Site-Specific Incorporation of a19F-Amino Acid into Proteins as an NMR Probe for Characterizing Protein Structure and Reactivity

2007 ◽  
Vol 129 (5) ◽  
pp. 1160-1166 ◽  
Author(s):  
Jennifer C. Jackson ◽  
Jared T. Hammill ◽  
Ryan A. Mehl
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Site Specific

Site Specific Incorporation of Amino Acid Analogues into Proteins In Vivo

2000 ◽  
Author(s):  
Anne K. Kowal ◽  
Caroline Kohrer ◽  
Uttam L. RajBhandary
Keyword(s):  
Amino Acid ◽  
Site Specific ◽  
Amino Acid Analogues

scholarly journals Codon harmonization reduces amino acid misincorporation in bacterially expressed P. falciparum proteins and improves their immunogenicity

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Neeraja Punde ◽  
Jennifer Kooken ◽  
Dagmar Leary ◽  
Patricia M. Legler ◽  
Evelina Angov
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Codon Usage ◽  
Dna Sequences ◽  
Structural Integrity ◽  
Host Cells ◽  
Loss Of Function ◽  
Species Specific ◽  
And Function ◽  
The Impact

Abstract Codon usage frequency influences protein structure and function. The frequency with which codons are used potentially impacts primary, secondary and tertiary protein structure. Poor expression, loss of function, insolubility, or truncation can result from species-specific differences in codon usage. “Codon harmonization” more closely aligns native codon usage frequencies with those of the expression host particularly within putative inter-domain segments where slower rates of translation may play a role in protein folding. Heterologous expression of Plasmodium falciparum genes in Escherichia coli has been a challenge due to their AT-rich codon bias and the highly repetitive DNA sequences. Here, codon harmonization was applied to the malarial antigen, CelTOS (Cell-traversal protein for ookinetes and sporozoites). CelTOS is a highly conserved P. falciparum protein involved in cellular traversal through mosquito and vertebrate host cells. It reversibly refolds after thermal denaturation making it a desirable malarial vaccine candidate. Protein expressed in E. coli from a codon harmonized sequence of P. falciparum CelTOS (CH-PfCelTOS) was compared with protein expressed from the native codon sequence (N-PfCelTOS) to assess the impact of codon usage on protein expression levels, solubility, yield, stability, structural integrity, recognition with CelTOS-specific mAbs and immunogenicity in mice. While the translated proteins were expected to be identical, the translated products produced from the codon-harmonized sequence differed in helical content and showed a smaller distribution of polypeptides in mass spectra indicating lower heterogeneity of the codon harmonized version and fewer amino acid misincorporations. Substitutions of hydrophobic-to-hydrophobic amino acid were observed more commonly than any other. CH-PfCelTOS induced significantly higher antibody levels compared with N-PfCelTOS; however, no significant differences in either IFN-γ or IL-4 cellular responses were detected between the two antigens.

scholarly journals Site-specific replacement of amino acid residues within the CD binding loop of rat oncomodulin.

1990 ◽  
Vol 265 (24) ◽  
pp. 14450-14456 ◽  
Author(s):  
W.A. Palmisano ◽  
C.L. Treviño ◽  
M.T. Henzl
Keyword(s):  
Amino Acid ◽  
Amino Acid Residues ◽  
Site Specific ◽  
Binding Loop

scholarly journals Site-Specific Dynamics of Aβ1-23 Amyloid Formation and Fibrillar Configuration using an Unnatural Amino Acid

2015 ◽  
Vol 108 (2) ◽  
pp. 64a ◽  
Author(s):  
Deguo Du ◽  
Haiyang Liu ◽  
Richard Lantz ◽  
Patrick Cosme ◽  
Andrew C. Terentis ◽  
...  
Keyword(s):  
Amino Acid ◽  
Unnatural Amino Acid ◽  
Amyloid Formation ◽  
Site Specific

Effect of conserved intersubunit amino acid substitutions on Hfq protein structure and stability

2014 ◽  
Vol 79 (5) ◽  
pp. 469-477 ◽  
Author(s):  
V. N. Murina ◽  
B. S. Melnik ◽  
V. V. Filimonov ◽  
M. Ühlein ◽  
M. S. Weiss ◽  
...  
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Amino Acid Substitutions ◽  
Structure And Stability

scholarly journals The prediction of protein structure from amino acid sequence

1981 ◽  
Vol 37 (a1) ◽  
pp. C18-C18
Author(s):  
M. J. E. Sternberg ◽  
F. E. Cohen ◽  
W. R. Taylor
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Amino Acid Sequence
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