Enzymic activity and .gamma.-phosphorus-32 labeling of fluorescent derivatives of cytidine triphosphate and adenosine triphosphate

1973 ◽  
Vol 95 (3) ◽  
pp. 961-962 ◽  
Author(s):  
Jorge R. Barrio ◽  
Laurence G. Dammann ◽  
Leslie H. Kirkegaard ◽  
Robert L. Switzer ◽  
Nelson J. Leonard
Keyword(s):  
Adenosine Triphosphate ◽  
Enzymic Activity ◽  
Derivatives Of ◽  
Fluorescent Derivatives

Synthesis of Fluorescent Derivatives of the Antibiotic Moenomycin A

2002 ◽  
Vol 2002 (7) ◽  
pp. 1149-1162 ◽  
Author(s):  
Andrij Buchynskyy ◽  
Uwe Kempin ◽  
Stefan Vogel ◽  
Lothar Hennig ◽  
Matthias Findeisen ◽  
...  
Keyword(s):  
Moenomycin A ◽  
Derivatives Of ◽  
Fluorescent Derivatives

Synthesis of fluorescent derivatives of wortmannin and demethoxyviridin as probes for phosphatidylinositol 3-kinase

2006 ◽  
Vol 16 (9) ◽  
pp. 2518-2521 ◽  
Author(s):  
José-Luis Giner ◽  
Karen A Kehbein ◽  
James A. Cook ◽  
Michele C. Smith ◽  
Chris J. Vlahos ◽  
...  
Keyword(s):  
Phosphatidylinositol 3 Kinase ◽  
Derivatives Of ◽  
Fluorescent Derivatives ◽  
Phosphatidylinositol 3

A fluorometric determination of sphingomyelinase by use of fluorescent derivatives of sphingomyelin, and its application to diagnosis of Niemann-Pick disease.

1980 ◽  
Vol 26 (1) ◽  
pp. 93-96 ◽  
Author(s):  
S Gatt ◽  
T Dinur ◽  
Y Barenholz
Keyword(s):  
Fatty Acyl ◽  
Great Sensitivity ◽  
Hair Follicles ◽  
Skin Fibroblasts ◽  
Acyl Residue ◽  
Niemann Pick Disease ◽  
Niemann Pick ◽  
Derivatives Of ◽  
Fluorescent Derivatives ◽  
Pick Disease

Abstract We synthesized fluorescent derivatives of sphingomyelin (N-acyl-sphingosylphosphocholine) and used them as substrates for several sphingomyelinases. The following five fluorescent probes, each attached to the terminal carbon atom of the fatty acyl residue, were introduced into sphingomyelin: dansyl, pyrene, carbazole, 4-chloro-7-nitrobenz-2-oxa-1,3-diazole, and anthroic acid. We compared the rates at which the fluoro- and radiolabeled sphingomyelins were hydrolyzed. They were the same with the following three spingomyelinases: (a) a purified enzyme from Staphylococcus aureus; (b) at Triton X100-treated extract of human brain (assayed at pH 7.4 in the presence of Mg2+; and (c) aqueous extracts of brain lysosomes, skin fibroblasts, and amniotic cells, assayed at pH 5.0. Homogenates of skin fibroblasts of a patient with Niemann-Pick disease had practically no activity when assayed at pH 5, with fluorosphingomyelin as substrate. When fluorosphingomyelin was mixed in various proportions with natural sphingomyelin, enzymes from each of the three sources hydrolyzed the two substrates at equal rates. The fluorosphingomyelins can be used to estimate with great sensitivity the sphingomyelinase activity in extracts of tissues or cells, in tears, and probably in hair follicles, as well as diagnose Niemann-Pick disease, either pre- or post-natally

scholarly journals Tight Binding of Bulky Fluorescent Derivatives of Adenosine to the Low Affinity E2ATP Site Leads to Inhibition of Na+/K+-ATPase

1999 ◽  
Vol 274 (4) ◽  
pp. 1971-1978 ◽  
Author(s):  
Detlef Thoenges ◽  
Evzen Amler ◽  
Thomas Eckert ◽  
Wilhelm Schoner
Keyword(s):  
Tight Binding ◽  
Derivatives Of ◽  
Fluorescent Derivatives
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