Ile, Leu, and Val Methyl Assignments of the 723-Residue Malate Synthase G Using a New Labeling Strategy and Novel NMR Methods

2003 ◽  
Vol 125 (45) ◽  
pp. 13868-13878 ◽  
Author(s):  
Vitali Tugarinov ◽  
Lewis E. Kay
Keyword(s):  
Malate Synthase ◽  
Malate Synthase G ◽  
Nmr Methods

scholarly journals Biochemical characterization of malate synthase G of P. aeruginosa

2009 ◽  
Vol 10 (1) ◽  
pp. 20 ◽  
Author(s):  
Bart Roucourt ◽  
Nikki Minnebo ◽  
Patrick Augustijns ◽  
Kirsten Hertveldt ◽  
Guido Volckaert ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Malate Synthase ◽  
Malate Synthase G

TheglcBlocus ofRhizobium leguminosarumVF39 encodes an arabinose-inducible malate synthase

2002 ◽  
Vol 48 (10) ◽  
pp. 922-932 ◽  
Author(s):  
Alejandro García-de los Santos ◽  
Alejandro Morales ◽  
Laura Baldomá ◽  
Scott R.D Clark ◽  
Susana Brom ◽  
...  
Keyword(s):  
Sinorhizobium Meliloti ◽  
Rhizobium Leguminosarum ◽  
Plant Cell Wall ◽  
Glyoxylate Cycle ◽  
Malate Synthase ◽  
Mesorhizobium Loti ◽  
Malate Synthase G ◽  
Similar Genome Organization ◽  
The Glyoxylate Cycle

In the course of a study conducted to isolate genes upregulated by plant cell wall sugars, we identified an arabinose-inducible locus from a transcriptional fusion library of Rhizobium leguminosarum VF39, carrying random insertions of the lacZ transposon Tn5B22. Sequence analysis of the locus disrupted by the transposon revealed a high similarity to uncharacterized malate synthase G genes from Sinorhizobium meliloti, Agrobacterium tumefaciens, and Mesorhizobium loti. This enzyme catalyzes the condensation of glyoxylate and acetyl-CoA to yield malate and CoA and is thought to be a component of the glyoxylate cycle, which allows microorganisms to grow on two carbon compounds. Enzyme assays showed that a functional malate synthase is encoded in the glcB gene of R. leguminosarum and that its expression is induced by arabinose, glycolate, and glyoxylate. An Escherichia coli aceB glcB mutant, complemented with the R. leguminosarum PCR-amplified gene, recovered malate synthase activity. A very similar genome organization of the loci containing malate synthase and flanking genes was observed in R. leguminosarum, S. meliloti, and A. tumefaciens. Pea plants inoculated with the glcB mutant or the wild-type strain showed no significant differences in nitrogen fixation. This is the first report regarding the characterization of a mutant in one of the glyoxylate cycle enzymes in the rhizobia.Key words: Rhizobium, malate synthase, glyoxylate cycle, arabinose metabolism.

scholarly journals Structural and Functional Characterization of Malate Synthase G from Opportunistic Pathogen Pseudomonas aeruginosa

Biochemistry ◽  
2017 ◽  
Vol 56 (41) ◽  
pp. 5539-5549 ◽  
Author(s):  
Alyssa C. McVey ◽  
Prasanthi Medarametla ◽  
Xavier Chee ◽  
Sean Bartlett ◽  
Antti Poso ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Functional Characterization ◽  
Opportunistic Pathogen ◽  
Malate Synthase ◽  
Malate Synthase G
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