3D Heteronuclear NMR techniques for carbon-13 in natural abundance

1990 ◽  
Vol 112 (23) ◽  
pp. 8599-8600 ◽  
Author(s):  
Horst Kessler ◽  
P. Schmieder ◽  
H. Oschkinat
Keyword(s):  
Heteronuclear Nmr ◽  
Natural Abundance ◽  
Nmr Techniques ◽  
3D Heteronuclear Nmr

Practical aspects of 3D heteronuclear NMR of proteins

1989 ◽  
Vol 84 (1) ◽  
pp. 72-84 ◽  
Author(s):  
Lewis E Kay ◽  
Dominique Marion ◽  
Ad Bax
Keyword(s):  
Heteronuclear Nmr ◽  
3D Heteronuclear Nmr

Introducing new half-integer quadrupolar nuclei for solid state NMR of inclusion compounds

2015 ◽  
Vol 93 (9) ◽  
pp. 1014-1024
Author(s):  
Igor L. Moudrakovski ◽  
Christopher I. Ratcliffe ◽  
John A. Ripmeester
Keyword(s):  
Solid State ◽  
Gas Hydrates ◽  
Inclusion Compounds ◽  
Chemical Shift Anisotropy ◽  
Natural Abundance ◽  
Quadrupolar Nuclei ◽  
Guest Molecules ◽  
Quadrupolar Coupling ◽  
Nmr Techniques ◽  
The Impact

Broad developments in experimental NMR techniques have opened new and exciting opportunities for application of solid state nuclear magnetic resonance (SS NMR) in studies of gas hydrates and inclusion compounds in general. Perhaps the most important advance of the last 10 years was the extension into very high magnetic fields beyond 20 T. This progress is especially significant in studies concerned with low-γ, low natural abundance, and quadrupolar nuclei. This work reports our recent exploration of clathrate hydrates and other inclusion compounds (β-quinol, tert-Bu-Calix[4], and dodecasil-3C) with SS NMR of nuclei that were not so long ago completely out of reach for NMR, namely 131Xe, 83Kr, and 33S. Although 129Xe is a widely used NMR probe, applications of the low-γ isotope 131Xe were very scarce. Being a quadrupolar spin 3/2 nucleus, 131Xe provides an additional probe for sampling the electric field gradients in inclusion compounds. Another nucleus that has been seriously under-explored is 83Kr, with its very low γ being the main obstacle, and along with quadrupolar coupling we report the first detection of the chemical shift anisotropy in krypton. The relative values of the Sternheimer antishielding factors for 131Xe and 83Kr, obtained by comparison of the spectra of the two in identical cage environments, are also discussed. Though 33S NMR of solids is notoriously difficult due to its low γ, low natural abundance, and relatively large quadrupolar moment, working at the field of 21.1 T it was possible to acquire, in a reasonable time, natural abundance 33S SS NMR spectra of various H2S and SO2 gas hydrates and inclusion compounds. In most cases the spectra are dominated by the quadrupolar interactions, providing information on the symmetry of the cages encapsulating the guest molecules, and also show the effects of very rapid reorientation of the encaged H2S and SO2. The impact of the introduction of new NMR nuclei on hydrate research is discussed.

Fast-pulsing NMR techniques for the detection of weak interactions: successful natural abundance probe of hydrogen bonds in peptides

2013 ◽  
Vol 11 (43) ◽  
pp. 7611 ◽  
Author(s):  
Amandine Altmayer-Henzien ◽  
Valérie Declerck ◽  
David J. Aitken ◽  
Ewen Lescop ◽  
Denis Merlet ◽  
...  
Keyword(s):  
Hydrogen Bonds ◽  
Weak Interactions ◽  
Natural Abundance ◽  
Nmr Techniques

Assignment of the protonated 13C resonances of apo-neocarzinostatin by 2D heteronuclear NMR spectroscopy at natural abundance

1994 ◽  
Vol 4 (5) ◽  
pp. 689-702 ◽  
Author(s):  
Claudine Lefevre ◽  
�lisabeth Adjadj ◽  
�ric Quiniou ◽  
Jo�l Mispelter
Keyword(s):  
Nmr Spectroscopy ◽  
Heteronuclear Nmr ◽  
Natural Abundance ◽  
Heteronuclear Nmr Spectroscopy

scholarly journals A New Polycyclic Polyprenylated Acylphloroglucinol Derivative from Garcinia verrucosa

2016 ◽  
Vol 11 (7) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Mialy Rajaonarivelo ◽  
Olga L. Rakotonandrasana ◽  
Marie-Thérèse Martin ◽  
Vincent Dumontet ◽  
Philippe Rasoanaivo
Keyword(s):  
Mass Spectrometry ◽  
Ethyl Acetate ◽  
Heteronuclear Nmr ◽  
Ethyl Acetate Extract ◽  
Screening Program ◽  
Stem Bark ◽  
Spectroscopic Methods ◽  
Phytochemical Screening ◽  
Nmr Techniques

In our continuing phytochemical screening program aimed at finding major constituents of endemic Madagascar plants as potential templates for semisynthesis, we investigated the ethyl acetate extract of stem bark of Garcinia verrucosa. Fractionation of the extract led to the isolation of the major compound named garcicosin. Its structure was elucidated by spectroscopic methods including 1D and 2D homo- and heteronuclear NMR techniques (COSY, HSQC, HMBC and NOESY), and HR-mass spectrometry.

Molecular structure and dynamics of proteins in solution: Insights derived from high-resolution NMR approaches

2003 ◽  
Vol 75 (10) ◽  
pp. 1371-1381 ◽  
Author(s):  
D. A. Torchia ◽  
Rieko Ishima
Keyword(s):  
Dipolar Coupling ◽  
Heteronuclear Nmr ◽  
Residual Dipolar Coupling ◽  
Structure And Dynamics ◽  
New Methods ◽  
Internal Motions ◽  
Relaxation Measurements ◽  
High Resolution Nmr ◽  
Nmr Techniques ◽  
Nuclear Overhauser

This article reviews heteronuclear NMR techniques that are used to characterize the 3D structures and internal molecular dynamics of proteins in solution. Applications to several proteins that have been studied in our laboratory are presented. Topics discussed include sequential assignments, the use of nuclear Overhauser enhancement spectroscopy (NOESY) and residual dipolar coupling restraints to determine protein structure, and the use of relaxation measurements to obtain information about protein internal motions. Also discussed are recent computational approaches directed at increasing the efficiency of structure determination, as well as new methods for studying large proteins.

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