A Columnar Phase of Dendritic Lipid−Based Cationic Liposome−DNA Complexes for Gene Delivery:  Hexagonally Ordered Cylindrical Micelles Embedded in a DNA Honeycomb Lattice

2006 ◽  
Vol 128 (12) ◽  
pp. 3998-4006 ◽  
Author(s):  
Kai K. Ewert ◽  
Heather M. Evans ◽  
Alexandra Zidovska ◽  
Nathan F. Bouxsein ◽  
Ayesha Ahmad ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Liposome ◽  
Honeycomb Lattice ◽  
Dna Complexes ◽  
Columnar Phase ◽  
Cylindrical Micelles ◽  
Hexagonally Ordered

Gene Delivery by Cationic Liposome-DNA Complexes

2001 ◽  
Author(s):  
Nejat Düzgünes¸ ◽  
Sérgio Simöes ◽  
Pedro Pires ◽  
Maria Pedroso de Lima
Keyword(s):  
Gene Delivery ◽  
Cationic Liposome ◽  
Dna Complexes

Gene Delivery by Cationic Liposome–DNA Complexes Containing Transferrin or Serum Albumin

2003 ◽  
pp. 369-383 ◽  
Author(s):  
Sérgio Simões ◽  
Pedro Pires ◽  
M.Teresa Girão da Cruz ◽  
Nejat Düzgüneş ◽  
Maria C Pedroso de Lima
Keyword(s):  
Gene Delivery ◽  
Serum Albumin ◽  
Cationic Liposome ◽  
Dna Complexes

Cationic liposome–DNA complexes: from liquid crystal science to gene delivery applications

2006 ◽  
Vol 364 (1847) ◽  
pp. 2573-2596 ◽  
Author(s):  
Cyrus R Safinya ◽  
Kai Ewert ◽  
Ayesha Ahmad ◽  
Heather M Evans ◽  
Uri Raviv ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Laser Scanning ◽  
Liquid Crystalline ◽  
Transfection Efficiency ◽  
Three Dimensional ◽  
Cationic Liposome ◽  
Laser Scanning Confocal Microscopy ◽  
Dna Complexes ◽  
Scanning Confocal Microscopy ◽  
Crystalline Nature

At present, there is an unprecedented level of interest in the properties and structures of complexes consisting of DNA mixed with oppositely charged cationic liposomes (CLs). The interest arises because the complexes mimic natural viruses as chemical carriers of DNA into cells in worldwide human gene therapy clinical trials. However, since our understanding of the mechanisms of action of CL–DNA complexes interacting with cells remains poor, significant additional insights and discoveries will be required before the development of efficient chemical carriers suitable for long-term therapeutic applications. Recent studies describe synchrotron X-ray diffraction, which has revealed the liquid crystalline nature of CL–DNA complexes, and three-dimensional laser-scanning confocal microscopy, which reveals CL–DNA pathways and interactions with cells. The importance of the liquid crystalline structures in biological function is revealed in the application of these modern techniques in combination with functional transfection efficiency measurements, which shows that the mechanism of gene release from complexes in the cell cytoplasm is dependent on their precise liquid crystalline nature and the physical and chemical parameters (for example, the membrane charge density) of the complexes. In §5 , we describe some recent new results aimed at developing bionanotube vectors for gene delivery.

Non-Viral Gene Delivery with Cationic Liposome–DNA Complexes

2008 ◽  
pp. 159-175 ◽  
Author(s):  
Kai K. Ewert ◽  
Ayesha Ahmad ◽  
Nathan F. Bouxsein ◽  
Heather M. Evans ◽  
Cyrus R. Safinya
Keyword(s):  
Gene Delivery ◽  
Cationic Liposome ◽  
Viral Gene ◽  
Dna Complexes ◽  
Viral Gene Delivery

Cationic liposome–DNA complexes as gene delivery vectors: Development and behaviour towards bone-like cells

2009 ◽  
Vol 5 (6) ◽  
pp. 2142-2151 ◽  
Author(s):  
A.C. Oliveira ◽  
M.P. Ferraz ◽  
F.J. Monteiro ◽  
S. Simões
Keyword(s):  
Gene Delivery ◽  
Cationic Liposome ◽  
Dna Complexes ◽  
Gene Delivery Vectors
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