15N NMR Spin Relaxation Dispersion Study of the Molecular Crowding Effects on Protein Folding under Native Conditions

2006 ◽  
Vol 128 (12) ◽  
pp. 3916-3917 ◽  
Author(s):  
Xuanjun Ai ◽  
Zheng Zhou ◽  
Yawen Bai ◽  
Wing-Yiu Choy
Keyword(s):  
Protein Folding ◽  
Spin Relaxation ◽  
Relaxation Dispersion ◽  
15N Nmr ◽  
Molecular Crowding ◽  
Crowding Effects ◽  
Nmr Spin Relaxation

Two dimensional crowding effects on protein folding at interfaces observed by chiral vibrational sum frequency generation spectroscopy

2018 ◽  
Vol 20 (35) ◽  
pp. 22421-22426 ◽  
Author(s):  
Wei Liu ◽  
Li Fu ◽  
Zhuguang Wang ◽  
Zahra Sohrabpour ◽  
Xiaobai Li ◽  
...  
Keyword(s):  
Protein Folding ◽  
Sum Frequency Generation ◽  
Molecular Crowding ◽  
Two Dimensional ◽  
Sum Frequency ◽  
Crowding Effects ◽  
Sum Frequency Generation Spectroscopy ◽  
Frequency Generation

Vibrational sum frequency generation studies demonstrate the effects of two-dimensional molecular crowding on protein folding at interfaces.

Proton Spin Relaxation of a Liquid Crystal with a Glassy Cholesteric State

1990 ◽  
Vol 45 (9-10) ◽  
pp. 1077-1084 ◽  
Author(s):  
D. Pusiol ◽  
F. Noack ◽  
C. Aguilera
Keyword(s):  
Spin Relaxation ◽  
Rotational Diffusion ◽  
Glassy State ◽  
Larmor Frequency ◽  
Proton Spin ◽  
Relaxation Dispersion ◽  
Proton Relaxation ◽  
Relaxation Processes ◽  
Molecular Reorientation ◽  
Cholesteric Phase

Abstract Field-cycling and standard pulsed NMR techniques have been used to study the frequency dependence of the longitudinal proton spin relaxation time T x in the crystalline estradiol compound (+)3,1,7-ß-bis-(4n-butoxybenzoyloxy)-estra-1,3,5-(10)-trien or BET, which is a mesogenic material with a chiral molecular structure. From the measured Larmor frequency and temperature depen-dences we conclude that, at low NMR frequencies in the cholesteric phase, T1 reflects in addition to the relaxation process familiar from nematic liquid crystals (director fluctuation modes) another slow mechanism theoretically predicted for cholesteric systems, namely diffusion induced rotational molecular reorientation. These relaxation processes are not or much less effective in the crystalline and glassy state, where they are frozen. Also the high NMR frequency relaxation dispersion strongly differs between the cholesteric mesophase and the not liquid crystalline samples. This is interpreted by a change from essentially translational self-diffusion to rotational diffusion controlled proton relaxation.

Active site dynamics in NADH oxidase from Thermus thermophilus studied by NMR spin relaxation

2011 ◽  
Vol 51 (1-2) ◽  
pp. 71-82 ◽  
Author(s):  
Teresa Miletti ◽  
Patrick J. Farber ◽  
Anthony Mittermaier
Keyword(s):  
Spin Relaxation ◽  
Active Site ◽  
Nadh Oxidase ◽  
Thermus Thermophilus ◽  
Nmr Spin Relaxation
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