UV endonuclease V from bacteriophage T4 catalyzes DNA strand cleavage at aldehydic abasic sites by a syn .beta.-elimination reaction

1989 ◽  
Vol 111 (20) ◽  
pp. 8029-8030 ◽  
Author(s):  
Abhijit Mazumder ◽  
John A. Gerlt ◽  
Lois Rabow ◽  
Michael J. Absalon ◽  
JoAnne Stubbe ◽  
...  
Keyword(s):  
Bacteriophage T4 ◽  
Elimination Reaction ◽  
Abasic Sites ◽  
Endonuclease V ◽  
Beta Elimination ◽  
Dna Strand

scholarly journals Bacteriophage-T4 and Micrococcus luteus UV endonucleases are not endonucleases but β-elimination and sometimes βδ-elimination catalysts

1989 ◽  
Vol 259 (3) ◽  
pp. 751-759 ◽  
Author(s):  
V Bailly ◽  
B Sente ◽  
W G Verly
Keyword(s):  
Enzyme Preparation ◽  
Micrococcus Luteus ◽  
Bacteriophage T4 ◽  
Pyrimidine Dimer ◽  
Elimination Reaction ◽  
Ap Sites ◽  
Beta Elimination ◽  
Ap Site ◽  
Polynucleotide Chain

Bacteriophage-T4 UV endonuclease nicks the C(3′)-O-P bond 3′ to AP (apurinic or apyrimidinic) sites by a beta-elimination reaction. The breakage of this bond is sometimes followed by the nicking of the C(5′)-O-P bond 5′ to the AP site, leaving a 3′-phosphate end; delta-elimination is proposed as a mechanism to explain this second reaction. The AP site formed when this enzyme acts on a pyrimidine dimer in a polynucleotide chain undergoes the same nicking reactions. Micrococcus luteus UV endonuclease also nicks the C(3′)-O-P bond 3′ to AP sites by a beta-elimination reaction. No subsequent delta-elimination was observed, but this might be due to the presence of 2-mercaptoethanol in the enzyme preparation.

scholarly journals Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase

1989 ◽  
Vol 262 (2) ◽  
pp. 581-589 ◽  
Author(s):  
V Bailly ◽  
W G Verly ◽  
T O'Connor ◽  
J Laval
Keyword(s):  
Escherichia Coli ◽  
Dna Glycosylase ◽  
Elimination Reaction ◽  
Phosphodiester Bonds ◽  
Beta Elimination ◽  
Dna Strand

Escherichia coli [formamidopyrimidine]DNA glycosylase catalyses the nicking of both the phosphodiester bonds 3′ and 5′ of apurinic or apyrimidinic sites in DNA so that the base-free deoxyribose is replaced by a gap limited by 3′-phosphate and 5′-phosphate ends. The two nickings are not the results of hydrolytic processes; the [formamidopyrimidine]DNA glycosylase rather catalyses a beta-elimination reaction that is immediately followed by a delta-elimination. The enzyme is without action on a 3′-terminal base-free deoxyribose or on a 3′-terminal base-free unsaturated sugar produced by a beta-elimination reaction nicking the DNA strand 3′ to an apurinic or apyrimidinic site.

Chemistry of Thermal Degradation of Abasic Sites in DNA. Mechanistic Investigation on Thermal DNA Strand Cleavage of Alkylated DNA

1994 ◽  
Vol 7 (5) ◽  
pp. 673-683 ◽  
Author(s):  
Hiroshi Sugiyama ◽  
Tsuyoshi Fujiwara ◽  
Atsushi Ura ◽  
Takahisa Tashiro ◽  
Koji Yamamoto ◽  
...  
Keyword(s):  
Thermal Degradation ◽  
Abasic Sites ◽  
Mechanistic Investigation ◽  
Dna Strand

A first-order base-initiated .beta.-elimination reaction involving a carbanion intermediate

1970 ◽  
Vol 92 (20) ◽  
pp. 5945-5949 ◽  
Author(s):  
Frederick G. Bordwell ◽  
Kwok-Chun. Yee ◽  
A. C. Knipe
Keyword(s):  
Elimination Reaction ◽  
First Order ◽  
Beta Elimination

ChemInform Abstract: THE BASE-INDUCED BETA-ELIMINATION REACTION OF 1,1,1-TRISUBSTITUTED HYDRAZINIUM SALTS

1974 ◽  
Vol 5 (37) ◽  
pp. no-no
Author(s):  
Y. TAMURA ◽  
J. MINAMIKAWA ◽  
O. NISHIKAWA ◽  
M. IKEDA
Keyword(s):  
Elimination Reaction ◽  
Beta Elimination ◽  
Hydrazinium Salts
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