In Situ Measurements of the Formation and Morphology of Intracellular β-Amyloid Fibrils by Super-Resolution Fluorescence Imaging

2011 ◽  
Vol 133 (33) ◽  
pp. 12902-12905 ◽  
Author(s):  
Gabriele S. Kaminski Schierle ◽  
Sebastian van de Linde ◽  
Miklos Erdelyi ◽  
Elin K. Esbjörner ◽  
Teresa Klein ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Amyloid Fibrils ◽  
Super Resolution ◽  
In Situ Measurements ◽  
Β Amyloid

scholarly journals Reconstruction of super-resolution ocean <i>p</i>CO<sub>2</sub> and air–sea fluxes of CO<sub>2</sub> from satellite imagery in the southeastern Atlantic

2015 ◽  
Vol 12 (17) ◽  
pp. 5229-5245 ◽  
Author(s):  
I. Hernández-Carrasco ◽  
J. Sudre ◽  
V. Garçon ◽  
H. Yahia ◽  
C. Garbe ◽  
...  
Keyword(s):  
Surface Temperature ◽  
Partial Pressure ◽  
Sea Surface Temperature ◽  
Ocean Color ◽  
Super Resolution ◽  
In Situ Measurements ◽  
Sea Surface ◽  
Co2 Fluxes ◽  
Partial Pressure Of Co2

Abstract. An accurate quantification of the role of the ocean as source/sink of greenhouse gases (GHGs) requires to access the high-resolution of the GHG air–sea flux at the interface. In this paper we present a novel method to reconstruct maps of surface ocean partial pressure of CO2 ( pCO2) and air–sea CO2 fluxes at super resolution (4 km, i.e., 1/32° at these latitudes) using sea surface temperature (SST) and ocean color (OC) data at this resolution, and CarbonTracker CO2 fluxes data at low resolution (110 km). Inference of super-resolution pCO2 and air–sea CO2 fluxes is performed using novel nonlinear signal processing methodologies that prove efficient in the context of oceanography. The theoretical background comes from the microcanonical multifractal formalism which unlocks the geometrical determination of cascading properties of physical intensive variables. As a consequence, a multi-resolution analysis performed on the signal of the so-called singularity exponents allows for the correct and near optimal cross-scale inference of GHG fluxes, as the inference suits the geometric realization of the cascade. We apply such a methodology to the study offshore of the Benguela area. The inferred representation of oceanic partial pressure of CO2 improves and enhances the description provided by CarbonTracker, capturing the small-scale variability. We examine different combinations of ocean color and sea surface temperature products in order to increase the number of valid points and the quality of the inferred pCO2 field. The methodology is validated using in situ measurements by means of statistical errors. We find that mean absolute and relative errors in the inferred values of pCO2 with respect to in situ measurements are smaller than for CarbonTracker.

Single-wavelength-controlled in situ dynamic super-resolution fluorescence imaging for block copolymer nanostructures via blue-light-switchable FRAP

2016 ◽  
Vol 15 (11) ◽  
pp. 1433-1441 ◽  
Author(s):  
Wen-Liang Gong ◽  
Jie Yan ◽  
Ling-Xi Zhao ◽  
Chong Li ◽  
Zhen-Li Huang ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Block Copolymers ◽  
Fluorescence Imaging ◽  
Blue Light ◽  
Super Resolution ◽  
Resolution Imaging

A blue-light-switchable fluorophore enables single-wavelength controlledin situdynamic super-resolution imaging of block copolymers.

scholarly journals Reconstruction of super-resolution fields of ocean <i>p</i>CO<sub>2</sub> and air–sea fluxes of CO<sub>2</sub> from satellite imagery in the Southeastern Atlantic

2015 ◽  
Vol 12 (2) ◽  
pp. 1405-1452 ◽  
Author(s):  
I. Hernández-Carrasco ◽  
J. Sudre ◽  
V. Garçon ◽  
H. Yahia ◽  
C. Garbe ◽  
...  
Keyword(s):  
Surface Temperature ◽  
Partial Pressure ◽  
Sea Surface Temperature ◽  
Super Resolution ◽  
In Situ Measurements ◽  
Sea Surface ◽  
Co2 Fluxes ◽  
Ocean Colour ◽  
Partial Pressure Of Co2

