Interactions of polyelectrolytes with simple electrolytes. III. The binding of magnesium ion by deoxyribonucleic acid

1968 ◽  
Vol 90 (12) ◽  
pp. 3081-3085 ◽  
Author(s):  
J. Skerjanc ◽  
U. P. Strauss
Keyword(s):  
Deoxyribonucleic Acid ◽  
Magnesium Ion

Extracellular nuclease produced by a marine bacterium. I. Extracellular deoxyribonuclease formation by a marine Vibrio sp.

1976 ◽  
Vol 22 (10) ◽  
pp. 1437-1442 ◽  
Author(s):  
M. Maeda ◽  
N. Taga
Keyword(s):  
Marine Bacterium ◽  
Deoxyribonucleic Acid ◽  
Culture Liquid ◽  
Dnase Activity ◽  
Magnesium Ion ◽  
Optimum Temperature ◽  
Deficient Medium ◽  
Marine Vibrio ◽  
Extracellular Nuclease ◽  
Marine Vibrio Sp

Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these organisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid – hydrolyzing activity. This organism requires salts of seawater for both growth and extracellular DNase formation. The DNase activity could not be detected in the synthetic seawater culture liquid lacking magnesium ion, and DNase activity decreased in a calcium-deficient medium. The optimum temperature for the growth of this organism was between 15 and 25 °C. The formation of extracellular DNase was the greatest at 20 °C and less activity was found at 10 and 30 °C.

MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

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