Resonance Raman Imaging of the NADPH Oxidase Subunit Cytochromeb558in Single Neutrophilic Granulocytes

2003 ◽  
Vol 125 (40) ◽  
pp. 12112-12113 ◽  
Author(s):  
Henk-Jan van Manen ◽  
Natallia Uzunbajakava ◽  
Robin van Bruggen ◽  
Dirk Roos ◽  
Cees Otto
Keyword(s):  
Nadph Oxidase ◽  
Resonance Raman ◽  
Raman Imaging ◽  
Neutrophilic Granulocytes ◽  
Oxidase Subunit

Effects of red grape juice polyphenols in NADPH oxidase subunit expression in human neutrophils and mononuclear blood cells

2009 ◽  
Vol 102 (8) ◽  
pp. 1125-1135 ◽  
Author(s):  
Alberto Dávalos ◽  
Gema de la Peña ◽  
Carolina C. Sánchez-Martín ◽  
M. Teresa Guerra ◽  
Begoña Bartolomé ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Red Wine ◽  
Grape Juice ◽  
Cell Types ◽  
Human Neutrophils ◽  
Superoxide Anion Production ◽  
Oxidase Subunit ◽  
Subunit Expression ◽  
Red Grape Juice ◽  
Red Grape

The NADPH oxidase enzyme system is the main source of superoxide anions in phagocytic and vascular cells. NADPH oxidase-dependent superoxide generation has been found to be abnormally enhanced in several chronic diseases. Evidence is accumulating that polyphenols may have the potential to improve cardiovascular health, although the mechanism is not fully established. Consumption of concentrated red grape juice, rich in polyphenols, has been recently shown to reduce NADPH oxidase activity in circulating neutrophils from human subjects. In the present work we studied whether red grape juice polyphenols affected NADPH oxidase subunit expression at the transcription level. For this, we used human neutrophils and mononuclear cells from peripheral blood, HL-60-derived neutrophils and the endothelial cell line EA.hy926.Superoxide production was measured with 2′7′-dichlorofluorescein diacetate or lucigenin, mRNA expression by real-time RT-PCR and protein expression by Western blot. Each experiment was performed at least three times. In all cell types tested, red grape juice, dealcoholised red wine and pure polyphenols decreased superoxide anion production. Red grape juice and dealcoholised red wine selectively reduced p47phox, p22phox and gp91phox expression at both mRNA and protein levels, without affecting the expression of p67phox. Pure polyphenols, particularly quercetin, also reduced NADPH oxidase subunit expression, especially p47phox, in all cell types tested. The present results showing that red grape juice polyphenols reduce superoxide anion production provide an alternative mechanism by which consumption of grape derivatives may account for a reduction of oxidative stress associated with cardiovascular and/or inflammatory diseases related to NADPH oxidase superoxide overproduction.

NADPH oxidase subunit NOXO1 is a target for emphysema treatment in COPD

2020 ◽  
Vol 2 (6) ◽  
pp. 532-546
Author(s):  
Michael Seimetz ◽  
Natascha Sommer ◽  
Mariola Bednorz ◽  
Oleg Pak ◽  
Christine Veith ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Oxidase Subunit

scholarly journals Resonance Raman imaging of macular pigment distributions in the human retina

2008 ◽  
Vol 25 (4) ◽  
pp. 947 ◽  
Author(s):  
Mohsen Sharifzadeh ◽  
Da-You Zhao ◽  
Paul S. Bernstein ◽  
Werner Gellermann
Keyword(s):  
Resonance Raman ◽  
Macular Pigment ◽  
Raman Imaging ◽  
Human Retina

scholarly journals Hypoxia-Dependent Regulation of Nonphagocytic NADPH Oxidase Subunit NOX4 in the Pulmonary Vasculature

2007 ◽  
Vol 101 (3) ◽  
pp. 258-267 ◽  
Author(s):  
Manish Mittal ◽  
Markus Roth ◽  
Peter König ◽  
Simone Hofmann ◽  
Eva Dony ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Pulmonary Vasculature ◽  
Oxidase Subunit

scholarly journals The NADPH Oxidase Subunit p22 Inhibits the Function of the Tumor Suppressor Protein Tuberin

2010 ◽  
Vol 176 (5) ◽  
pp. 2447-2455 ◽  
Author(s):  
Karen Block ◽  
Yves Gorin ◽  
David D. New ◽  
Assaad Eid ◽  
Tomasz Chelmicki ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Nadph Oxidase ◽  
Tumor Suppressor Protein ◽  
Oxidase Subunit
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