Quantitative Determination of Amino Acids on Filter Paper Chromatograms by Direct Photometry

1949 ◽  
Vol 71 (12) ◽  
pp. 4121-4122 ◽  
Author(s):  
Louis B. Rockland ◽  
Max S. Dunn
Keyword(s):  
Amino Acids ◽  
Quantitative Determination ◽  
Filter Paper

QUANTITATIVE CHROMATOGRAPHIC METHODS: PART 5. AN IMPROVED NINHYDRIN–HYDRINDANTIN REAGENT

1961 ◽  
Vol 39 (2) ◽  
pp. 417-425 ◽  
Author(s):  
A. T. Matheson ◽  
E. Tigane ◽  
C. S. Hanes
Keyword(s):  
Amino Acids ◽  
Quantitative Determination ◽  
Filter Paper ◽  
High Stability ◽  
Reagent Blank ◽  
Chromatographic Methods ◽  
Ruhemann’S Purple

An improved ninhydrin–hydrindantin reagent has been developed for the quantitative determination of amino acids and peptides separated on filter paper chromatograms. This represents a modification of a reagent described earlier, which was based in turn upon the well-known solution of Moore and Stein. The present reagent has been in use for several years and has shown the advantages of high stability, extremely low and steady reagent blank values, and approximately stoichiometric yields of Ruhemann's purple for most amino acids. In addition to its use for determinations on excised areas from filter paper chromatograms, conditions are defined for the use of the reagent for determinations of amino acids in solutions.

scholarly journals Determination of Caffeine and Taurine Contents in Energy Drinks by HPLC-UV

2016 ◽  
Vol 9 ◽  
pp. 66-73 ◽  
Author(s):  
Krishna Prasad Rai ◽  
Hasta Bahadur Rai ◽  
Santosh Dahal ◽  
Saroj Chaudhary ◽  
Suraj Shrestha
Keyword(s):  
Amino Acids ◽  
Pilot Study ◽  
Quantitative Determination ◽  
Physiological Responses ◽  
Alcoholic Beverage ◽  
Energy Drinks ◽  
Energy Drink ◽  
Young Generation ◽  
Caffeine Content

Energy drinks are non-alcoholic beverage intended to enhance the psycho-physiological responses in human, which is especially popular among young generation in Nepal. It is normally high caffeinated drink added with other ingredients such as carbohydrates, amino acids, B-group of vitamins etc. In this study, 10 brands of energy drink available in Nepalese markets were taken then analyzed for quantitative determination of Caffeine and Taurine by HPLC-UV method. From the result obtained, pH and TSS values of energy drinks were found in the range of 2.96-3.81 and 6.64-18.21 respectively. Likewise, the Caffeine and Taurine content in same samples were found in the range of not detected (ND) to 35.78 mg/100 ml and ND to 387.5 mg/100 ml respectively. Only the 6 samples out of 10 were confi rmed caffeine content as per claimed in label, while only 3 samples were confi rmed for Taurine content as per label claimed. Based on this pilot study, the majority of samples did not meet the label claims in term of Caffeine and Taurine, which apparently indicated the misbranding of such drinks. Since, there is no any regulation for such energy drinks in Nepal, it seems to be a great challenge for regulation of their safety and misbranding.

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