Chlorine atom/benzene system. 1. The role of 6-chlorocyclohexadienyl radical

1986 ◽  
Vol 108 (20) ◽  
pp. 6300-6311 ◽  
Author(s):  
Philip S. Skell ◽  
Harry N. Baxter ◽  
James M. Tanko ◽  
Venkatasuryanarayana. Chebolu
Keyword(s):  
Author(s):  
Randy Moore

Previous work has indicated that the graft incompatihility between Sedrmi telephoides and Solanum pennellil involves cell necrosis that results In a thick layer of collapsed cells at the graft Interface. This necrotic layer insulates the stock from the scion, which results in abscission of the Sedum scion after 4-6 weeks due to desiccation and starvation. Thus, cell autolysis (which is restricted to Sedum) characterizes the Incompatibility response in this system (1). In order to elucidate the events that lead to cell autolysis, and thus better understand the cellular site and mode of action of cellular incompatibility, the appearance and fate of the hydrolytlc enzyme acid phosphatase (AP) was followed in both the compatible Sedum autograft and the incompatible Sedum/Solanum heterograft. Acid phosphatase was localized by a modified Gomori-type reaction; positive (i.e., including NaF inhibitor) and negative (lacking substrate) controls showed no enzymatic precipitate. Following an initial association with the endoplasmic reticulum (ER) and dictyosomes at 6-10 hours after grafting, AP activity in the compatible Sedum autograft is associated primarily with the plasmalemma (Fig. 1). By 18-24 hours after grafting, the AP activity is restricted to the tono-plast and vacuole (Fig. 2). This strict compartmentation and absence of enzyme from the cytosol is maintained throughout the development of the compatible graft. While AP activity in the incompatible Sedum/Solanum heterograft is Initially similar to the compatible Sedum autograft (i.e., initially found on the ER and dictyosomes), there is a marked difference in enzyme localization in the two graft partners as the incompatibility response develops. As in the compatible autograft, Solanum cells at the graft interface show an Increase in AP activity that Is restricted to the vacuole and tonoplast, with little or no enzyme activity in the cytosol (Fig. 3). In comparable Sedum cells, however, there is a dramatic Increase In AP activity in the cytosol (Fig. h); this cytosollc AP activity is associated with thin fibril-like structures (Fig. 5) measuring approximately 60 A in diameter. This high cytoplasmic AP activity In Sedum cells results in cell autolysis, death, and eventual cell collapse to form the characteristic necrotic layer separating the two graft partners.


2021 ◽  
Author(s):  
Olivier Bernard ◽  
Weiran Li ◽  
Fidel Costa ◽  
Caroline Bouvet de Maisonneuve

<p>One of the major challenges faced by volcanologists to investigate controls on eruption dynamics is to quantify both pre-eruptive volatile budgets and timescales of magma ascent. Indeed, petrological investigations of the two parameters usually rely on different methods/analytical techniques that are not always applicable/accessible. Recent studies have shown that the abundance and zoning pattern of F, Cl, and OH in apatite can be used to determine both pre-eruptive volatile budget and magma degassing rates that can, under some conditions, be related to magma ascent rates ([1],[2]).</p><p>Here we apply the two methods to apatite in the Rabaul 2006 eruption deposits (Papua-New-Guinea). This was a VEI-4 eruption and occurred in three main phases: (1) a sub-plinian onset followed 12h after its start by (2) a mixed strombolian-effusive phase, which subsequently evolved into (3) discrete vulcanian explosions. We sampled deposits of the three phases: (1) pumices, (2) fragments of lava flow, and (3) fragments of cow-pad bombs.</p><p>We calculated pre-eruptive water contents using apatite included in clinopyroxene as they keep a better record of reservoir conditions from the time of entrapment. We found that the magma that fed the sub-plinian phase contained the highest water content of about 2 wt.%, while magmas that fed the lava flow and the vulcanian phase were drier, with 0.2 to 0.5 wt.% less H<sub>2</sub>O. X-ray maps acquired with an EPMA show that only apatite crystals in the groundmass of the vulcanian and effusive deposits are zoned in F and Cl at the crystal rims, whereas those from the sub-plinian deposits and included in clinopyroxenes are not zoned. This indicates that the zoning is related to syn- or immediately pre-eruptive changes of Cl-F-H<sub>2</sub>O during magma ascent towards the surface and can thus be modelled as diffusive reequilibration of the crystal and the melt. We obtained maximum diffusion timescales of <8 hours for the unzoned apatite in sub-plinian deposits, timescales of 20–22 hours for apatite in vulcanian deposits, and 600–1500 hours for those in the lava flow. Thus, the time scales increase with decreasing explosivity of the eruptions, as it could be expected if magma ascent rate played the key role of eruption dynamics. However, the degassing timescales of the effusive phase are significantly longer than the eruption duration itself, which can be explained if the magma started rising in the system 1–3 months prior to the onset of the eruption. The volatile-rich, fast-rising magma that fed the initial sub-plinian phase propagated through, disturbed and remobilized the shallower, more degassed batch of magma, which was erupted during the following effusive phase. Deeper, volatile-poor magma that kept moving up the open conduit, was responsible for the late vulcanian explosions.</p><p>Our results show that apatite is a powerful tool for probing slight changes in magma volatile chemistry and ascent rates that can vary between different phases of the same eruption and produce different eruption styles.</p><p> </p><p>[1] Li and Costa, 2020, GCA [2] Li et al. 2020, EPSL</p>


