Ozonolysis of cis- and trans-1,2-difluoroethylene: isolation and characterization of trans-1,2-difluoroethylene oxide and cis,trans-1,2,3-trifluorocyclopropane

1982 ◽  
Vol 104 (3) ◽  
pp. 813-817 ◽  
Author(s):  
John W. Agopovich ◽  
Charles W. Gillies
Keyword(s):  
Isolation And Characterization ◽  
Cis And Trans

Synthesis, isolation, and characterization of the cis and trans isomers of steroidal 20-hydroxy-17(20)-en-21-aldehydes

1989 ◽  
Vol 54 (6) ◽  
pp. 1332-1338 ◽  
Author(s):  
Marvin L. Lewbart ◽  
Carl Monder ◽  
Walter J. Boyko ◽  
Carol J. Singer ◽  
F. Iohan
Keyword(s):  
Trans Isomers ◽  
Isolation And Characterization ◽  
Cis And Trans Isomers ◽  
Cis And Trans

Isolation and Characterization of the Photoalteration Products of cis- and trans-Chlordane

1975 ◽  
Vol 58 (1) ◽  
pp. 6-9
Author(s):  
Francis I Onuska ◽  
Michael E Comba
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Thin Layer ◽  
High Yield ◽  
Isolation And Characterization ◽  
The Individual ◽  
Cis And Trans ◽  
Layer Chromatography

Abstract Ultraviolet irradiation of cis- and trares-chlordane yielded 3 photolysis products. The expected half-caged analog of cis-chlordane was formed in high yield, and 2 minor photoproducts of trans-chlordane were observed. One of these products was a half-caged isomer. The individual photoproducts were isolated by thin layer chromatography and characterized by infrared, nuclear magnetic resonance, and mass spectrometry.

Isolation and characterization of a cDNA encoding a Δ8 sphingolipid desaturase from Aquilegia vulgaris

2002 ◽  
Vol 30 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
L. V. Michaelson ◽  
A. J. Longman ◽  
O. Sayanova ◽  
A. K. Stobart ◽  
J. A. Napier
Keyword(s):  
Functional Characterization ◽  
Yeast Cells ◽  
Sphingoid Bases ◽  
Yeast Saccharomyces Cerevisiae ◽  
Isolation And Characterization ◽  
Long Chain Base ◽  
In The Wild ◽  
Cis And Trans ◽  
Long Chain

We have isolated a cDNA encoding the Δ8 sphingolipid desaturase from the plant Aquilegia vulgaris L. via a PCR-based strategy using primers designed to target the conserved histidine box regions of microsomal desaturases. The function of the cDNA was confirmed by expression in the yeast, Saccharomyces cerevisiae. Analysis of the long-chain sphingoid bases as their dinitrophenyl derivatives by reverse-phase HPLC demonstrated the accumulation of cis- and trans-desaturated sphingoid bases which were not present in the wild-type yeast cells. The Δ8 desaturated products co-eluted with known Δ8-desaturated phytosphingenine and the molecular mass of these products was confirmed by liquid chromatography-MS. The Δ8 long-chain base desaturase was also able to desaturate dihydrosphingosine substrates. This is the first report of the functional characterization of an A. vulgaris gene product.

scholarly journals Bacterial and fungal oxidation of dibenzofuran

1979 ◽  
Vol 180 (1) ◽  
pp. 175-185 ◽  
Author(s):  
C E Cerniglia ◽  
J C Morgan ◽  
D T Gibson
Keyword(s):  
Cunninghamella Elegans ◽  
C Elegans ◽  
Isolation And Characterization ◽  
Cell Extracts ◽  
Cis And Trans Isomers ◽  
Relative Stereochemistry ◽  
Dihydrodiol Dehydrogenase ◽  
Bacteria And Fungi ◽  
Cis And Trans

Cunninghamella elegans and a mutant strain (B8/36) of Beijerinckia both oxidized dibenzofuran to 2,3-dihydroxy-2,3-dihydrodibenzofuran. The bacterial metabolite was extremely unstable and, in the presence of acid, was rapidly converted into a mixture of 2- and 3-hydroxydibenzofuran. In contrast, the 2,3-dihydroxy-2,3-dihydrodibenzofuran formed by C. elegans was stable and only yielded 2- and 3-hydroxydibenzofuran when heated under acidic conditions. The results suggest that Beijerinckia B8/36 and C. elegans form the respective cis- and trans-isomers of 2,3-dihydroxy-2,3-dihydrodibenzofuran. C. elegans also oxidized dibenzofuran to 2- and 3-hydroxydibenzofuran under conditions that would not lead to the dehydration of the trans-dihydrodiol. These observations implicate the initial formation of dibenzofuran- 2,3-epoxide in the fungal oxidation of dibenzofuran. Beijerinckia B8/36 also produced a second unstable dihydrodiol that was tentatively identified as cis-1,2-dihydroxy-1,2-dihydrodibenzofuran. This compound gave 2-hydroxydibenzofuran as the major dehydration product and the cis relative stereochemistry was suggested by the isolation and characterization of an isopropylidine derivative. A preparation of cis-naphthalene dihydrodiol dehydrogenase and cell extracts of the parent strain of Beijerinckia oxidized both bacterial dihydrodiols to catechols. Cell extracts prepared from C. elegans catalysed an analogous oxidation of trans-2,3-dihydroxy-2,3-dihydrodibenzofuran to 2,3-dihydroxydibenzofuran. The latter product was also isolated and identified from culture filtrates. The results suggest that bacteria and fungi utilize different mechanisms to initiate the oxidation of dibenzofuran.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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