scholarly journals Enzyme Mimic Displaying Oscillatory Behavior. Oscillating Reduction of Manganese(III) Porphyrin in a Membrane-Bound Cytochrome P-450 Model System

1995 ◽  
Vol 117 (50) ◽  
pp. 12655-12656 ◽  
Author(s):  
Albertus Schenning ◽  
Jeffrey Lutje Spelberg ◽  
Martijn Driessen ◽  
Marcus Hauser ◽  
Martinus Feiters ◽  
...  
Keyword(s):  
Enzyme Mimic ◽  
Model System ◽  
Oscillatory Behavior ◽  
Membrane Bound ◽  
Cytochrome P 450

A combination of 5-fluorouracil and membrane-bound antibody inhibits B-cell lymphoma growth in a mouse model system

2007 ◽  
Vol 48 (2) ◽  
pp. 406-409 ◽  
Author(s):  
Mattia Bonsignori ◽  
Sybil D'Costa ◽  
Sherri Surman ◽  
Julia L. Hurwitz
Keyword(s):  
Mouse Model ◽  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Model System ◽  
Membrane Bound ◽  
Mouse Model System

Ductus arteriosus: involvement of a sarcolemmal cytochrome P-450 in O2 constriction?

1989 ◽  
Vol 67 (11) ◽  
pp. 1448-1450 ◽  
Author(s):  
Flavio Coceani ◽  
Julie Wright ◽  
Carole Breen
Keyword(s):  
Carbon Monoxide ◽  
Ductus Arteriosus ◽  
Signal Transducer ◽  
Mixture Ratio ◽  
Skinned Muscle ◽  
Sequence Of Events ◽  
Membrane Bound ◽  
Cytochrome P 450 ◽  
Constrictor Response ◽  
Insight Into

Our previous studies implicate a cytochrome P-450-based mechanism in the constrictor response of the ductus arteriosus to oxygen. The present experiments were conducted on saponin-skinned strips of ductal muscle from mature fetal lambs to determine the location, sarcolemmal versus intracellular, of this cytochrome and to obtain a better insight into the sequence of events underlying the action of oxygen. Skinned preparations contracted to free Ca2+ over the range between 0.1 and 5–10 μM (pCa 7 to 5). In contrast, oxygen (Po2, 608–690 Torr; 1 Torr = 133.3 Pa) had no significant effect, both in the absence and presence of 10 μM calcium. Carbon monoxide, tested as pure CO or a CO–O2 mixture (ratio 0.28), did not relax preparations maximally contracted with calcium. These findings indicate that oxygen exerts its effect on the plasma membrane of ductus muscle cells and that a membrane-bound cytochrome P-450 mechanism likely functions as the signal transducer for oxygen in the formation of a constrictor agent.Key words: ductus arteriosus closure, chemically skinned muscle, second messenger.

Video microscopic studies of membrane dynamics

1991 ◽  
Vol 49 ◽  
pp. 232-233
Author(s):  
Ken Jacobson ◽  
Greta Lee ◽  
Juliet Lee
Keyword(s):  
Living Cells ◽  
Model System ◽  
Random Motion ◽  
Membrane Dynamics ◽  
Two Dimensional ◽  
Planar Bilayer ◽  
Microscopic Studies ◽  
Membrane Bound ◽  
Random Patterns ◽  
Membrane Components

The application of several imaging technologies and photobleaching to problems in membrane dynamics will be presented. These techniques emphasize different aspects of the measurement of translational transport: (1) tracing the trajectories of individual molecules undergoing a two dimensional random walk, and (2) investigating bulk changes in the distribution of membrane components in the plane of the membrane in single, locomoting cells.Nanovid microscopy, which uses 30-40 nm colloidal gold probes combined with video-enhanced contrast, allows investigation of random and directed movements of individual molecules in artificial membranes or in the plasma membrane of living cells. The movements of lipid molecules were followed in a supported, planar bilayer containing fluorescein-phosphatidylethanolamine (Fl-PE) labelled with 30 ran gold anti-fluorescein (anti-Fl) to validate the technique in a model system. The membrane-bound gold particles moved in random patterns which were indistinguishable from those produced by computer simulations of two dimensional random motion.

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