scholarly journals Spectroscopic Evidence for and Characterization of a Trinuclear Ferroxidase Center in Bacterial Ferritin from Desulfovibrio vulgaris Hildenborough

2012 ◽  
Vol 134 (26) ◽  
pp. 10822-10832 ◽  
Author(s):  
Alice S. Pereira ◽  
Cristina G. Timóteo ◽  
Márcia Guilherme ◽  
Filipe Folgosa ◽  
Sunil G. Naik ◽  
...  
Keyword(s):  
Desulfovibrio Vulgaris ◽  
Spectroscopic Evidence ◽  
Ferroxidase Center ◽  
Bacterial Ferritin ◽  
Desulfovibrio Vulgaris Hildenborough

Characterization of the [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough

2018 ◽  
pp. 169-201 ◽  
Author(s):  
Sónia Zacarias ◽  
Marisela Vélez ◽  
Marcos Pita ◽  
Antonio L. De Lacey ◽  
Pedro M. Matias ◽  
...  
Keyword(s):  
Desulfovibrio Vulgaris ◽  
Desulfovibrio Vulgaris Hildenborough

Functional characterization of the early steps of tetrapyrrole biosynthesis and modification in Desulfovibrio vulgaris Hildenborough

2009 ◽  
Vol 420 (2) ◽  
pp. 317-326 ◽  
Author(s):  
Susana A. L. Lobo ◽  
Amanda Brindley ◽  
Martin J. Warren ◽  
Lígia M. Saraiva
Keyword(s):  
Functional Characterization ◽  
Desulfovibrio Vulgaris ◽  
Decarboxylase Activity ◽  
Sulfate Reducing ◽  
Porphobilinogen Synthase ◽  
Desulfovibrio Vulgaris Hildenborough ◽  
The Individual ◽  
Individual Enzyme ◽  
Relevant Gene

The biosynthesis of the tetrapyrrole framework has been investigated in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough by characterization of the enzymes required for the transformation of aminolaevulinic acid into sirohydrochlorin. PBG (porphobilinogen) synthase (HemB) was found to be a zinc-dependent enzyme that exists in its native state as a homohexamer. PBG deaminase (HemC) was shown to contain the dipyrromethane cofactor. Uroporphyrinogen III synthase is found fused with a uroporphyrinogen III methyltransferase (HemD-CobA). Both activities could be demonstrated in this amalgamated protein and the individual enzyme activities were separated by dissecting the relevant gene to allow the production of two distinct proteins. A gene annotated in the genome as a bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase was in fact shown to act only as a dehydrogenase and is simply capable of synthesizing sirohydrochlorin rather than sirohaem. Genome analysis also reveals a lack of any uroporphyrinogen III decarboxylase, an enzyme necessary for the classical route to haem synthesis. However, the genome does encode some predicted haem d1 biosynthetic enzymes even though the bacterium does not contain the cd1 nitrite reductase. We suggest that sirohydrochlorin acts as a substrate for haem synthesis using a novel pathway that involves homologues of the d1 biogenesis system. This explains why the uroporphyrinogen III synthase is found fused with the methyltransferase, bypassing the need for uroporphyrinogen III decarboxylase activity.

Characterization of a Ferredoxin from Desulfovibrio Vulgaris (Hildenborough) that Interacts with RNA

1995 ◽  
Vol 231 (2) ◽  
pp. 352-357 ◽  
Author(s):  
Alexander F. Arendsen ◽  
Jos Schalk ◽  
Walter M. A. M. Dongen ◽  
Wilfred R. Hagen
Keyword(s):  
Desulfovibrio Vulgaris ◽  
Desulfovibrio Vulgaris Hildenborough

scholarly journals Characterization of a Ferredoxin from Desulfovibrio Vulgaris (Hildenborough) that Interacts with RNA

2008 ◽  
Vol 231 (2) ◽  
pp. 352-357
Author(s):  
Alexander F. Arendsen ◽  
Jos Schalk ◽  
Walter M. A. M. Dongen ◽  
Wilfred R. Hagen
Keyword(s):  
Desulfovibrio Vulgaris ◽  
Desulfovibrio Vulgaris Hildenborough

scholarly journals Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli

1987 ◽  
Vol 162 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Gerrit VOORDOUW ◽  
Wilfred R. HAGEN ◽  
K. Margreet KRUSE-WOLTERS ◽  
Anita BERKEL-ARTS ◽  
Cees VEEGER
Keyword(s):  
Escherichia Coli ◽  
Desulfovibrio Vulgaris ◽  
Purification And Characterization ◽  
Desulfovibrio Vulgaris Hildenborough

FTIR spectroelectrochemical characterization of the Ni–Fe–Se hydrogenase from Desulfovibrio vulgaris Hildenborough

2008 ◽  
Vol 13 (8) ◽  
pp. 1315-1320 ◽  
Author(s):  
Antonio L. De Lacey ◽  
Cristina Gutiérrez-Sánchez ◽  
Víctor M. Fernández ◽  
Isabel Pacheco ◽  
Inês A. C. Pereira
Keyword(s):  
Desulfovibrio Vulgaris ◽  
Desulfovibrio Vulgaris Hildenborough
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