scholarly journals Label-Free “Digital Detection” of Single-Molecule DNA Hybridization with a Single Electron Transistor

2006 ◽  
Vol 128 (35) ◽  
pp. 11346-11347 ◽  
Author(s):  
Louis C. Brousseau
Keyword(s):  
Single Molecule ◽  
Dna Hybridization ◽  
Single Electron ◽  
Single Electron Transistor ◽  
Label Free ◽  
Digital Detection

scholarly journals Detecting Air Pollutant Molecules Using Tube-Shaped Single Electron Transistor

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7098
Author(s):  
Zhongkai Huang ◽  
Xiangyang Peng ◽  
Cheng Peng ◽  
Jin Huang ◽  
Maolin Bo ◽  
...  
Keyword(s):  
Single Molecule ◽  
Room Temperature ◽  
Air Pollutant ◽  
Single Electron ◽  
Single Electron Transistor ◽  
First Principles Calculations ◽  
Single Molecule Level ◽  
Stability Diagrams ◽  
Charge Stability ◽  
Axis Of Symmetry

An air pollution detector is proposed based on a tube-shaped single-electron transistor (SET) sensor. By monitoring the flow control component of the detector, each air pollutant molecule can be placed at the center of a SET nanopore and is treated as an island of the SET device in the same framework. Electron transport in the SET was incoherent, and the performances of the SET were sensitive at the single molecule level. Employing first-principles calculations, electronic features of an air pollutant molecule within a tube-shaped SET environment were found to be independent of the molecule rotational orientations with respect to axis of symmetry, unlike the electronic features in a conventional SET environment. Charge stability diagrams of the island molecules were demonstrated to be distinct for each molecule, and thus they can serve as electronic fingerprints for detection. Using the same setup, quantification of the air pollutant can be realized at room temperature as well. The results presented herein may help provide guidance for the identification and quantification of various types of air pollutants at the molecular level by treating the molecule as the island of the SET component in the proposed detector.

Single-molecule single-electron transistor (SM-SET) based on π-conjugated quinoidal-fused oligosilole and heteroepitaxial spherical Au/Pt nanogap electrodes

2019 ◽  
Vol 12 (12) ◽  
pp. 125007 ◽  
Author(s):  
Seung Joo Lee ◽  
Jaeyeon Kim ◽  
Tomohiro Tsuda ◽  
Ryo Takano ◽  
Ryo Shintani ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Electron ◽  
Single Electron Transistor ◽  
Nanogap Electrodes

scholarly journals Label-free single-molecule detection of DNA-hybridization kinetics with a carbon nanotube field-effect transistor

2011 ◽  
Vol 6 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Sebastian Sorgenfrei ◽  
Chien-yang Chiu ◽  
Ruben L. Gonzalez ◽  
Young-Jun Yu ◽  
Philip Kim ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Single Molecule ◽  
Dna Hybridization ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Single Molecule Detection ◽  
Label Free ◽  
Effect Transistor

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

Label-Free Electrochemical Detection of DNA Hybridization Related to Anthrax Lethal Factor by using Carbon Nanotube Modified Sensors

2019 ◽  
Vol 15 (4) ◽  
pp. 502-510 ◽  
Author(s):  
Hakan Karadeniz ◽  
Arzum Erdem
Keyword(s):  
Detection Limit ◽  
Dna Hybridization ◽  
Lethal Factor ◽  
Modified Electrodes ◽  
Label Free ◽  
Carbon Paste ◽  
Chip Technology ◽  
Anthrax Lethal Factor ◽  
Hybridization Time ◽  
Electrochemical Monitoring

Background: Anthrax Lethal Factor (ANT) is the dominant virulence factor produced by B. anthracis and is the major cause of death of infected animals. In this paper, pencil graphite electrodes GE were modified with single-walled and multi-walled carbon nanotubes (CNTs) for the detection of hybridization related to the ANT DNA for the first time in the literature. Methods: The electrochemical monitoring of label-free DNA hybridization related to ANT DNA was explored using both SCNT and MCNT modified PGEs with differential pulse voltammetry (DPV). The performance characteristics of ANT-DNA hybridization on disposable GEs were explored by measuring the guanine signal in terms of optimum analytical conditions; the concentration of SCNT and MCNT, the concentrations of probe and target, and also the hybridization time. Under the optimum conditions, the selectivity of probe modified electrodes was tested and the detection limit was calculated. Results: The selectivity of ANT probes immobilized onto MCNT-GEs was tested in the presence of hybridization of probe with NC no response was observed and with MM, smaller responses were observed in comparison to full-match DNA hybridization case. Even though there are unwanted substituents in the mixture samples containing both the target and NC in the ratio 1:1 and both the target and MM in the ratio 1:1, it has been found that ANT probe immobilized CNT modified graphite sensor can also select its target by resulting with 20.9% decreased response in comparison to the one measured in the case of full-match DNA hybridization case Therefore, it was concluded that the detection of direct DNA hybridization was performed by using MCNT-GEs with an acceptable selectivity. Conclusion: Disposable SCNT/MCNT modified GEs bring some important advantages to our assay including easy use, cost-effectiveness and giving a response in a shorter time compared to unmodified PGE, carbon paste electrode and glassy carbon electrode developed for electrochemical monitoring of DNA hybridization. Consequently, the detection of DNA hybridization related to the ANT DNA by MCNT modified sensors was performed by using lower CNT, probe and target concentrations, in a shorter hybridization time and resulting in a lower detection limit according to the SCNT modified sensors. In conclusion, MCNT modified sensors can yield the possibilities leading to the development of nucleic acid sensors platforms for the improvement of fast and cost-effective detection systems with respect to DNA chip technology.

Evidence for activated conduction in a single electron transistor

2001 ◽  
Vol 89 (1) ◽  
pp. 410-419 ◽  
Author(s):  
Nicole Y. Morgan ◽  
David Abusch-Magder ◽  
Marc A. Kastner ◽  
Yasuo Takahashi ◽  
Hiroyuki Tamura ◽  
...  
Keyword(s):  
Single Electron ◽  
Single Electron Transistor
Sign in / Sign up

Export Citation Format

Share Document