De Novo Designed Peptidic Redox Potential Probe:  Linking Sensitized Emission to Disulfide Bond Formation

2004 ◽  
Vol 126 (42) ◽  
pp. 13616-13617 ◽  
Author(s):  
Kyung Lee ◽  
Valerie Dzubeck ◽  
Lauren Latshaw ◽  
Joel P. Schneider
Keyword(s):  
Redox Potential ◽  
Disulfide Bond ◽  
De Novo ◽  
Bond Formation ◽  
Disulfide Bond Formation

A Secreted Disulfide Catalyst Controls Extracellular Matrix Composition and Function

Science ◽  
2013 ◽  
Vol 341 (6141) ◽  
pp. 74-76 ◽  
Author(s):  
Tal Ilani ◽  
Assaf Alon ◽  
Iris Grossman ◽  
Ben Horowitz ◽  
Elena Kartvelishvily ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Disulfide Bond ◽  
De Novo ◽  
Bond Formation ◽  
Supporting Cell ◽  
Matrix Composition ◽  
Secretory Proteins ◽  
Disulfide Bond Formation ◽  
Enzyme Families ◽  
And Function

Disulfide bond formation in secretory proteins occurs primarily in the endoplasmic reticulum (ER), where multiple enzyme families catalyze cysteine cross-linking. Quiescin sulfhydryl oxidase 1 (QSOX1) is an atypical disulfide catalyst, localized to the Golgi apparatus or secreted from cells. We examined the physiological function for extracellular catalysis of de novo disulfide bond formation by QSOX1. QSOX1 activity was required for incorporation of laminin into the extracellular matrix (ECM) synthesized by fibroblasts, and ECM produced without QSOX1 was defective in supporting cell-matrix adhesion. We developed an inhibitory monoclonal antibody against QSOX1 that could modulate ECM properties and undermine cell migration.

scholarly journals DsbB Catalyzes Disulfide Bond Formation de Novo

2002 ◽  
Vol 277 (36) ◽  
pp. 32706-32713 ◽  
Author(s):  
James Regeimbal ◽  
James C. A. Bardwell
Keyword(s):  
Disulfide Bond ◽  
De Novo ◽  
Bond Formation ◽  
Disulfide Bond Formation ◽  
Formation De Novo

scholarly journals Oxygen-independent disulfide bond formation in VEGF-A and CA9

2021 ◽  
pp. 100505
Author(s):  
Fiana Levitin ◽  
Sandy Che-Eun Serena Lee ◽  
Stephanie Hulme ◽  
Ryan A. Rumantir ◽  
Amy S. Wong ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Bond Formation ◽  
Disulfide Bond Formation

scholarly journals The Sex Differences in Uveal Melanoma: Potential Roles of EIF1AX, Immune Response and Redox Regulation

2021 ◽  
Vol 28 (4) ◽  
pp. 2801-2811
Author(s):  
Feng Liu-Smith ◽  
Chi-Yang Chiu ◽  
Daniel L. Johnson ◽  
Phillip Winston Miller ◽  
Evan S. Glazer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Sex Difference ◽  
Uveal Melanoma ◽  
Disulfide Bond ◽  
Bond Formation ◽  
Disulfide Bond Formation ◽  
Men And Women ◽  
Copy Numbers ◽  
Differential Gene

Background: Uveal melanoma (UVM) is a rare cancer that shows sex difference in incidence and survival, with little previous report for the underlying mechanism. Methods: This study used the SEER data (1974–2016) for an age-dependent analysis on sex difference in UVM, and further used the TCGA-UVM genomics dataset for analyzing the differential gene expression profiles in tumors from men and women. Results: Our results demonstrate a sex difference in older age (≥40 years) but not in younger patients, with men exhibiting a higher incidence rate than women. However, younger women have shown a continuous increasing trend since 1974. Examining the 11 major oncogenes and tumor suppressors in UVM revealed that EIF1AX showed a significant sex difference in mRNA accumulation and copy number variation, with female tumors expressing higher levels of EIF1AX and exhibiting more variations in copy numbers. EIF1AX mRNA levels were significantly inversely correlated with EIF1AX copy numbers in female tumors only, but not in male tumors. Differential gene expression analysis at the whole genomic level identified a set of 92 protein-coding and 16 RNA-coding genes which exhibited differential expression in men and women (fold of change cutoff at 1.7, adjusted p value < 0.05, FDR < 0.05). Network analysis showed significant difference in immune response and in disulfide bond formation, with EGR1/EGR2 and PDIA2 genes as regulators for immune response and disulfide bond formation, respectively. The melanocortin pathway which is linked to both melanin synthesis and obesity seems to be altered with unclear significance, as the sex difference in POMC, DCT/TYRP2, and MRAP2 was observed but with no clear direction. Conclusion: This study reveals possible mechanisms for the sex difference in tumorigenesis of UVM which has potentials for better understanding and prevention of UVM.

Sign in / Sign up

Export Citation Format

Share Document