Abstract. The knowledge of Green House Gases GHGs fluxes at the air–sea interface at high resolution is crucial to accurately quantify the role of the ocean in the absorption and emission of GHGs. In this paper we present a novel method to reconstruct maps of surface ocean partial pressure of CO2, pCO2, and air–sea CO2 fluxes at super resolution (4 km) using Sea Surface Temperature (SST) and Ocean Colour (OC) data at this resolution, and CarbonTracker CO2 fluxes data at low resolution (110 km). Inference of super-resolution of pCO2, and air–sea CO2 fluxes is performed using novel nonlinear signal processing methodologies that prove efficient in the context of oceanography. The theoretical background comes from the Microcanonical Multifractal Formalism which unlocks the geometrical determination of cascading properties of physical intensive variables. As a consequence, a multiresolution analysis performed on the signal of the so-called singularity exponents allows the correct and near optimal cross-scale inference of GHGs fluxes, as the inference suits the geometric realization of the cascade. We apply such a methodology to the study offshore of the Benguela area. The inferred representation of oceanic partial pressure of CO2 improves and enhances the description provided by CarbonTracker, capturing the small scale variability. We examine different combinations of Ocean Colour and Sea Surface Temperature products in order to increase the number of valid points and the quality of the inferred pCO2 field. The methodology is validated using in-situ measurements by means of statistical errors. We obtain that mean absolute and relative errors in the inferred values of pCO2 with respect to in-situ measurements are smaller than for CarbonTracker.

The Carbon:²³⁴Thorium ratios of sinking particles in the California Current Ecosystem 2: Examination of a thorium sorption, desorption, and particle transport model

2019 ◽  
Author(s):  
Michael Stukel ◽  
Thomas Kelly
Keyword(s):  
Organic Carbon ◽  
Water Column ◽  
Particulate Organic Carbon ◽  
Transport Model ◽  
California Current ◽  
In Situ Measurements ◽  
Power Law Distribution ◽  
Sinking Particles ◽  
Organic Carbon Concentration

Thorium-234 (234Th) is a powerful tracer of particle dynamics and the biological pump in the surface ocean; however, variability in carbon:thorium ratios of sinking particles adds substantial uncertainty to estimates of organic carbon export. We coupled a mechanistic thorium sorption and desorption model to a one-dimensional particle sinking model that uses realistic particle settling velocity spectra. The model generates estimates of 238U-234Th disequilibrium, particulate organic carbon concentration, and the C:234Th ratio of sinking particles, which are then compared to in situ measurements from quasi-Lagrangian studies conducted on six cruises in the California Current Ecosystem. Broad patterns observed in in situ measurements, including decreasing C:234Th ratios with depth and a strong correlation between sinking C:234Th and the ratio of vertically-integrated particulate organic carbon (POC) to vertically-integrated total water column 234Th, were accurately recovered by models assuming either a power law distribution of sinking speeds or a double log normal distribution of sinking speeds. Simulations suggested that the observed decrease in C:234Th with depth may be driven by preferential remineralization of carbon by particle-attached microbes. However, an alternate model structure featuring complete consumption and/or disaggregation of particles by mesozooplankton (e.g. no preferential remineralization of carbon) was also able to simulate decreasing C:234Th with depth (although the decrease was weaker), driven by 234Th adsorption onto slowly sinking particles. Model results also suggest that during bloom decays C:234Th ratios of sinking particles should be higher than expected (based on contemporaneous water column POC), because high settling velocities minimize carbon remineralization during sinking.

Evaluation of reanalysis and satellite-based sea surface winds using in situ measurements from Chinese Antarctic Expeditions

2013 ◽  
Vol 24 (3) ◽  
pp. 147
Author(s):  
Ming LI ◽  
Qinghua YANG ◽  
Jiechen ZHAO ◽  
Lin ZHANG ◽  
Chunhua LI ◽  
...  
Keyword(s):  
In Situ Measurements ◽  
Sea Surface ◽  
Surface Winds ◽  
Sea Surface Winds

The effects of surcharged manholes on the travel time and dispersion of solutes in sewer systems

1995 ◽  
Vol 31 (7) ◽  
pp. 51-59 ◽  
Author(s):  
Ian Guymer ◽  
Rob O'Brien
Keyword(s):  
Travel Time ◽  
Moment Method ◽  
In Situ Measurements ◽  
Time Change ◽  
Longitudinal Dispersion ◽  
Laboratory System ◽  
Environmental Load ◽  
Hydraulic Characteristics ◽  
Sewer Systems

Previously, the design of sewer systems has been limited to studies of their hydraulic characteristics, in particular the ability of the system to convey the maximum discharge. Greater environmental awareness has necessitated that new designs, and some existing schemes, are assessed to determine the environmental load which the scheme will deliver to any downstream component. This paper describes a laboratory programme which has been designed to elucidate the effects of manholes on the longitudinal dispersion of solutes. A laboratory system is described, which allows in situ measurements to be taken of the concentration of a fluorescent solute tracer, both up- and down-stream of a surcharged manhole junction. Results are presented from a preliminary series of studies undertaken for a single manhole geometry over a range of discharges, with varying levels of surcharge. Results are presented showing the variation of travel time, change in second moment of the distribution and of a dispersion factor with surcharge, assuming a Taylor approach and determining the dispersion factor using a ‘change in moment’ method. The effect of the stored volume within the manhole is clearly evident. The limitations and the applicability of this approach are discussed.

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