2019 ◽  
pp. 145-161 ◽  
Author(s):  
Alyssa Joyce ◽  
Mike Timmons ◽  
Simon Goddek ◽  
Timea Pentz

AbstractThe growth rates and welfare of fish and the quality of plant production in aquaponics system rely on the composition and health of the system’s microbiota. The overall productivity depends on technical specifications for water quality and its movement amongst components of the system, including a wide range of parameters  including factors such as pH and flow rates which ensure that microbial components can act effectively in nitrification and remineralization processes. In this chapter, we explore current research examining the role of microbial communities in three units of an aquaponics system: (1) the recirculating aquaculture system (RAS) for fish production which includes biofiltration systems for denitrification; (2) the hydroponics units for plant production; and (3) biofilters and bioreactors, including sludge digester systems (SDS) involved in microbial decomposition and recovery/remineralization of solid wastes. In the various sub-disciplines related to each of these components, there is existing literature about microbial communities and their importance within each system (e.g. recirculating aquaculture systems (RAS), hydroponics, biofilters and digesters), but there is currently limited work examining interactions between these components in aquaponics system, thus making it an important area for further research.


Author(s):  
Seanna Hewitt ◽  
Amit Dhingra

Climacteric fruits are characterized by a dramatic increase in autocatalytic ethylene production, which is accompanied by a spike in respiration, at the onset of ripening. The change in the mode of ethylene production from autoinhibitory to auto-stimulatory is known as the system 1 (S1) to system 2 (S2) transition. Existing physiological models explain the basic and overarching genetic, hormonal, and transcriptional regulatory mechanisms governing the S1 to S2 transition of climacteric fruit. However, the links between ethylene and respiration, the two main factors that characterize the respiratory climacteric, have been largely understudied at the molecular level. Results of recent studies indicate that the AOX respiratory pathway may play an important role in mediating cross talk between ethylene response, carbon metabolism, ATP production, and ROS signaling during climacteric ripening. New genomic, metabolic, and epigenetic information sheds light on the interconnectedness of ripening-associated metabolic pathways, necessitating expanding the current, ethylene-centric physiological models. Understanding points at which ripening responses can be manipulated may reveal key, speciesand cultivar-specific targets for regulation of ripening enabling superior strategies for reducing postharvest wastage.


2020 ◽  
Author(s):  
Nick R. Elston ◽  
Michael Pablo ◽  
Fred Pimenta ◽  
Klaus M. Hahn ◽  
Takashi Watanabe

The small GTPases Rac1 and Rap1 can fulfill multiple cellular functions because their activation kinetics and localization are precisely controlled. To probe the role of their spatiotemporal dynamics, we generated optogenetic tools that activate or inhibit endogenous Rac and Rap1 in living cells. An improved version of the light induced dimerization (iLID) system [1] was used to control plasma membrane localization of protein domains that specifically activate or inactivate Rap1 and Rac (Tiam1 and Chimerin for Rac, RasGRP2 and Rap1GAP for Rap1 [2, 3, 4, 5]). Irradiation yielded a 50% to 230% increase in the concentration of these domains at the membrane, leading to effects on cell morphodynamics consistent with the known roles of Rac1 and Rap1.


Author(s):  
Ning Bai ◽  
Ya Gan ◽  
Xitong Li ◽  
Shuting Gao ◽  
Wenquan Yu ◽  
...  
Keyword(s):  

2016 ◽  
Vol 94 (4) ◽  
pp. 414-423 ◽  
Author(s):  
Levi H. Mielke ◽  
Amanda Furgeson ◽  
Charles A. Odame-Ankrah ◽  
Hans D. Osthoff

The role of nitryl chloride (ClNO2) as a nocturnal nitrogen oxide reservoir species and chlorine atom precursor is well established for polluted coastal areas, but its role at midcontinental locations is less clear. In this paper, intermittent measurements over the course of several seasons of ClNO2 mixing ratios by iodide ion chemical ionization mass spectrometry in Calgary, Alberta, Canada, are presented. Mixing ratios were highly variable between nights and seasons and depended on the abundances of precursors and meteorological conditions. The lowest ClNO2 mixing ratios (nocturnal maximum of 30 parts per trillion by volume (pptv)) were observed in the summer, rationalized by losses of the nitrate radical (NO3) that were more efficient than in the other months. Higher ClNO2 mixing ratios (up to 330 pptv) were observed in the winter and spring months but varied between nights. In the fall, ClNO2 mixing ratios increased from night to night following the application of salt to roads. The ClNO2 yield relative to the amount of NO3 produced from oxidation of NO2 by O3 ranged from 0.1% to 4.5% (10th and 90th percentiles, median 1.0%). The ClNO2 yield relative to N2O5 consumed by heterogeneous reactions was estimated using a time-integrated box model and ranged from 0.5% to 12.1% (10th and 90th percentiles, median 3.4%). The ubiquity of ClNO2 implies that the chlorine atom needs to be considered as an oxidant in high-latitude urban environments in winter.


2000 ◽  
Vol 325 (5-6) ◽  
pp. 693-697 ◽  
Author(s):  
P. Braña ◽  
B. Menéndez ◽  
T. Fernández ◽  
J.A. Sordo

1994 ◽  
Vol 358 ◽  
Author(s):  
R. Martins ◽  
M. Vieira ◽  
I. Ferreira ◽  
E. Fortunato

ABSTRACTThis work presents experimental data concerning the role of the oxygen partial pressure used during the preparation process, on the structure, composition and optoelectronic properties of wide band gap doped microcrystalline silicon oxycarbide films produced by a TCDDC system [1].


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