Stable Conformations of Tripeptides in Aqueous Solution Studied by UV Circular Dichroism Spectroscopy

2003 ◽  
Vol 125 (27) ◽  
pp. 8178-8185 ◽  
Author(s):  
Fatma Eker ◽  
Kai Griebenow ◽  
Reinhard Schweitzer-Stenner
Keyword(s):  
Aqueous Solution ◽  
Circular Dichroism ◽  
Circular Dichroism Spectroscopy ◽  
Circular Dichroïsm

scholarly journals Conformation Transition Kinetics of Silk Fibroin in Aqueous Solution Explored Using Circular Dichroism Spectroscopy

2021 ◽  
Vol 6 (8) ◽  
pp. 1735-1740
Author(s):  
Sora Lee ◽  
Soo Hyun Kim ◽  
You‐Young Jo ◽  
Wan‐Taek Ju ◽  
Hyun‐Bok Kim ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Circular Dichroism ◽  
Silk Fibroin ◽  
Circular Dichroism Spectroscopy ◽  
Kinetics Of ◽  
Circular Dichroïsm ◽  
Conformation Transition

scholarly journals Physicochemical Characterisation of KEIF—The Intrinsically Disordered N-Terminal Region of Magnesium Transporter A

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 623
Author(s):  
Stéphanie Jephthah ◽  
Linda K. Månsson ◽  
Domagoj Belić ◽  
Jens Preben Morth ◽  
Marie Skepö
Keyword(s):  
Aqueous Solution ◽  
Circular Dichroism ◽  
Driving Forces ◽  
Circular Dichroism Spectroscopy ◽  
Terminal Region ◽  
Single Chain ◽  
Intrinsically Disordered ◽  
Magnesium Transporter ◽  
Dynamics Simulations ◽  
Circular Dichroïsm

Magnesium transporter A (MgtA) is an active transporter responsible for importing magnesium ions into the cytoplasm of prokaryotic cells. This study focuses on the peptide corresponding to the intrinsically disordered N-terminal region of MgtA, referred to as KEIF. Primary-structure and bioinformatic analyses were performed, followed by studies of the undisturbed single chain using a combination of techniques including small-angle X-ray scattering, circular dichroism spectroscopy, and atomistic molecular-dynamics simulations. Moreover, interactions with large unilamellar vesicles were investigated by using dynamic light scattering, laser Doppler velocimetry, cryogenic transmission electron microscopy, and circular dichroism spectroscopy. KEIF was confirmed to be intrinsically disordered in aqueous solution, although extended and containing little β-structure and possibly PPII structure. An increase of helical content was observed in organic solvent, and a similar effect was also seen in aqueous solution containing anionic vesicles. Interactions of cationic KEIF with anionic vesicles led to the hypothesis that KEIF adsorbs to the vesicle surface through electrostatic and entropic driving forces. Considering this, there is a possibility that the biological role of KEIF is to anchor MgtA in the cell membrane, although further investigation is needed to confirm this hypothesis.

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