Convenient Deuterium Labeling for Mass Spectrometry via Exchange of Enolizable Hydrogen on a Gas-Liquid Chromatography Column

1965 ◽  
Vol 87 (3) ◽  
pp. 680-681 ◽  
Author(s):  
Martin Senn ◽  
Wilhelm J. Richter ◽  
A. L. Burlingame
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Gas Liquid Chromatography ◽  
Deuterium Labeling ◽  
Chromatography Column

Sugar composition of ergot honeydews

1975 ◽  
Vol 53 (23) ◽  
pp. 2813-2825 ◽  
Author(s):  
R. L. Mower ◽  
J. G. Hancock
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Gel Filtration ◽  
Resonance Spectroscopy ◽  
Gas Liquid Chromatography ◽  
Deuterium Labeling ◽  
Sugar Composition ◽  
Methylation Analysis

Honeydews of several Claviceps spp., Sphacelia spp., and Sphacelia-like fungi were analyzed for differences in their sugar composition. Sugars were separated for comparative and preparative purposes by gel filtration and paper chromatography. The structures of pure sugars were determined with the use of periodate, enzymes, deuterium labeling, nuclear magnetic resonance spectroscopy, and methylation analysis by combined gas–liquid chromatography (glc) and mass spectrometry. Three sugars, 6 kestose, O-β-D-Fruf-(2 → 1)-O-β-D-Fruf-(2 → 6)-O-α-D-Glcp-(1 → 2)-β-D-Fruf, and O-β-D-Fruf-(2 → 6)-O-β-D-Fruf-(2 → 1)-O-α-D-Glcp-(6 → 2)-β-D-Fruf, not previously detected in ergot are reported.Sugar analysis of honeydews from infected hosts indicates that synthesis of the major sugars is controlled by the parasite and that sugar composition is distinct for each fungus. There were a number of taxonomic subgroupings that could be formed on the basis of honeydew constituents.

Concentrations of non-glucose polyols in serum and cerebrospinal fluid from apparently healthy adults and children.

1984 ◽  
Vol 30 (2) ◽  
pp. 188-191 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
S Seki ◽  
K Seki
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Healthy Subjects ◽  
Metabolic Diseases ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Adults And Children ◽  
Healthy Persons ◽  
Apparently Healthy

Abstract Six non-glucose polyols--mannose, fructose, 1-deoxyglucose, mannitol, glucitol, and inositol--were identified and evaluated in human serum and cerebrospinal fluid by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Concentrations of fructose, mannose, and inositol in the serum of healthy persons or children without metabolic diseases varied with age, as already reported for 1-deoxyglucose. Fructose, inositol, and glucitol concentrations in cerebrospinal fluid significantly exceeded those in serum. The method described here for determining polyols and for evaluating polyol patterns in serum, as well as the resulting data on children and healthy subjects, should be useful in investigations of the clinical and physiological significance of polyols.

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