An analysis of the absorption and fluorescence spectra of trimethylamine. Determination of the ~A-~X origin and the ground state inversion barrier

1986 ◽  
Vol 108 (14) ◽  
pp. 3907-3912 ◽  
Author(s):  
Arthur M. Halpern ◽  
Mary Jo. Ondrechen ◽  
Lawrence D. Ziegler
Keyword(s):  
Ground State ◽  
Fluorescence Spectra ◽  
Absorption And Fluorescence Spectra ◽  
Inversion Barrier

Fluorescence Spectra and Determination of the Energy of O-O Transitions of Coumarins in Liquid Solutions

1990 ◽  
Vol 45 (5) ◽  
pp. 618-622 ◽  
Author(s):  
A. Bączyński ◽  
P. Targowski ◽  
B. Ziętek ◽  
D. Radomska
Keyword(s):  
Fluorescence Spectra ◽  
Room Temperature ◽  
Optical Transition ◽  
Solvent System ◽  
Absorption And Fluorescence Spectra ◽  
Initial State ◽  
Liquid Solutions ◽  
Analytical Expressions ◽  
Electronic Transition Energies

Abstract Analytical expressions of absorption and fluorescence spectra of some coumarins in ethyl alcohol at room temperature are given. Satisfactory agreement of measured and calculated spectra is obtained. It was possible to obtain the pure electronic transition energies immediately after excitation (from absorption spectra) and after the thermal and orientational equilibrium is reached (from fluorescence). An optical transition carries a dye-solvent system to a state in which the mutual interaction energy is larger than in the initial state

A Highly Resolved Yibronic Spectra of Dioxido-p-terphenyl and Trioxido-p-quaterphenyl in n-Hexanc at 77 K

1982 ◽  
Vol 37 (1) ◽  
pp. 91-94
Author(s):  
A. Kawski ◽  
M. Ligȩza
Keyword(s):  
Raman Spectra ◽  
Ground State ◽  
Fluorescence Spectra ◽  
Vibrational Structure ◽  
Ir And Raman Spectra ◽  
Absorption And Fluorescence Spectra ◽  
Excited Singlet ◽  
Ir And Raman ◽  
Good Agreement

Abstract Highly resolved vibronic absorption and fluorescence spectra of dioxido-p-terphenyl and the fluorescence spec-trum of trioxido-p-quaterphenyl in n-hexane at 77 K were obtained. The analysis of the vibrational structure in the excited singlet Si and in the ground So state gives funda-mental frequencies in the ground state in good agreement with those from infrared (IR) and Raman spectra.

scholarly journals Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Jing Kang ◽  
Oliver Kaczmarek ◽  
Jürgen Liebscher ◽  
Lars Dähne
Keyword(s):  
Quantitative Determination ◽  
Organic Solvents ◽  
Conformational Changes ◽  
Fluorescence Spectra ◽  
Cyanine Dye ◽  
Inhomogeneous Distribution ◽  
Aggregate Formation ◽  
Absorption And Fluorescence Spectra ◽  
Covalent Linkage

H-aggregates of the cyanine dye Cy5 are formed during covalent linkage to the cationic macromolecule Poly(allylamine) (PAH). The nonfluorescent H-aggregates strongly restrict the usage of the dye for analytical purposes and prevent a quantitative determination of the labeled macromolecules. The behavior of the H-aggregates has been studied by investigation of the absorption and fluorescence spectra of the dye polymer in dependence on solvent, label degree and additional sulfonate groups. H-aggregate formation is caused by an inhomogeneous distribution of the Cy5 molecules on the polymer chain. The H-aggregates can be destroyed by conformational changes of the PAH induced by interactions with polyanions or in organic solvents. It has been found that the polymer labeling process in high content of organic solvents can prevent the formation of H-aggregates. The results offer a better understanding and improvement of the use of the Cy5 dye for labeling purposes in fluorescence detection of macromolecules.

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