19F NMR Analysis of the Antimicrobial Peptide PGLa Bound to Native Cell Membranes from Bacterial Protoplasts and Human Erythrocytes

2010 ◽  
Vol 132 (26) ◽  
pp. 8822-8824 ◽  
Author(s):  
Marco Ieronimo ◽  
Sergii Afonin ◽  
Katja Koch ◽  
Marina Berditsch ◽  
Parvesh Wadhwani ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Cell Membranes ◽  
Human Erythrocytes ◽  
19F Nmr ◽  
Nmr Analysis ◽  
Native Cell

scholarly journals Sterol structural requirements for inhibition of streptolysin O activity

1974 ◽  
Vol 140 (1) ◽  
pp. 95-98 ◽  
Author(s):  
Kenneth C. Watson ◽  
Eric J. C. Kerr
Keyword(s):  
Cell Membranes ◽  
Human Erythrocytes ◽  
Hydroxyl Group ◽  
Membrane Cholesterol ◽  
Specific Requirement ◽  
Structural Requirements ◽  
Hydrophilic Group ◽  
Streptolysin O

Reduced streptolysin O, a toxin produced by certain β-haemolytic streptococci, lyses human erythrocytes. The reaction is inhibited by cholesterol at concentrations of about 1.0μg/ml. Other sterols inhibit the lysin and there is a specific requirement for a 3β-hydroxyl group. Inhibition was obtained with 3β-hydroxychol-5-en-24-oic acid, containing a hydrophilic group at C-24. The mode of inhibition is likely to involve attachment to the fixation site of the lysin which attaches the molecule to cell membranes, probably to membrane cholesterol. A second streptolysin site, concerned in the final haemolytic event, may also be involved. Inhibitors of the latter site have not been characterized, other than antibody with specificity for the site.

scholarly journals Study of the Reactivity of Lignin Model Compounds to Fluorobenzylation Using 13C and 19F NMR: Application to Lignin Phenolic Hydroxyl Group Quantification by 19F NMR

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3211
Author(s):  
Esakkiammal Sudha Esakkimuthu ◽  
Nathalie Marlin ◽  
Marie-Christine Brochier-Salon ◽  
Gérard Mortha
Keyword(s):  
Reaction Medium ◽  
19F Nmr ◽  
Hydroxyl Group ◽  
Hydroxyl Groups ◽  
Nmr Analysis ◽  
Model Compounds ◽  
Lignin Model Compounds ◽  
Wide Range ◽  
Lignin Model ◽  
13C And 19F Nmr

Lignin is an aromatic biopolymer derived from lignocellulosic biomass. Providing a comprehensive structural analysis of lignin is the primary motivation for the quantification of various functional groups, with a view to valorizing lignin in a wide range of applications. This study investigated the lignin fluorobenzylation reaction and performed a subsequent 19F-NMR analysis to quantify hydroxyl groups, based on a work developed two decades ago by Barrelle et al. The objectives were to check the assignments proposed in this previous study and to examine the reactivity of various types of lignin hydroxyls with the derivatization agent. Selected lignin model compounds containing phenolic and aliphatic hydroxyls were subjected to the fluorobenzylation reaction, and the obtained reaction medium was analyzed by 13C and 19F NMR spectroscopy. The model compound results showed that phenolic hydroxyls were totally derivatized, whereas aliphatic hydroxyls underwent minimal conversion. They also confirmed that 19F NMR chemical shifts from −115 ppm to −117.3 ppm corresponded to phenolic groups. Then, a 19F NMR analysis was successfully applied to Organosolv commercial lignin after fluorobenzylation in order to quantify its phenolic group content; the values were found to be in the range of the reported values using other analytical techniques after lignin acetylation.

scholarly journals 3P075 NMR analysis of the oligomerization mechanism of antimicrobial peptide human defensin 5(01D. Protein: Function,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

2014 ◽  
Vol 54 (supplement1-2) ◽  
pp. S261
Author(s):  
Arata Hashimoto ◽  
Satoshi Tomisawa ◽  
Masakatsu Kamiya ◽  
Takashi Kikukawa ◽  
Yasuhiro Kumaki ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Annual Meeting ◽  
Protein Function ◽  
Nmr Analysis ◽  
Biophysical Society ◽  
Human Defensin 5

Orientation of the antimicrobial peptide PGLa in lipid membranes determined from 19F-NMR dipolar couplings of 4-CF3-phenylglycine labels

2004 ◽  
Vol 168 (1) ◽  
pp. 153-163 ◽  
Author(s):  
Ralf W. Glaser ◽  
Carsten Sachse ◽  
Ulrich H.N. Dürr ◽  
Parvesh Wadhwani ◽  
Anne S. Ulrich
Keyword(s):  
Antimicrobial Peptide ◽  
Lipid Membranes ◽  
Dipolar Couplings ◽  
19F Nmr
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