scholarly journals Electron Tunneling Pathways and Role of Adenine in Repair of Cyclobutane Pyrimidine Dimer by DNA Photolyase

2012 ◽  
Vol 134 (19) ◽  
pp. 8104-8114 ◽  
Author(s):  
Zheyun Liu ◽  
Xunmin Guo ◽  
Chuang Tan ◽  
Jiang Li ◽  
Ya-Ting Kao ◽  
...  
Keyword(s):  
Electron Tunneling ◽  
Pyrimidine Dimer ◽  
Cyclobutane Pyrimidine Dimer ◽  
Dna Photolyase ◽  
Tunneling Pathways

scholarly journals The Roles of Several Residues ofEscherichia coliDNA Photolyase in the Highly Efficient Photo-Repair of Cyclobutane Pyrimidine Dimers

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Lei Xu ◽  
Guoping Zhu
Keyword(s):  
Escherichia Coli ◽  
Energy Source ◽  
Pyrimidine Dimer ◽  
Cyclobutane Pyrimidine Dimer ◽  
Model Compounds ◽  
Cyclobutane Pyrimidine Dimers ◽  
Dna Photolyase ◽  
Pyrimidine Dimers ◽  
Repair Efficiency ◽  
Very High

Escherichia coliDNA photolyase is an enzyme that repairs the major kind of UV-induced lesions, cyclobutane pyrimidine dimer (CPD) in DNA utilizing 350–450 nm light as energy source. The enzyme has very high photo-repair efficiency (the quantum yield of the reaction is ~0.85), which is significantly greater than many model compounds that mimic photolyase. This suggests that some residues of the protein play important roles in the photo-repair of CPD. In this paper, we have focused on several residues discussed their roles in catalysis by reviewing the existing literature and some hypotheses.

phrA, the major photoreactivating factor in the cyanobacterium Synechocystis sp. strain PCC 6803 codes for a cyclobutane-pyrimidine-dimer-specific DNA photolyase

2000 ◽  
Vol 173 (5-6) ◽  
pp. 412-417 ◽  
Author(s):  
Wing-On Ng ◽  
Rodolfo Zentella ◽  
Yinsheng Wang ◽  
John-Stephen A. Taylor ◽  
Himadri B. Pakrasi
Keyword(s):  
Pyrimidine Dimer ◽  
Cyclobutane Pyrimidine Dimer ◽  
Dna Photolyase ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Dynamics and mechanism of cyclobutane pyrimidine dimer repair by DNA photolyase

2011 ◽  
Vol 108 (36) ◽  
pp. 14831-14836 ◽  
Author(s):  
Z. Liu ◽  
C. Tan ◽  
X. Guo ◽  
Y.-T. Kao ◽  
J. Li ◽  
...  
Keyword(s):  
Pyrimidine Dimer ◽  
Cyclobutane Pyrimidine Dimer ◽  
Dna Photolyase

scholarly journals Genome-wide transcription-coupled repair inEscherichia coliis mediated by the Mfd translocase

2017 ◽  
Vol 114 (11) ◽  
pp. E2116-E2125 ◽  
Author(s):  
Ogun Adebali ◽  
Yi-Ying Chiou ◽  
Jinchuan Hu ◽  
Aziz Sancar ◽  
Christopher P. Selby
Keyword(s):  
High Throughput Sequencing ◽  
Excision Repair ◽  
Coupling Factor ◽  
Pyrimidine Dimer ◽  
Cyclobutane Pyrimidine Dimer ◽  
Predominant Role ◽  
Genome Wide Analysis ◽  
Genome Wide

We used high-throughput sequencing of short, cyclobutane pyrimidine dimer-containing ssDNA oligos generated during repair of UV-induced damage to study that process at both mechanistic and systemic levels inEscherichia coli. Numerous important insights on DNA repair were obtained, bringing clarity to the respective roles of UvrD helicase and Mfd translocase in repair of UV-induced damage. Mechanistically, experiments showed that the predominant role of UvrD in vivo is to unwind the excised 13-mer from dsDNA and that mutation ofuvrDresults in remarkable protection of that oligo from exonuclease activity as it remains hybridized to the dsDNA. Genome-wide analysis of the transcribed strand/nontranscribed strand (TS/NTS) repair ratio demonstrated that deletion ofmfdglobally shifts the distribution of TS/NTS ratios downward by a factor of about 2 on average for the most highly transcribed genes. Even for the least transcribed genes, Mfd played a role in preferential repair of the transcribed strand. On the other hand, mutation ofuvrD, if anything, slightly pushed the distribution of TS/NTS ratios to higher ratios. These results indicate that Mfd is the transcription repair-coupling factor whereas UvrD plays a role in excision repair by aiding the catalytic turnover of excision repair proteins.

scholarly journals A Computational Study on the Role of a Methionine at a Discriminator Site of Cyclobutane-Pyrimidine-Dimer Photolyase

2014 ◽  
Vol 106 (2) ◽  
pp. 690a
Author(s):  
Ryuma Sato ◽  
Hirotaka Kitoh-Nishioka ◽  
Tsutomu Kawatsu ◽  
Kei Yura ◽  
Koji Ando ◽  
...  
Keyword(s):  
Computational Study ◽  
Pyrimidine Dimer ◽  
Cyclobutane Pyrimidine Dimer

scholarly journals Action mechanism of Escherichia coli DNA photolyase. II. Role of the chromophores in catalysis.

1987 ◽  
Vol 262 (1) ◽  
pp. 486-491 ◽  
Author(s):  
M S Jorns ◽  
E T Baldwin ◽  
G B Sancar ◽  
A Sancar
Keyword(s):  
Escherichia Coli ◽  
Action Mechanism ◽  
Dna Photolyase
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