Mechanisms of reactions of thiosulfinates (sulfenic anhydrides). I. Thiosulfinate-sulfinic acid reaction

1967 ◽  
Vol 89 (14) ◽  
pp. 3557-3565 ◽  
Author(s):  
John L. Kice ◽  
Clifford G. Venier ◽  
Leslie. Heasley
Keyword(s):  
Sulfinic Acid ◽  
Acid Reaction ◽  
Mechanisms Of Reactions

The Mechanism of the Alkyl Sulfide—Sulfinic Acid Reaction. The Direction of Cleavage of Unsymmetrical Sulfides1a

1966 ◽  
Vol 31 (3) ◽  
pp. 848-852 ◽  
Author(s):  
John L. Kice ◽  
Bruce R. Toth ◽  
David C. Hampton ◽  
James F. Barbour
Keyword(s):  
Sulfinic Acid ◽  
Alkyl Sulfide ◽  
Acid Reaction

A Simple Method for Estimation of Lipoxygenase and Cyclo-Oxygenase Pathways in Human Platelets - the Use of Thiobarbituric Acid Reaction

1980 ◽  
Vol 44 (02) ◽  
pp. 111-114 ◽  
Author(s):  
Hiroshi Takayama ◽  
Minoru Okuma ◽  
Haruto Uchino
Keyword(s):  
Time Course ◽  
Normal Values ◽  
Human Platelet ◽  
Thiobarbituric Acid ◽  
Human Platelets ◽  
Optimal Conditions ◽  
Experimental Conditions ◽  
Simple Method ◽  
Acid Reaction ◽  
Optimal Ph

SummaryTo develop a simple method for estimation of platelet lipoxygenase (PLO) and cyclo-oxygenase (PCO) pathways, the arachidonic acid (AA) metabolism of human platelet was investigated under various experimental conditions by the use of the thiobarbituric acid (TBA) reaction and a radioisotope technique. A TBA-reactive substance different from malondialdehyde (MDA) via PCO pathway was detected and shown to be derived from the PLO pathway. Since the optimal pH and time course of its formation were different from those of MDA formation via PCO pathway, PLO and PCO pathways were estimated by quantitating the TBA-reactive substances produced by the incubation of AA either with aspirin-treated platelets or with untreated ones, respectively, each under optimal conditions. Normal values expressed in terms of nmol MDA/108 platelets were 1.17±0.34 (M±SD, n = 31) and 0.79±0.15 (n = 31) for PLO and PCO pathways, respectively.

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