scholarly journals Observation of Intermolecular Interactions in Large Protein Complexes by 2D-Double Difference Nuclear Overhauser Enhancement Spectroscopy: Application to the 44 kDa Interferon–Receptor Complex

2011 ◽  
Vol 133 (37) ◽  
pp. 14755-14764 ◽  
Author(s):  
Ilona Nudelman ◽  
Sabine R. Akabayov ◽  
Tali Scherf ◽  
Jacob Anglister
Keyword(s):  
Intermolecular Interactions ◽  
Protein Complexes ◽  
Double Difference ◽  
Receptor Complex ◽  
Large Protein ◽  
Nuclear Overhauser Enhancement ◽  
Interferon Receptor ◽  
Nuclear Overhauser

scholarly journals Intermolecular Interactions in a 44 kDa Interferon−Receptor Complex Detected by Asymmetric Reverse-Protonation and Two-Dimensional NOESY

Biochemistry ◽  
2010 ◽  
Vol 49 (25) ◽  
pp. 5117-5133 ◽  
Author(s):  
Ilona Nudelman ◽  
Sabine R. Akabayov ◽  
Einat Schnur ◽  
Zohar Biron ◽  
Rina Levy ◽  
...  
Keyword(s):  
Intermolecular Interactions ◽  
Receptor Complex ◽  
Two Dimensional ◽  
Interferon Receptor ◽  
Reverse Protonation

scholarly journals Tafazzins from Drosophila and mammalian cells assemble in large protein complexes with a short half-life

Mitochondrion ◽  
2015 ◽  
Vol 21 ◽  
pp. 27-32 ◽  
Author(s):  
Yang Xu ◽  
Ashim Malhotra ◽  
Steven M. Claypool ◽  
Mindong Ren ◽  
Michael Schlame
Keyword(s):  
Half Life ◽  
Mammalian Cells ◽  
Protein Complexes ◽  
Large Protein ◽  
Short Half Life

scholarly journals The right place at the right time: Aurora B kinase localization to centromeres and kinetochores

2020 ◽  
Vol 64 (2) ◽  
pp. 299-311 ◽  
Author(s):  
Amanda J. Broad ◽  
Jennifer G. DeLuca
Keyword(s):  
Spindle Assembly Checkpoint ◽  
Protein Complexes ◽  
Centromeric Heterochromatin ◽  
Aurora B ◽  
Mitotic Chromosomes ◽  
Large Protein ◽  
Aurora B Kinase ◽  
Centromeric Protein ◽  
Spindle Microtubules ◽  
The Right

Abstract The fidelity of chromosome segregation during mitosis is intimately linked to the function of kinetochores, which are large protein complexes assembled at sites of centromeric heterochromatin on mitotic chromosomes. These key “orchestrators” of mitosis physically connect chromosomes to spindle microtubules and transduce forces through these connections to congress chromosomes and silence the spindle assembly checkpoint. Kinetochore-microtubule attachments are highly regulated to ensure that incorrect attachments are not prematurely stabilized, but instead released and corrected. The kinase activity of the centromeric protein Aurora B is required for kinetochore-microtubule destabilization during mitosis, but how the kinase acts on outer kinetochore substrates to selectively destabilize immature and erroneous attachments remains debated. Here, we review recent literature that sheds light on how Aurora B kinase is recruited to both centromeres and kinetochores and discuss possible mechanisms for how kinase interactions with substrates at distinct regions of mitotic chromosomes are regulated.

Sign in / Sign up

Export Citation Format

Share Document