scholarly journals Recognition of the Ring-Opened State of Proliferating Cell Nuclear Antigen by Replication Factor C Promotes Eukaryotic Clamp-Loading

2010 ◽  
Vol 132 (21) ◽  
pp. 7372-7378 ◽  
Author(s):  
John A. Tainer ◽  
J. Andrew McCammon ◽  
Ivaylo Ivanov
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Replication Factor C ◽  
Replication Factor ◽  
Clamp Loading ◽  
Factor C ◽  
Proliferating Cell

scholarly journals Synthetic Activity ofSsoDNA Polymerase Y1, an Archaeal DinB-like DNA Polymerase, Is Stimulated by Processivity Factors Proliferating Cell Nuclear Antigen and Replication Factor C

2001 ◽  
Vol 276 (50) ◽  
pp. 47394-47401 ◽  
Author(s):  
Petr Grúz ◽  
Francesca M. Pisani ◽  
Masatomi Shimizu ◽  
Masami Yamada ◽  
Ikuko Hayashi ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Dna Polymerases ◽  
Nuclear Antigen ◽  
Synthetic Activity ◽  
Replication Factor C ◽  
Replication Factor ◽  
Pol Iv ◽  
Factor C ◽  
Proliferating Cell

DNA replication efficiency is dictated by DNA polymerases (pol) and their associated proteins. The recent discovery of DNA polymerase Y family (DinB/UmuC/RAD30/REV1 superfamily) raises a question of whether the DNA polymerase activities are modified by accessory proteins such as proliferating cell nuclear antigen (PCNA). In fact, the activity of DNA pol IV (DinB) ofEscherichia coliis enhanced upon interaction with the β subunit, the processivity factor of DNA pol III. Here, we report the activity ofSsoDNA pol Y1 encoded by thedbhgene of the archaeonSulfolobus solfataricusis greatly enhanced by the presence of PCNA and replication factor C (RFC).Ssopol Y1per sewas a distributive enzyme but a substantial increase in the processivity was observed on poly(dA)-oligo(dT) in the presence of PCNA (039p or 048p) and RFC. The length of the synthesized DNA product reached at least 200 nucleotides.Ssopol Y1 displayed a higher affinity for DNA compared with pol IV ofE. coli, suggesting that the two DNA polymerases have distinct reason(s) to require the processivity factors for efficient DNA synthesis. The abilities of pol Y1 and pol IV to bypass DNA lesions and their sensitive sites to protease are also discussed.

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