Alkyne-Tag Raman Imaging for Visualization of Mobile Small Molecules in Live Cells

2012 ◽  
Vol 134 (51) ◽  
pp. 20681-20689 ◽  
Author(s):  
Hiroyuki Yamakoshi ◽  
Kosuke Dodo ◽  
Almar Palonpon ◽  
Jun Ando ◽  
Katsumasa Fujita ◽  
...  
Keyword(s):  
Small Molecules ◽  
Raman Imaging ◽  
Live Cells

Alkyne-tag Raman imaging of bio-active small molecules in live cells

2015 ◽  
Author(s):  
Jun Ando ◽  
Almar F. Palonpon ◽  
Hiroyuki Yamakoshi ◽  
Kosuke Dodo ◽  
Satoshi Kawata ◽  
...  
Keyword(s):  
Small Molecules ◽  
Raman Imaging ◽  
Live Cells

scholarly journals Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

2021 ◽  
Author(s):  
Syusuke Egoshi ◽  
Kosuke Dodo ◽  
Kenji Ohgane ◽  
Mikiko Sodeoka
Keyword(s):  
Small Molecules ◽  
Raman Imaging ◽  
Live Cell ◽  
Live Cells ◽  
Terminal Alkynes

Two-color Raman imaging of D-alkynes and H-alkynes makes it possible to distinguish and observe similar small molecules in live cells.

Watching bioactive small molecules in live cells by alkyne-tag Raman imaging

2013 ◽  
Author(s):  
Almar Palonpon ◽  
Hiroyuki Yamakoshi ◽  
Kosuke Dodo ◽  
Jun Ando ◽  
Satoshi Kawata ◽  
...  
Keyword(s):  
Small Molecules ◽  
Raman Imaging ◽  
Live Cells

scholarly journals CBMT-07. BIOMARKERS OF AGGRESSIVENESS IN IDH MUTANT GLIOMA

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi34-vi34
Author(s):  
Tomohiro Yamasaki ◽  
Adrian Lita ◽  
Tyrone Dowdy ◽  
Mark Gilbert ◽  
Mioara Larion
Keyword(s):  
Cell Lines ◽  
Imaging Spectroscopy ◽  
Mutant Gene ◽  
Raman Imaging ◽  
Malignant Progression ◽  
Mass Spectrometry Analysis ◽  
Live Cells ◽  
Lower Grade ◽  
Spectrometry Analysis ◽  
Idh Mutations

Abstract BACKGROUND Gliomas with isocitrate dehydrogenase (IDH) mutations in adults evolve from lower-grade gliomas to secondary glioblastomas (GBM), a fatal disease with fast progression. IDH mutation occurs early in tumorigenesis, and persistently contribute to the reprograming of glucose, lipid and amino acid metabolism. This offer a plethora of potential biomarkers of progression. However, because it is extremely difficult to detect the distribution and transfer of metabolites changing in every moment in a single cell, the involvement of metabolites produced by mutant IDH in malignant progression remains understudied. MATERIALS AND METHODS Raman imaging spectroscopy, which can image chemical bonds and concentration of molecules at submicron spatial resolution, enables detection of spatiotemporal changes of metabolomes in live cells. We developed the software called Biomolecular Component Analysis (BCAbox) to deconvolute the recorded raw Raman spectra, leading to detection of unique spectral features of different classes of biomolecules. RESULTS AND CONCLUSIONS We applied Raman imaging spectroscopy to GBM cell lines that were transfected with IDH1 mutant gene. Our results indicated that lipid metabolism has a unique profile in IDH1 mutant gliomas. Subsequent mass spectrometry analysis of extracted organelle revealed the exact classes of lipids altered in the IDH mutant glioma and suggested biomarkers unique to IDH1 mutant. We will report our validation studies of the biomarkers in patient-derived IDH mutant glioma cell lines and patients derived-orthotopic xenograft mouse models with different degrees of aggressiveness and in matched primary versus recurrent gliomas. The results of the present study may provide novel insights into the discovery of metabolic biomarkers for the malignant progression in IDH mutant gliomas.

scholarly journals Genomic targeting of epigenetic probes using a chemically tailored Cas9 system

2017 ◽  
Vol 114 (4) ◽  
pp. 681-686 ◽  
Author(s):  
Glen P. Liszczak ◽  
Zachary Z. Brown ◽  
Samuel H. Kim ◽  
Rob C. Oslund ◽  
Yael David ◽  
...  
Keyword(s):  
Dna Binding ◽  
Chemical Synthesis ◽  
Small Molecules ◽  
Small Molecule ◽  
Binding Sites ◽  
Dna Binding Protein ◽  
Dna Binding Proteins ◽  
Live Cells ◽  
Binding Partners

Recent advances in the field of programmable DNA-binding proteins have led to the development of facile methods for genomic localization of genetically encodable entities. Despite the extensive utility of these tools, locus-specific delivery of synthetic molecules remains limited by a lack of adequate technologies. Here we combine the flexibility of chemical synthesis with the specificity of a programmable DNA-binding protein by using protein trans-splicing to ligate synthetic elements to a nuclease-deficient Cas9 (dCas9) in vitro and subsequently deliver the dCas9 cargo to live cells. The versatility of this technology is demonstrated by delivering dCas9 fusions that include either the small-molecule bromodomain and extra-terminal family bromodomain inhibitor JQ1 or a peptide-based PRC1 chromodomain ligand, which are capable of recruiting endogenous copies of their cognate binding partners to targeted genomic binding sites. We expect that this technology will allow for the genomic localization of a wide array of small molecules and modified proteinaceous materials.

Photothermal release of small molecules from gold nanoparticles in live cells

2012 ◽  
Vol 8 (6) ◽  
pp. 908-915 ◽  
Author(s):  
Wesley F. Zandberg ◽  
Amir Bahman Samsam Bakhtiari ◽  
Zach Erno ◽  
Dennis Hsiao ◽  
Byron D. Gates ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecules ◽  
Live Cells

scholarly journals Integration of diffraction phase microscopy and Raman imaging for label‐free morpho‐molecular assessment of live cells

2018 ◽  
Vol 12 (4) ◽  
pp. e201800291 ◽  
Author(s):  
Rishikesh Pandey ◽  
Renjie Zhou ◽  
Rosalie Bordett ◽  
Ciera Hunter ◽  
Kristine Glunde ◽  
...  
Keyword(s):  
Raman Imaging ◽  
Live Cells ◽  
Label Free ◽  
Phase Microscopy ◽  
Molecular Assessment
Sign in / Sign up

Export Citation Format

Share Document