Nanomolar Protein Sensing with Embedded Receptor Molecules

2005 ◽  
Vol 127 (3) ◽  
pp. 904-915 ◽  
Author(s):  
Reza Zadmard ◽  
Thomas Schrader
Keyword(s):  
Protein Sensing

scholarly journals OBP14 (Odorant-Binding Protein) Sensing in Adelphocoris lineolatus Based on Peptide Nucleic Acid and Graphene Oxide

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 422
Author(s):  
Wenhua Tian ◽  
Tao Zhang ◽  
Shaohua Gu ◽  
Yuyuan Guo ◽  
Xiwu Gao ◽  
...  
Keyword(s):  
Peptide Nucleic Acid ◽  
Antennal Lobe ◽  
Behavioral Regulation ◽  
Dual Roles ◽  
Protein Sensing ◽  
Physiological Functions ◽  
Adelphocoris Lineolatus ◽  
Regulation Strategies ◽  
Odorant Binding

OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in A. lineolatus, the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on AlinOBP14, a Plus-C OBP from A. lineolatus, and we developed a PNA-GO-based mRNA biosensor to detect the expression of AlinOBP14. The results demonstrated that AlinOBP14 plays dual roles in A. lineolatus. The AlinOBP14 is expressed beneath the epidermis of the vertex and gena in heads of A. lineolatus, and it functions as a carrier for three terpenoids, while AlinOBP14 is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHⅢ. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest.

Surface Plasmon Resonance Enhanced Real-Time Photoelectrochemical Protein Sensing by Gold Nanoparticle-Decorated TiO2 Nanowires

2014 ◽  
Vol 86 (13) ◽  
pp. 6633-6639 ◽  
Author(s):  
Peimei Da ◽  
Wenjie Li ◽  
Xuan Lin ◽  
Yongcheng Wang ◽  
Jing Tang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Tio2 Nanowires ◽  
Protein Sensing

Hybrid Material for Protein Sensing Based on Electrosynthesized MIP on a Mannose Terminated Self-Assembled Monolayer

2013 ◽  
Vol 24 (15) ◽  
pp. 2233-2239 ◽  
Author(s):  
Decha Dechtrirat ◽  
Nenad Gajovic-Eichelmann ◽  
Frank F. Bier ◽  
Frieder W. Scheller
Keyword(s):  
Hybrid Material ◽  
Self Assembled Monolayer ◽  
Protein Sensing ◽  
Self Assembled

scholarly journals ATP compartmentation in plastids and cytosol ofArabidopsis thalianarevealed by fluorescent protein sensing

2018 ◽  
Vol 115 (45) ◽  
pp. E10778-E10787 ◽  
Author(s):  
Chia Pao Voon ◽  
Xiaoqian Guan ◽  
Yuzhe Sun ◽  
Abira Sahu ◽  
May Ngor Chan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Fluorescent Protein ◽  
Higher Plants ◽  
Electron Flow ◽  
Cyclic Electron Flow ◽  
Linear Electron ◽  
Protein Sensing ◽  
Atp Production ◽  
Respiratory Inhibitors ◽  
Sensor Protein

Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin–Benson–Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein expressed in livingArabidopsis thalianaseedlings, we found that MgATP2−concentrations were lower in the stroma of mature chloroplasts than in the cytosol, and exogenous ATP was able to enter chloroplasts isolated from 4- and 5-day-old seedlings, but not chloroplasts isolated from 10- or 20-day-old photosynthetic tissues. This observation is in line with the previous finding that the expression of chloroplast nucleotide transporters (NTTs) inArabidopsismesophyll is limited to very young seedlings. Employing a combination of photosynthetic and respiratory inhibitors with compartment-specific imaging of ATP, we corroborate the dependency of stromal ATP production on mitochondrial dissipation of photosynthetic reductant. Our data suggest that, during illumination, the provision and consumption of ATP:NADPH in chloroplasts can be balanced by exporting excess reductants rather than importing ATP from the cytosol.

M13 Bacteriophage as Materials for Amplified Surface Enhanced Raman Scattering Protein Sensing

2013 ◽  
Vol 24 (14) ◽  
pp. 2079-2084 ◽  
Author(s):  
Ju Hun Lee ◽  
Phyllis F. Xu ◽  
Dylan W. Domaille ◽  
Chulmin Choi ◽  
Sungho Jin ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Protein Sensing ◽  
M13 Bacteriophage ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Amino-acid, Peptide and Protein Sensing

2006 ◽  
pp. 287-309 ◽  
Author(s):  
Reza Zadmard ◽  
Thomas Schrader
Keyword(s):  
Amino Acid ◽  
Protein Sensing ◽  
Amino Acid Peptide

scholarly journals A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set

Sensors ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 5110
Author(s):  
Mari Okada ◽  
Hiroka Sugai ◽  
Shunsuke Tomita ◽  
Ryoji Kurita
Keyword(s):  
Pattern Recognition ◽  
Optical Response ◽  
Disease Diagnosis ◽  
Detection Methods ◽  
Fluorescence Signal ◽  
Signal Acquisition ◽  
Response Patterns ◽  
Protein Sensing ◽  
Composition Ratios ◽  
Protein Sensor

Recently, pattern-recognition-based protein sensing has received considerable attention because it offers unique opportunities that complement more conventional antibody-based detection methods. Here, we report a multichannel pattern-recognition-based sensor using a set of fluorophore-conjugated single-stranded DNAs (ssDNAs), which can detect various proteins. Three different fluorophore-conjugated ssDNAs were placed into a single microplate well together with a target protein, and the generated optical response pattern that corresponds to each environment-sensitive fluorophore was read via multiple detection channels. Multivariate analysis of the resulting optical response patterns allowed an accurate detection of eight different proteases, indicating that fluorescence signal acquisition from a single compartment containing a mixture of ssDNAs is an effective strategy for the characterization of the target proteins. Additionally, the sensor could identify proteins, which are potential targets for disease diagnosis, in a protease and inhibitor mixture of different composition ratios. As our sensor benefits from simple construction and measurement procedures, and uses accessible materials, it offers a rapid and simple platform for the detection of proteins.

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