An Adsorption-Based Model for Pulse Duration in Resistive-Pulse Protein Sensing

2010 ◽  
Vol 132 (19) ◽  
pp. 6755-6763 ◽  
Author(s):  
Lindsay T. Sexton ◽  
Hitomi Mukaibo ◽  
Parag Katira ◽  
Henry Hess ◽  
Stefanie A. Sherrill ◽  
...  
Keyword(s):  
Pulse Duration ◽  
Protein Sensing ◽  
Resistive Pulse ◽  
Pulse Protein

Laser pulse duration effects in Xe2+ ions induced by multiphoton absorption at 0.53 μm

1983 ◽  
Vol 44 (11) ◽  
pp. 1247-1255 ◽  
Author(s):  
A. L'Huillier ◽  
L.A. Lompre ◽  
G. Mainfray ◽  
C. Manus
Keyword(s):  
Laser Pulse ◽  
Pulse Duration ◽  
Laser Pulse Duration ◽  
Multiphoton Absorption

Rapid Quantification of Prion Proteins

2019 ◽  
Author(s):  
Matthew Healey ◽  
Muttuswamy Sivakumaran ◽  
Mark Platt
Keyword(s):  
Prion Proteins ◽  
Prion Diseases ◽  
Sample Volume ◽  
Cellular Prion Protein ◽  
Dna Aptamer ◽  
Superparamagnetic Beads ◽  
Resistive Pulse ◽  
Large Sample Volume ◽  
Complex Sample ◽  
Neurological Conditions

<p>Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of the normal intrinsic cellular prion protein (PrP<sup>C</sup>) in to the highly ordered insoluble amyloid state conformer (PrP<sup>SC</sup>). We present a rapid assay using Aptamers and Resistive Pulse Sensing, RPS, to extract and quantify proteins from complex sample matrices, demonstrate with the quantification of PrP<sup>c</sup>. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB’s termed P-Beads, are used to pre-concentrate the analyte from a large sample volume. The PrP<sup>c</sup> protein is then eluted from the P-Beads before aptamer modified sensing beads, S-Beads, are added. The velocity of the S-Beads through the nanopore reveals the concentration of the PrP<sup>c</sup> protein. The process is done in under an hour and allows the detection of picomol’s of protein. The technique could be easily adopted to the mutated version of the protein and integrated into clinical workflows for the screening of blood donations and transfusions. </p>

Conversion of pulse protein-based oleogels into oleofoams for improved bakery application

2020 ◽  
Author(s):  
Athira Mohanan ◽  
Michael T Nickerson ◽  
Supratim Ghosh
Keyword(s):  
Pulse Protein

P6595The optogenetic defibrillation of ventricular arrhythmia in myocardial infarction rats in vivo

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
X I Wang ◽  
Y Cheng ◽  
P Rao ◽  
L Wang
Keyword(s):  
Light Intensity ◽  
Ventricular Arrhythmia ◽  
Sinus Rhythm ◽  
Pulse Duration ◽  
Underlying Mechanism ◽  
Blue Laser ◽  
Continuous Illumination ◽  
Threshold Intensity ◽  
Systemic Delivery ◽  
Significant Difference

Abstract Introduction Optogenetics is a low-invasive, flexible and highly selective intervention that enables electrical excitation with light on myocardium overexpressing light-sensitive proteins. Optical illumination can control the simultaneous exciting of the whole myocardium under the spot, which is more conducive to recovery from electrical disturbance to sinus rhythm. Purpose We explored optogenetic defibrillation for different illumination parameters how to affect defibrillation rates and the possible mechanism of continuous illumination defibrillation. Methods Systemic delivery via right jugular vein injection of (AAV9-CAG-hChR2(H134R)-mCherry) were performed in juvenile SD rats to achieve the light sensitive protein Channelrhodopsin-2 (ChR2) transfer throughout the whole heart. We intubated and ventilated rats, opened chest and recorded the ECG. After ligation of the left anterior descending coronary artery, ventricular arrhythmia was induced by electrical burst stimulation (10v, 50Hz, 2s). Cardiac epicardium illumination with 470nm blue laser was performed to investigate the effects of optogenetic defibrillation and its underlying mechanism. Every heart accepted 30 pulses of 20ms duration on 8Hz to test the light intensity threshold for 1:1 capture. Different illumination modes of multiple light intensity (2,4,8,10,20 times threshold intensity), pulse duration (20, 50, 200, 500 and 1000ms) and illumination position (RV apex, RV, RVOT, septum, LV) were applied in each attempt for 4 repetitions with 1 s interval. Results We demonstrated that ventricular arrhythmias could be terminated by illumination of the right ventricle at 20 times threshold intensity in 1s (figure A) with the successful defibrillation rate of 95±2.673% (mean ±SEM; N=7). Herein, the successful optogenetic defibrillation rate was strongly depending on light intensity (N=5, n=50 episodes, p=0.0118) and duration of illumination (N=5, n=50 episodes, p<0.0001) (figure B.C). Notably when there were higher intensity and longer pulse duration, the higher defibrillation rate appeared. There was no significant difference in the defibrillation rate among different illumination positions (N=5, n=25episodes per position, p=0.1177) (figure D). To explore the underlying mechanism of optogenetic defibrillation, we performed the same illumination mode during sinus rhythm in 2 rats (figure E. F. G). We observed that higher light intensity and longer pulse duration were more conducive to induce an episode of higher frequency focal excitement. Views of optogenetic defibrillation Conclusions We demonstrated that optogenetic defibrillation is a highly effective intervention and the possible mechanism is partly attributed to overdrive suppression. We believe that optogenetic approach is potentially to be translated into more efficient and pain-free clinical termination of ventricular arrhythmia. Acknowledgement/Funding The national natural science foundation of China (81772044)

scholarly journals OBP14 (Odorant-Binding Protein) Sensing in Adelphocoris lineolatus Based on Peptide Nucleic Acid and Graphene Oxide

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 422
Author(s):  
Wenhua Tian ◽  
Tao Zhang ◽  
Shaohua Gu ◽  
Yuyuan Guo ◽  
Xiwu Gao ◽  
...  
Keyword(s):  
Peptide Nucleic Acid ◽  
Antennal Lobe ◽  
Behavioral Regulation ◽  
Dual Roles ◽  
Protein Sensing ◽  
Physiological Functions ◽  
Adelphocoris Lineolatus ◽  
Regulation Strategies ◽  
Odorant Binding

OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in A. lineolatus, the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on AlinOBP14, a Plus-C OBP from A. lineolatus, and we developed a PNA-GO-based mRNA biosensor to detect the expression of AlinOBP14. The results demonstrated that AlinOBP14 plays dual roles in A. lineolatus. The AlinOBP14 is expressed beneath the epidermis of the vertex and gena in heads of A. lineolatus, and it functions as a carrier for three terpenoids, while AlinOBP14 is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHⅢ. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest.

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