Isopentenyl diphosphate isomerase. Mechanism of active-site-directed irreversible inhibition by 3-(fluoromethyl)-3-butenyl diphosphate

1989 ◽  
Vol 111 (10) ◽  
pp. 3740-3742 ◽  
Author(s):  
C. Dale Poulter ◽  
Manfred Muehlbacher ◽  
Darrell R. Davis
Keyword(s):  
Active Site ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Irreversible Inhibition

Isopentenyl-diphosphate isomerase: irreversible inhibition by 3-methyl-3,4-epoxybutyl diphosphate

Biochemistry ◽  
1992 ◽  
Vol 31 (41) ◽  
pp. 9955-9960 ◽  
Author(s):  
Xiang J. Lu ◽  
Dale J. Christensen ◽  
C. Dale Poulter
Keyword(s):  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Irreversible Inhibition

scholarly journals Biosynthesis of pinene in purple non-sulfur photosynthetic bacteria

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiaomin Wu ◽  
Guang Ma ◽  
Chuanyang Liu ◽  
Xin-yuan Qiu ◽  
Lu Min ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Heterologous Expression ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Bacteria ◽  
Fine Chemicals ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Geranyl Diphosphate ◽  
Ribosome Binding ◽  
Geranyl Diphosphate Synthase

Abstract Background Pinene is a monoterpene, that is used in the manufacture of fragrances, insecticide, fine chemicals, and renewable fuels. Production of pinene by metabolic-engineered microorganisms is a sustainable method. Purple non-sulfur photosynthetic bacteria belong to photosynthetic chassis that are widely used to synthesize natural chemicals. To date, researches on the synthesis of pinene by purple non-sulfur photosynthetic bacteria has not been reported, leaving the potential of purple non-sulfur photosynthetic bacteria synthesizing pinene unexplored. Results Rhodobacter sphaeroides strain was applied as a model and engineered to express the fusion protein of heterologous geranyl diphosphate synthase (GPPS) and pinene synthase (PS), hence achieving pinene production. The reaction condition of pinene production was optimized and 97.51 μg/L of pinene was yielded. Then, genes of 1-deoxy-d-xylulose 5-phosphate synthase, 1-deoxy-d-xylulose 5-phosphate reductoisomerase and isopentenyl diphosphate isomerase were overexpressed, and the ribosome binding site of GPPS-PS mRNA was optimized, improving pinene titer to 539.84 μg/L. Conclusions In this paper, through heterologous expression of GPPS-PS, pinene was successfully produced in R. sphaeroides, and pinene production was greatly improved by optimizing the expression of key enzymes. This is the first report on pinene produce by purple non-sulfur photosynthetic bacteria, which expands the availability of photosynthetic chassis for pinene production.

scholarly journals Crystal Structure of Type 2 Isopentenyl Diphosphate Isomerase fromThermus thermophilusin Complex with Inorganic Pyrophosphate†

Biochemistry ◽  
2008 ◽  
Vol 47 (35) ◽  
pp. 9051-9053 ◽  
Author(s):  
Jérôme de Ruyck ◽  
Jenny Pouyez ◽  
Steven C. Rothman ◽  
Dale Poulter ◽  
Johan Wouters
Keyword(s):  
Crystal Structure ◽  
Isopentenyl Diphosphate ◽  
Inorganic Pyrophosphate ◽  
Isopentenyl Diphosphate Isomerase

Exploring the Active Site ofTrypanosoma bruceiPhosphofructokinase by Inhibition Studies:  Specific Irreversible Inhibition†

Biochemistry ◽  
2002 ◽  
Vol 41 (32) ◽  
pp. 10183-10193 ◽  
Author(s):  
Samantha Claustre ◽  
Colette Denier ◽  
Faouzi Lakhdar-Ghazal ◽  
Andrée Lougare ◽  
Claudia Lopez ◽  
...  
Keyword(s):  
Active Site ◽  
Irreversible Inhibition ◽  
Inhibition Studies
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