Ligand Specificity of CS-35, a Monoclonal Antibody That Recognizes Mycobacterial Lipoarabinomannan:  A Model System for Oligofuranoside−Protein Recognition

2007 ◽  
Vol 129 (34) ◽  
pp. 10489-10502 ◽  
Author(s):  
Christoph Rademacher ◽  
Glen K. Shoemaker ◽  
Hyo-Sun Kim ◽  
Ruixiang Blake Zheng ◽  
Hashem Taha ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Model System ◽  
Protein Recognition ◽  
Ligand Specificity

scholarly journals Direct immunocytochemistry with a horseradish peroxidase-conjugated monoclonal antibody against substance P.

1982 ◽  
Vol 30 (12) ◽  
pp. 1211-1216 ◽  
Author(s):  
D M Boorsma ◽  
A C Cuello ◽  
F W van Leeuwen
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Substance P ◽  
Horseradish Peroxidase ◽  
Nerve Fibers ◽  
Model System ◽  
Gel Chromatography ◽  
Rat Spinal Cord ◽  
Fixed Tissue ◽  
Triton X

The procedure for the isolation and conjugation of the anti-substance P monoclonal antibody NC1/34 with the enzyme horseradish peroxidase (HRP) is described. This resulted in a molecular complex of monoclonal antibody/HRP of 1:1. This conjugate was of approximately 400,000 daltons, as estimated by gel chromatography. Practically all the isolated antibody was coupled to HRP. The conjugate was tested both in a model system where CNBr-activated Sepharose beads were coupled to substance P and on fixed tissue preparations from the rat spinal cord and medulla oblongata. The conjugate revealed staining in nerve fibers in areas known to contain substance P. The best immunohistochemical results were obtained by prolonged incubations at 12 degrees C in the presence of 0.1% Triton X-100. The preabsorption of the conjugate with substance P obliterated the reaction.

scholarly journals Elimination of Daudi lymphoblasts from human bone marrow using avidin- biotin immunoadsorption

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 509-515
Author(s):  
RJ Berenson ◽  
WI Bensinger ◽  
D Kalamasz ◽  
P Martin
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Mononuclear Cells ◽  
Model System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Hematopoietic Progenitors ◽  
Fluorescent Microscope ◽  
Novel Approach ◽  
Daudi Cells

Biotinylated antibodies and an avidin-Sepharose 6MB column were utilized in a novel approach to deplete selected cell populations from human bone marrow. Fluorescein-labeled Daudi lymphoblasts were mixed with bone marrow mononuclear cells in a model system, and removal of Daudi cells was quantitatively assessed with an inverted fluorescent microscope. Treatment using the biotinylated monoclonal antibody 2H7 reactive with Bp32 antigen (expressed on Daudi cells) followed by passage over avidin-Sepharose produced greater than two logs of Daudi cell removal from bone marrow. An alternative method was tested by treating cells successively with nonbiotinylated monoclonal antibody and biotinylated goat antimouse immunoglobulin followed by passage over avidin-Sepharose. Up to three logs of Daudi cells could be eliminated from bone marrow with quantitative recovery of hematopoietic progenitors. The use of biotinylated goat antimouse immunoglobulin eliminates the need to prepare a biotin conjugate of each individual monoclonal antibody. The clinical application of cellular immunoadsorption using the avidin-biotin system may prove useful in bone marrow transplantation.

scholarly journals Molecular Basis for Species and Ligand Specificity of a Monoclonal Antibody Raised Against Human IGF-I

Endocrinology ◽  
1997 ◽  
Vol 138 (10) ◽  
pp. 4521-4523 ◽  
Author(s):  
Judson J. Van Wyk ◽  
Eyvonne T. Bruton
Keyword(s):  
Monoclonal Antibody ◽  
Aspartic Acid ◽  
Molecular Basis ◽  
Ligand Specificity ◽  
Critical Importance ◽  
High Concentration ◽  
Igf I ◽  
Very High

Abstract The anti-hIGF-I monoclonal antibody, α-sm1.2, was found to have substantial crossreactivity with human and rat IGF-II, but recognized rat IGF-I only when this ligand was present at very high concentration. (E50 for hIGF-I ∼3.5 ng/tube vs. ∼12,000 ng/tube for rat IGF-I). In the context of previous studies to define the epitope(s) of α-sm1.2, these findings point to the critical importance of aspartic acid at residue 20 in the B domain in determining the species and ligand specificity of this antibody. Previous studies using this antibody in rodent tissues may require reinterpretation in the light of these findings.

Clogging of microfluidic constrictions by monoclonal antibody aggregates: role of aggregate shape and deformability

Soft Matter ◽  
2020 ◽  
Vol 16 (4) ◽  
pp. 921-928 ◽  
Author(s):  
Charles Duchêne ◽  
Vasco Filipe ◽  
Sylvain Huille ◽  
Anke Lindner
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Applied Pressure ◽  
Model System ◽  
Aggregate Shape

Using a microfluidic model system, we demonstrate that aggregates formed in solutions of monoclonal antibodies geometrically clog constrictions and that unclogging can be obtained by increasing the applied pressure through aggregate deformability.

scholarly journals Elimination of Daudi lymphoblasts from human bone marrow using avidin- biotin immunoadsorption

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 509-515 ◽  
Author(s):  
RJ Berenson ◽  
WI Bensinger ◽  
D Kalamasz ◽  
P Martin
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Mononuclear Cells ◽  
Model System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Hematopoietic Progenitors ◽  
Fluorescent Microscope ◽  
Novel Approach ◽  
Daudi Cells

Abstract Biotinylated antibodies and an avidin-Sepharose 6MB column were utilized in a novel approach to deplete selected cell populations from human bone marrow. Fluorescein-labeled Daudi lymphoblasts were mixed with bone marrow mononuclear cells in a model system, and removal of Daudi cells was quantitatively assessed with an inverted fluorescent microscope. Treatment using the biotinylated monoclonal antibody 2H7 reactive with Bp32 antigen (expressed on Daudi cells) followed by passage over avidin-Sepharose produced greater than two logs of Daudi cell removal from bone marrow. An alternative method was tested by treating cells successively with nonbiotinylated monoclonal antibody and biotinylated goat antimouse immunoglobulin followed by passage over avidin-Sepharose. Up to three logs of Daudi cells could be eliminated from bone marrow with quantitative recovery of hematopoietic progenitors. The use of biotinylated goat antimouse immunoglobulin eliminates the need to prepare a biotin conjugate of each individual monoclonal antibody. The clinical application of cellular immunoadsorption using the avidin-biotin system may prove useful in bone marrow transplantation.

A Protozoan Model for Golgi-Associated Calcification

1971 ◽  
Vol 29 ◽  
pp. 332-333
Author(s):  
D. C. Williams ◽  
D. E. Outka
Keyword(s):  
Golgi Apparatus ◽  
Organic Matrix ◽  
Model System ◽  
Sequential Formation

Many studies have shown that the Golgi apparatus is involved in a variety of synthetic activities, and probably no Golgi product is more elaborate than the scales produced by various kinds of phytoflagellates. The formation of calcified scales (coccoliths, Fig. 1,2) of the coccolithophorid phytoflagellates provides a particularly interesting model system for the study of biological mineralization, and the sequential formation of Golgi products.The coccoliths of Hymenomonas carterae consist of a scale-like base (Fig. 2 and 4, b) with a highly structured calcified (CaCO3) rim composed of two distinct elements which alternate about the base periphery (Fig. 1 and 3, A, B). Each element is enveloped by a sheath-like organic matrix (Fig. 3; Fig. 4, m).

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