scholarly journals Regiospecific Chlorination of (S)-β-Tyrosyl-S-Carrier Protein Catalyzed by SgcC3 in the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027

2007 ◽  
Vol 129 (41) ◽  
pp. 12432-12438 ◽  
Author(s):  
Shuangjun Lin ◽  
Steven G. Van Lanen ◽  
Ben Shen
Keyword(s):  
Carrier Protein ◽  
Antitumor Antibiotic

Biosynthesis of the β-Amino Acid Moiety of the Enediyne Antitumor Antibiotic C-1027 Featuring β-Amino Acyl-S-carrier Protein Intermediates

2005 ◽  
Vol 127 (33) ◽  
pp. 11594-11595 ◽  
Author(s):  
Steven G. Van Lanen ◽  
Pieter C. Dorrestein ◽  
Steve D. Christenson ◽  
Wen Liu ◽  
Jianhua Ju ◽  
...  
Keyword(s):  
Amino Acid ◽  
Carrier Protein ◽  
Antitumor Antibiotic ◽  
Acid Moiety ◽  
Amino Acid Moiety

Importance of Protease Inhibition in Studies on Purified Factor VIII (Antihaemophilic Factor)

1976 ◽  
Vol 35 (01) ◽  
pp. 186-190 ◽  
Author(s):  
Eugen A. Beck ◽  
Peter Bachmann ◽  
Peter Barbier ◽  
Miha Furlan
Keyword(s):  
Ionic Strength ◽  
Factor Viii ◽  
Gel Filtration ◽  
High Ionic Strength ◽  
Procoagulant Activity ◽  
Carrier Protein ◽  
Protease Inhibition ◽  
Functional Sites ◽  
Molecular Weight Peak ◽  
Von Willebrand

SummaryAccording to some authors factor VIII procoagulant activity may be dissociable from carrier protein (MW~ 2 × 106) by agarose gel filtration, e.g. at high ionic strength. We were able to reproduce this phenomenon. However, addition of protease inhibitor (Trasylol) prevented the appearance of low molecular weight peak of factor VIII procoagulant activity both at high ionic strength and elevated temperature (37°C). We conclude from our results that procoagulant activity and carrier protein (von Willebrand factor, factor VIII antigen) are closely associated functional sites of native factor VIII macro molecule. Consequently, proteolytic degradation should be avoided in functional and structural studies on factor VIII and especially in preparing factor VIII concentrate for therapeutic use.

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