Effect of Light Scattering on Ultraviolet Difference Spectra1

1960 ◽  
Vol 82 (18) ◽  
pp. 4790-4792 ◽  
Author(s):  
S. J. Leach ◽  
H. A. Scheraga
Keyword(s):  
Light Scattering ◽  
Effect Of Light

scholarly journals A dual compartment cuvette system for correcting scattering in whole-cell absorbance spectroscopy of photosynthetic microorganisms

2021 ◽  
Author(s):  
John R. D. Hervey ◽  
Paolo Bombelli ◽  
David J. Lea-Smith ◽  
Alan K. Hulme ◽  
Nathan R. Hulme ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Light Scattering ◽  
Integrating Sphere ◽  
Whole Cell ◽  
Absorbance Spectroscopy ◽  
Dual Beam ◽  
Custom Made ◽  
Size And Morphology ◽  
Micro Organisms ◽  
Effect Of Light

AbstractAbsorption spectroscopy is widely used to determine absorption and transmission spectra of chromophores in solution, in addition to suspensions of particles, including micro-organisms. Light scattering, caused by photons deflected from part or all of the cells or other particles in suspension, results in distortions to the absorption spectra, lost information and poor resolution. A spectrophotometer with an integrating sphere may be used to alleviate this problem. However, these instruments are not universally available in biology laboratories, for reasons such as cost. Here, we describe a novel, rapid, and inexpensive technique that minimises the effect of light scattering when performing whole-cell spectroscopy. This method involves using a custom made dual compartment cuvette containing titanium dioxide in one chamber as a scattering agent. Measurements were conducted of a range of different photosynthetic micro-organisms of varying cell size and morphology, including cyanobacteria, eukaryotic microalgae and a purple non-sulphur bacterium. A concentration of 1 mg ml−1 titanium dioxide, using a spectrophotometer with a slit width of 5 nm, produced spectra for cyanobacteria and microalgae similar (1–4% difference) to those obtained using an integrating sphere. The spectrum > 520 nm was similar to that with an integrating sphere with the purple non-sulphur bacterium. This system produced superior results to those obtained using a recently reported method, the application of the diffusing agent, Scotch™ Magic tape, to the side of the cuvette. The protocol can be completed in an equivalent period of time to standard whole-cell absorbance spectroscopy techniques, and is, in principle, suitable for any dual-beam spectrophotometer.

The effect of light scattering on multifocal electroretinography

2002 ◽  
Vol 22 (6) ◽  
pp. 482-490 ◽  
Author(s):  
H. L. Chan ◽  
A. W. Siu ◽  
M. K. Yap ◽  
B. Brown
Keyword(s):  
Light Scattering ◽  
Multifocal Electroretinography ◽  
Effect Of Light

Effect of light scattering on infrared spectra

1972 ◽  
Vol 15 (11) ◽  
pp. 1691-1692
Author(s):  
T. P. Myasnikov ◽  
R. Ya. Evseeva
Keyword(s):  
Light Scattering ◽  
Infrared Spectra ◽  
Effect Of Light

Effect of Light Scattering by Optical Inhomogeneities in Ruby on Lasing Processes

1972 ◽  
Vol 7 (7) ◽  
pp. 845-850 ◽  
Author(s):  
M. S. Soskin ◽  
E. N. Sal'kova ◽  
P. P. Pogoretskii
Keyword(s):  
Light Scattering ◽  
Lasing Processes ◽  
Effect Of Light

Computational model of the effect of light scattering from cataracts in the human eye

2013 ◽  
Vol 30 (12) ◽  
pp. 2585 ◽  
Author(s):  
Ismael Kelly-Pérez ◽  
Neil C. Bruce ◽  
Luis R. Berriel-Valdos ◽  
Annette Werner ◽  
José A. Delgado Atencio
Keyword(s):  
Light Scattering ◽  
Computational Model ◽  
Human Eye ◽  
Effect Of Light

scholarly journals Effect of light scattering on optical-resolution photoacoustic microscopy

2012 ◽  
Author(s):  
Yan Liu ◽  
Chi Zhang ◽  
Song Hu ◽  
Yuta Suzuki ◽  
Zhun Xu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Optical Resolution ◽  
Photoacoustic Microscopy ◽  
Effect Of Light
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