scholarly journals Molecular Mechanochemistry: Low Force Switch Slows Enzymatic Cleavage of Human Type I Collagen Monomer

2011 ◽  
Vol 133 (11) ◽  
pp. 4073-4078 ◽  
Author(s):  
Robert J. Camp ◽  
Melody Liles ◽  
John Beale ◽  
Nima Saeidi ◽  
Brendan P. Flynn ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Type I ◽  
Enzymatic Cleavage ◽  
Human Type

scholarly journals Functional analysis of cis-acting DNA sequences controlling transcription of the human type I collagen genes.

1990 ◽  
Vol 265 (22) ◽  
pp. 13351-13356
Author(s):  
S Boast ◽  
M W Su ◽  
F Ramirez ◽  
M Sanchez ◽  
E V Avvedimento
Keyword(s):  
Functional Analysis ◽  
Dna Sequences ◽  
Type I Collagen ◽  
Type I ◽  
Cis Acting ◽  
Human Type ◽  
Collagen Genes

scholarly journals A Novel Role of Annexin A2 in Human Type I Collagen Gene Expression

2015 ◽  
Vol 116 (3) ◽  
pp. 408-417 ◽  
Author(s):  
Georgia Schäfer ◽  
Jessica K. Hitchcock ◽  
Tamlyn M. Shaw ◽  
Arieh A. Katz ◽  
M. Iqbal Parker
Keyword(s):  
Gene Expression ◽  
Type I Collagen ◽  
Annexin A2 ◽  
Type I ◽  
Collagen Gene ◽  
Human Type ◽  
Collagen Gene Expression

High-level production of human type I collagen in the yeastPichia pastoris

Yeast ◽  
2001 ◽  
Vol 18 (9) ◽  
pp. 797-806 ◽  
Author(s):  
Minna Nokelainen ◽  
Hongmin Tu ◽  
Annamari Vuorela ◽  
Holger Notbohm ◽  
Kari I. Kivirikko ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Type I ◽  
Human Type ◽  
High Level ◽  
Level Production

Association of Functional Microsatellites in the Human Type I Collagen α2 Chain (COL1A2) Gene with Systemic Sclerosis

2000 ◽  
Vol 272 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Ryu-Ichiro Hata ◽  
Jun Akai ◽  
Akinori Kimura ◽  
Osamu Ishikawa ◽  
Masataka Kuwana ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Type I Collagen ◽  
Type I ◽  
Human Type ◽  
Col1a2 Gene

Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen

1990 ◽  
Vol 36 (7) ◽  
pp. 1328-1332 ◽  
Author(s):  
J Melkko ◽  
S Niemi ◽  
L Risteli ◽  
J Risteli
Keyword(s):  
Type I Collagen ◽  
Soft Tissues ◽  
Polyacrylamide Gel Electrophoresis ◽  
Primary Cultures ◽  
Sodium Dodecyl ◽  
Type I ◽  
Lectin Affinity Chromatography ◽  
Human Type ◽  
Serum Antigen ◽  
Type I Procollagen

Abstract Type I collagen is the most abundant collagen type in soft tissues and the only type found in mineralized bone. We established a rapid equilibrium radioimmunoassay for the carboxyterminal propeptide of human type I procollagen (PICP), to be used as an indicator of the synthesis of type I collagen. We isolated type I procollagen from the medium of primary cultures of human skin fibroblasts, digested the protein with highly purified bacterial collagenase, and purified PICP by lectin-affinity chromatography, gel filtration, and ion-exchange separation on HPLC. The purity of the protein was verified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by N-terminal amino acid sequencing of its component chains. The final radioimmunoassay was established with polyclonal rabbit antibodies. Material antigenically related to PICP is readily detected in human serum. There is only one form of the serum antigen, its molecular size and affinity to the antibodies being similar to those of the isolated propeptide. Intra- and interassay CVs are 3% and 5%, respectively. Preliminary reference intervals for healthy adults (18 to 61 years of age) are 38-202 micrograms/L for men and 50-170 micrograms/L for women: in men the concentration is inversely related to age. The serum antigen is stable during storage and after repeated thawing.

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