Protein Flexibility Correlates with Degree of Hydrogen Tunneling in Thermophilic and Mesophilic Alcohol Dehydrogenases

2000 ◽  
Vol 122 (43) ◽  
pp. 10738-10739 ◽  
Author(s):  
Amnon Kohen ◽  
Judith P. Klinman
Keyword(s):  
Protein Flexibility ◽  
Hydrogen Tunneling ◽  
Alcohol Dehydrogenases

Impact of Protein Flexibility on Hydride-Transfer Parameters in Thermophilic and Psychrophilic Alcohol Dehydrogenases

2004 ◽  
Vol 126 (31) ◽  
pp. 9500-9501 ◽  
Author(s):  
Zhao-Xun Liang ◽  
Iason Tsigos ◽  
Vassilis Bouriotis ◽  
Judith P. Klinman
Keyword(s):  
Hydride Transfer ◽  
Protein Flexibility ◽  
Alcohol Dehydrogenases ◽  
Transfer Parameters

scholarly journals Kinetic characterization of yeast alcohol dehydrogenases. Amino acid residue 294 and substrate specificity.

1987 ◽  
Vol 262 (8) ◽  
pp. 3754-3761
Author(s):  
A.J. Ganzhorn ◽  
D.W. Green ◽  
A.D. Hershey ◽  
R.M. Gould ◽  
B.V. Plapp
Keyword(s):  
Amino Acid ◽  
Substrate Specificity ◽  
Amino Acid Residue ◽  
Kinetic Characterization ◽  
Alcohol Dehydrogenases

scholarly journals THE ROLE OF ZINC IN ALCOHOL DEHYDROGENASES

1958 ◽  
Vol 232 (1) ◽  
pp. 453-464 ◽  
Author(s):  
Frederic L. Hoch ◽  
Robert J.P. Williams ◽  
Bert L. Vallee
Keyword(s):  
Alcohol Dehydrogenases

scholarly journals An ADH toolbox for raspberry ketone production from natural resources via a biocatalytic cascade

2021 ◽  
Author(s):  
Aileen Becker ◽  
Dominique Böttcher ◽  
Werner Katzer ◽  
Karsten Siems ◽  
Lutz Müller-Kuhrt ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Genetically Modified Organisms ◽  
Optical Purity ◽  
High Yield ◽  
Alcohol Dehydrogenases ◽  
Flavor Compound ◽  
Raspberry Ketone ◽  
Cofactor Recycling ◽  
Cosmetic Industry ◽  
Key Points

Abstract Raspberry ketone is a widely used flavor compound in food and cosmetic industry. Several processes for its biocatalytic production have already been described, but either with the use of genetically modified organisms (GMOs) or incomplete conversion of the variety of precursors that are available in nature. Such natural precursors are rhododendrol glycosides with different proportions of (R)- and (S)-rhododendrol depending on the origin. After hydrolysis of these rhododendrol glycosides, the formed rhododendrol enantiomers have to be oxidized to obtain the final product raspberry ketone. To be able to achieve a high conversion with different starting material, we assembled an alcohol dehydrogenase toolbox that can be accessed depending on the optical purity of the intermediate rhododendrol. This is demonstrated by converting racemic rhododendrol using a combination of (R)- and (S)-selective alcohol dehydrogenases together with a universal cofactor recycling system. Furthermore, we conducted a biocatalytic cascade reaction starting from naturally derived rhododendrol glycosides by the use of a glucosidase and an alcohol dehydrogenase to produce raspberry ketone in high yield. Key points • LB-ADH, LK-ADH and LS-ADH oxidize (R)-rhododendrol • RR-ADH and ADH1E oxidize (S)-rhododendrol • Raspberry ketone production via glucosidase and alcohol dehydrogenases from a toolbox Graphical abstract

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