scholarly journals Phage Display Evolution of a Peptide Substrate for Yeast Biotin Ligase and Application to Two-Color Quantum Dot Labeling of Cell Surface Proteins

2007 ◽  
Vol 129 (20) ◽  
pp. 6619-6625 ◽  
Author(s):  
Irwin Chen ◽  
Yoon-Aa Choi ◽  
Alice Y. Ting
Keyword(s):  
Quantum Dot ◽  
Cell Surface ◽  
Phage Display ◽  
Surface Proteins ◽  
Peptide Substrate ◽  
Cell Surface Proteins ◽  
Biotin Ligase

Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase

2005 ◽  
Vol 2 (2) ◽  
pp. 99-104 ◽  
Author(s):  
Irwin Chen ◽  
Mark Howarth ◽  
Weiying Lin ◽  
Alice Y Ting
Keyword(s):  
Cell Surface ◽  
Surface Proteins ◽  
Cell Surface Proteins ◽  
Site Specific ◽  
Biotin Ligase ◽  
Biophysical Probes

scholarly journals Quantum Dot Probes for Single-Molecule Rotation of Cell Surface Proteins

2010 ◽  
Vol 98 (3) ◽  
pp. 591a-592a
Author(s):  
Peter W. Winter ◽  
Deborah A. Roess ◽  
B. George Barisas
Keyword(s):  
Quantum Dot ◽  
Cell Surface ◽  
Single Molecule ◽  
Surface Proteins ◽  
Cell Surface Proteins

Shot-gun phage display mapping of two streptococcal cell-surface proteins

1997 ◽  
Vol 152 (2) ◽  
pp. 121-128 ◽  
Author(s):  
Karin Jacobsson ◽  
Hans Jonsson ◽  
Hans Lindmark ◽  
Bengt Guss ◽  
Martin Lindberg ◽  
...  
Keyword(s):  
Cell Surface ◽  
Phage Display ◽  
Surface Proteins ◽  
Cell Surface Proteins

Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates

2005 ◽  
pp. 035-050 ◽  
Author(s):  
John N. Mason ◽  
Ian D. Tomlinson ◽  
Sandra J. Rosenthal ◽  
Randy D. Blakely
Keyword(s):  
Quantum Dot ◽  
Cell Surface ◽  
Surface Proteins ◽  
Cell Surface Proteins

What do nanometer molecular trajectories tell us about heterogeneous cell surface domaines?

1995 ◽  
Vol 53 ◽  
pp. 786-787
Author(s):  
Watt W. Webb
Keyword(s):  
Cell Surface ◽  
Lipid Membranes ◽  
Surface Proteins ◽  
Protein Diffusion ◽  
Coated Pits ◽  
Cell Surface Proteins ◽  
Membrane Heterogeneity ◽  
Fluorescence Photobleaching Recovery ◽  
Photobleaching Recovery ◽  
Plasma Membrane Heterogeneity

Plasma membrane heterogeneity is implicit in the existence of specialized cell surface organelles which are necessary for cellular function; coated pits, post and pre-synaptic terminals, microvillae, caveolae, tight junctions, focal contacts and endothelial polarization are examples. The persistence of these discrete molecular aggregates depends on localized restraint of the constituent molecules within specific domaines in the cell surface by strong intermolecular bonds and/or anchorage to extended cytoskeleton. The observed plasticity of many of organelles and the dynamical modulation of domaines induced by cellular signaling evidence evanescent intermolecular interactions even in conspicuous aggregates. There is also strong evidence that universal restraints on the mobility of cell surface proteins persist virtually everywhere in cell surfaces, not only in the discrete organelles. Diffusion of cell surface proteins is slowed by several orders of magnitude relative to corresponding protein diffusion coefficients in isolated lipid membranes as has been determined by various ensemble average methods of measurement such as fluorescence photobleaching recovery(FPR).

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