 100% without affecting cell viability or morphology. Low levels of AQP1 expression (~1 μM), or mixed populations comprising as few as 10% AQP1-expressing cells, produce sufficient contrast to be observed by MRI. We demonstrate the utility of AQP1 in vivo by imaging gene expression in intracranial tumor xenografts. Overall, our results establish AQP1 as a new, metal-free, nontoxic and sensitive genetically encoded reporter for diffusion weighted MRI.

Sign in / Sign up

Export Citation Format

Share Document