Noninvasive Probing of the Spatial Organization of Polymer Chains in Hydrogels Using Fluorescence Resonance Energy Transfer (FRET)

2007 ◽  
Vol 129 (15) ◽  
pp. 4518-4519 ◽  
Author(s):  
Hyun Joon Kong ◽  
Chan Joong Kim ◽  
Nathaniel Huebsch ◽  
David Weitz ◽  
David J. Mooney
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Spatial Organization ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Polymer Chains ◽  
Fluorescence Resonance

scholarly journals Conformational Transitions of Polymer Chains in Solutions Characterized by Fluorescence Resonance Energy Transfer

Polymers ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1007 ◽  
Author(s):  
Linlin Qin ◽  
Linling Li ◽  
Ye Sha ◽  
Ziyu Wang ◽  
Dongshan Zhou ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Conformational Changes ◽  
Conformational Transition ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Polymer Chains ◽  
Donor And Acceptor ◽  
Transition Concentration ◽  
Fluorescence Resonance

The critical overlap concentration C* is an important concept in polymer solutions and is defined as the boundary between dilute and semidilute regimes. In this study, the chain conformational changes of polystyrene (PS) with both high (Mn = 200,000 Da) and low (Mn = 13,000 Da) molecular weights in cis-decalin were compared by intrachain fluorescence resonance energy transfer (FRET). The random labeling of donor and acceptor chromophores strategy was employed for long PS chains, whereas chain-end labeling was used for short PS chains. By monitoring the spectroscopic intensity ratio between acceptor and donor, the concentration dependence on chain conformation from dilute to semidilute solutions was determined. Both long and short chains exhibit a conformational transition concentration, above which the polymer chains begin to collapse with concentration significantly. Interestingly, for randomly labeled polymer long chains, such concentration is consistent with C* determined from the viscosity result, below which only slight conformational change of polymer chain takes place. However, for the chain-end labeled short chain, the conformational transition concentration takes place earlier than C*, below which no significant polymer conformation change is observed.

scholarly journals A Fluorescence Resonance Energy Transfer-Based Analytical Tool for Nitrate Quantification in Living Cells

ACS Omega ◽  
2020 ◽  
Vol 5 (46) ◽  
pp. 30306-30314
Author(s):  
Urooj Fatima ◽  
Fuad Ameen ◽  
Neha Soleja ◽  
Parvez Khan ◽  
Abobakr Almansob ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Analytical Tool ◽  
Fluorescence Resonance

scholarly journals Graphdiyne nanosheets as a platform for accurate copper(ii) ion detection via click chemistry and fluorescence resonance energy transfer

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5320-5324
Author(s):  
Chenchen Ge ◽  
Jiaofu Li ◽  
Dou Wang ◽  
Kongpeng Lv ◽  
Quan Liu ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Quenching ◽  
Click Chemistry ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Specificity ◽  
Ion Detection ◽  
Fluorescence Resonance

Cu2+ detection was performed by taking advantage of the fluorescence quenching ability of graphdiyne and the high specificity of click chemistry.

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