An Optical Biosensor for Rapid and Label-Free Detection of Cells

2006 ◽  
Vol 128 (12) ◽  
pp. 3862-3863 ◽  
Author(s):  
Ghanashyam Acharya ◽  
Chun-Li Chang ◽  
Cagri Savran
Keyword(s):  
Optical Biosensor ◽  
Label Free ◽  
Label Free Detection

scholarly journals Aptamer Functionalized Lipid Multilayer Gratings for Label-Free Analyte Detection

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2433
Author(s):  
Plengchart Prommapan ◽  
Nermina Brljak ◽  
Troy W. Lowry ◽  
David Van Winkle ◽  
Steven Lenhert
Keyword(s):  
Lipid Composition ◽  
Optical Biosensor ◽  
Optical Signal ◽  
Dna Aptamer ◽  
Critical Parameters ◽  
Label Free ◽  
Scale Thickness ◽  
Label Free Detection ◽  
Analyte Detection ◽  
Micrometer Scale

Lipid multilayer gratings are promising optical biosensor elements that are capable of transducing analyte binding events into changes in an optical signal. Unlike solid state transducers, reagents related to molecular recognition and signal amplification can be incorporated into the lipid grating ink volume prior to fabrication. Here we describe a strategy for functionalizing lipid multilayer gratings with a DNA aptamer for the protein thrombin that allows label-free analyte detection. A double cholesterol-tagged, double-stranded DNA linker was used to attach the aptamer to the lipid gratings. This approach was found to be sufficient for binding fluorescently labeled thrombin to lipid multilayers with micrometer-scale thickness. In order to achieve label-free detection with the sub-100 nm-thick lipid multilayer grating lines, the binding affinity was improved by varying the lipid composition. A colorimetric image analysis of the light diffracted from the gratings using a color camera was then used to identify the grating nanostructures that lead to an optimal signal. Lipid composition and multilayer thickness were found to be critical parameters for the signal transduction from the aptamer functionalized lipid multilayer gratings.

Development of a new parallelized, optical biosensor platform for label-free detection of autoimmunity-related antibodies

2013 ◽  
Vol 406 (14) ◽  
pp. 3305-3314 ◽  
Author(s):  
Oliver Bleher ◽  
Aline Schindler ◽  
Meng-Xin Yin ◽  
Andrew B. Holmes ◽  
Peter B. Luppa ◽  
...  
Keyword(s):  
Optical Biosensor ◽  
Label Free ◽  
Label Free Detection

scholarly journals Waveguiding and SERS Simplified Raman Spectroscopy on Biological Samples

Biosensors ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 37 ◽  
Author(s):  
Immanuel Valpapuram ◽  
Patrizio Candeloro ◽  
Maria Coluccio ◽  
Elvira Parrotta ◽  
Andrea Giugni ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Biosensor ◽  
Surface Enhanced Raman Spectroscopy ◽  
Limiting Factors ◽  
Laser Source ◽  
Label Free ◽  
Optical Signals ◽  
New Class ◽  
Label Free Detection ◽  
Surface Enhanced Raman

Biomarkers detection at an ultra-low concentration in biofluids (blood, serum, saliva, etc.) is a key point for the early diagnosis success and the development of personalized therapies. However, it remains a challenge due to limiting factors like (i) the complexity of analyzed media, and (ii) the aspecificity detection and the poor sensitivity of the conventional methods. In addition, several applications require the integration of the primary sensors with other devices (microfluidic devices, capillaries, flasks, vials, etc.) where transducing the signal might be difficult, reducing performances and applicability. In the present work, we demonstrate a new class of optical biosensor we have developed integrating an optical waveguide (OWG) with specific plasmonic surfaces. Exploiting the plasmonic resonance, the devices give consistent results in surface enhanced Raman spectroscopy (SERS) for continuous and label-free detection of biological compounds. The OWG allows driving optical signals in the proximity of SERS surfaces (detection area) overcoming spatial constraints, in order to reach places previously optically inaccessible. A rutile prism couples the remote laser source to the OWG, while a Raman spectrometer collects the SERS far field scattering. The present biosensors were implemented by a simple fabrication process, which includes photolithography and nanofabrication. By using such devices, it was possible to detect cell metabolites like Phenylalanine (Phe), Adenosine 5-triphosphate sodium hydrate (ATP), Sodium Lactate, Human Interleukin 6 (IL6), and relate them to possible metabolic pathway variation.

Label-Free Detection of Leptin Antibody-Antigen Interaction by Using LSPR-Based Optical Biosensor

2011 ◽  
Vol 11 (5) ◽  
pp. 4188-4193 ◽  
Author(s):  
Do-Kyun Kim ◽  
Tae Jung Park ◽  
Eiichi Tamiya ◽  
Sang Yup Lee
Keyword(s):  
Optical Biosensor ◽  
Label Free ◽  
Label Free Detection ◽  
Antigen Interaction

scholarly journals Integrated optical biosensor for rapid detection of bacteria

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Anna Mathesz ◽  
Sándor Valkai ◽  
Attila Újvárosy ◽  
Badri Aekbote ◽  
Orsolya Sipos ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Optical Biosensor ◽  
Medical Diagnostics ◽  
Microfluidic System ◽  
Body Fluids ◽  
Label Free ◽  
Label Free Detection ◽  
Integrated Optical ◽  
Escherichia Coli Bacteria

AbstractIn medical diagnostics, rapid detection of pathogenic bacteria from body fluids is one of the basic issues. Most state-of-the-art methods require optical labeling, increasing the complexity, duration and cost of the analysis. Therefore, there is a strong need for developing selective sensory devices based on label-free techniques, in order to increase the speed, and reduce the cost of detection. In a recent paper, we have shown that an integrated optical Mach-Zehnder interferometer, a highly sensitive all-optical device made of a cheap photopolymer, can be used as a powerful lab-on-a-chip tool for specific, labelfree detection of proteins. By proper modifications of this technique, our interferometric biosensor was combined with a microfluidic system allowing the rapid and specific detection of bacteria from solutions, having the surface of the sensor functionalized by bacterium-specific antibodies. The experiments proved that the biosensor was able to detect Escherichia coli bacteria at concentrations of 106 cfu/ml within a few minutes, that makes our device an appropriate tool for fast, label-free detection of bacteria from body fluids such as urine or sputum. On the other hand, possible applications of the device may not be restricted to medical microbiology, since bacterial identification is an important task in microbial forensics, criminal investigations, bio-terrorism threats and in environmental studies, as well.

Label-free detection of DNA hybridization using nanopillar arrays based optical biosensor

2014 ◽  
Vol 194 ◽  
pp. 10-18 ◽  
Author(s):  
Jem-Kun Chen ◽  
Gang-Yan Zhou ◽  
Chi-Jung Chang ◽  
Chih-Chia Cheng
Keyword(s):  
Dna Hybridization ◽  
Optical Biosensor ◽  
Label Free ◽  
Label Free Detection ◽  
Nanopillar Arrays

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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