2D and 3D NMR spectroscopy employing carbon-13/carbon-13 magnetization transfer by isotropic mixing. Spin system identification in large proteins

1990 ◽  
Vol 112 (2) ◽  
pp. 886-888 ◽  
Author(s):  
Stephen W. Fesik ◽  
Hugh L. Eaton ◽  
Edward T. Olejniczak ◽  
Erik R. P. Zuiderweg ◽  
Lawrence P. McIntosh ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
System Identification ◽  
Spin System ◽  
Magnetization Transfer ◽  
3D Nmr ◽  
Large Proteins ◽  
Isotropic Mixing ◽  
2D And 3D

scholarly journals Structure of Nanobody Nb23

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3567
Author(s):  
Mathias Percipalle ◽  
Yamanappa Hunashal ◽  
Jan Steyaert ◽  
Federico Fogolari ◽  
Gennaro Esposito
Keyword(s):  
Nmr Spectroscopy ◽  
Chemical Shift ◽  
Solution Structure ◽  
Chemical Shifts ◽  
Molecular Size ◽  
Side Chain ◽  
3D Nmr ◽  
Target Antigen ◽  
Internuclear Distances ◽  
2D And 3D

Background: Nanobodies, or VHHs, are derived from heavy chain-only antibodies (hcAbs) found in camelids. They overcome some of the inherent limitations of monoclonal antibodies (mAbs) and derivatives thereof, due to their smaller molecular size and higher stability, and thus present an alternative to mAbs for therapeutic use. Two nanobodies, Nb23 and Nb24, have been shown to similarly inhibit the self-aggregation of very amyloidogenic variants of β2-microglobulin. Here, the structure of Nb23 was modeled with the Chemical-Shift (CS)-Rosetta server using chemical shift assignments from nuclear magnetic resonance (NMR) spectroscopy experiments, and used as prior knowledge in PONDEROSA restrained modeling based on experimentally assessed internuclear distances. Further validation was comparatively obtained with the results of molecular dynamics trajectories calculated from the resulting best energy-minimized Nb23 conformers. Methods: 2D and 3D NMR spectroscopy experiments were carried out to determine the assignment of the backbone and side chain hydrogen, nitrogen and carbon resonances to extract chemical shifts and interproton separations for restrained modeling. Results: The solution structure of isolated Nb23 nanobody was determined. Conclusions: The structural analysis indicated that isolated Nb23 has a dynamic CDR3 loop distributed over different orientations with respect to Nb24, which could determine differences in target antigen affinity or complex lability.

Homonuclear three-dimensional proton NMR spectroscopy of pike parvalbumin. Comparison of short- and medium-range NOEs from 2D and 3D NMR

1990 ◽  
Vol 112 (13) ◽  
pp. 5024-5030 ◽  
Author(s):  
Andre Padilla ◽  
Geerten W. Vuister ◽  
Rolf Boelens ◽  
Gerard J. Kleywegt ◽  
Adrien Cave ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Three Dimensional ◽  
Proton Nmr ◽  
3D Nmr ◽  
Proton Nmr Spectroscopy ◽  
Medium Range ◽  
2D And 3D

Solvent suppression using a spin lock in 2D and 3D NMR spectroscopy with H2O solutions

1989 ◽  
Vol 85 (3) ◽  
pp. 608-613 ◽  
Author(s):  
Barbara A Messerle ◽  
Gerhard Wider ◽  
Gottfried Otting ◽  
Christoph Weber ◽  
Kurt Wüthrich
Keyword(s):  
Nmr Spectroscopy ◽  
3D Nmr ◽  
Spin Lock ◽  
Solvent Suppression ◽  
Lock In ◽  
2D And 3D

Solvent Suppression Using a Spin Lock in 2D and 3D NMR Spectroscopy with H2O Solutions

1995 ◽  
pp. 411-416
Author(s):  
BARBARA A. MESSERLE ◽  
GERHARD WIDER ◽  
GOTTFRIED OTTING ◽  
CHRISTOPH WEBER ◽  
KURT WÜTHRICH
Keyword(s):  
Nmr Spectroscopy ◽  
3D Nmr ◽  
Spin Lock ◽  
Solvent Suppression ◽  
Lock In ◽  
2D And 3D

Multiphoton NMR spectroscopy on a spin system withI=1/2

1983 ◽  
Vol 78 (9) ◽  
pp. 5293-5310 ◽  
Author(s):  
Y. Zur ◽  
M. H. Levitt ◽  
S. Vega
Keyword(s):  
Nmr Spectroscopy ◽  
Spin System

Local symmetry in 2D and 3D NMR spectra

1989 ◽  
Vol 85 (2) ◽  
pp. 337-358 ◽  
Author(s):  
S Boentges ◽  
B.U Meier ◽  
C Griesinger ◽  
R.R Ernst
Keyword(s):  
Nmr Spectra ◽  
Local Symmetry ◽  
3D Nmr ◽  
2D And 3D
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