The tunichromes. A class of reducing blood pigments from sea squirts: isolation, structures, and vanadium chemistry

1988 ◽  
Vol 110 (18) ◽  
pp. 6162-6172 ◽  
Author(s):  
Eugene M. Oltz ◽  
Reimar C. Bruening ◽  
Mitchell J. Smith ◽  
Kenneth. Kustin ◽  
Koji. Nakanishi
Keyword(s):  
ChemInform ◽  
1988 ◽  
Vol 19 (51) ◽  
Author(s):  
E. M. OLTZ ◽  
R. C. BRUENING ◽  
M. J. SMITH ◽  
K. KUSTIN ◽  
K. NAKANISHI
Keyword(s):  

Author(s):  
Sergio A Muñoz-Gómez ◽  
Keira Durnin ◽  
Laura Eme ◽  
Christopher Paight ◽  
Christopher E Lane ◽  
...  

Abstract A most interesting exception within the parasitic Apicomplexa is Nephromyces, an extracellular, probably mutualistic, endosymbiont found living inside molgulid ascidian tunicates (i.e., sea squirts). Even though Nephromyces is now known to be an apicomplexan, many other questions about its nature remain unanswered. To gain further insights into the biology and evolutionary history of this unusual apicomplexan, we aimed to (1) find the precise phylogenetic position of Nephromyces within the Apicomplexa, (2) search for the apicoplast genome of Nephromyces, and (3) infer the major metabolic pathways in the apicoplast of Nephromyces. To do this, we sequenced a metagenome and a metatranscriptome from the molgulid renal sac, the specialized habitat where Nephromyces thrives. Our phylogenetic analyses of conserved nucleus-encoded genes robustly suggest that Nephromyces is a novel lineage sister to the Hematozoa, which comprises both the Haemosporidia (e.g., Plasmodium) and the Piroplasmida (e.g., Babesia and Theileria). Furthermore, a survey of the renal sac metagenome revealed 13 small contigs that closely resemble the genomes of the non-photosynthetic reduced plastids, or apicoplasts, of other apicomplexans. We show that these apicoplast genomes correspond to a diverse set of most closely related but genetically divergent Nephromyces lineages that co-inhabit a single tunicate host. In addition, the apicoplast of Nephromyces appears to have retained all biosynthetic pathways inferred to have been ancestral to parasitic apicomplexans. Our results shed light on the evolutionary history of the only probably mutualistic apicomplexan known, Nephromyces, and provide context for a better understanding of its life style and intricate symbiosis.


BMJ ◽  
1932 ◽  
Vol 1 (3706) ◽  
pp. 98-98 ◽  
Author(s):  
W. Marshall
Keyword(s):  

1950 ◽  
Vol 182 (3) ◽  
pp. 20-22 ◽  
Author(s):  
H. Munro Fox
Keyword(s):  

Nature ◽  
1943 ◽  
Vol 152 (3868) ◽  
pp. 714-716

1987 ◽  
Vol 33 (1) ◽  
pp. 132-134 ◽  
Author(s):  
S Bourdon ◽  
P Houzé ◽  
R Bourdon

Abstract A sensitive method, inductively coupled plasma atomic emission spectroscopy, is used to measure desferrioxamine in blood plasma. The desferrioxamine is transformed into its iron chelate, ferrioxamine, which is extracted into benzyl alcohol, then re-extracted into HCl (0.5 mol/L), which is used as the sample for the spectroscopy. For a 0.5-mL plasma sample, the detection limit (1 microgram/mL) suffices for following the concentration of desferrioxamine in plasma after its subcutaneous or intramuscular injection (40 mg per kg of body weight). Neither blood pigments nor trace metals interfere.


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