Partial reversal of the substrate stereospecificity of an L-lactate dehydrogenase by site-directed mutagenesis

Roman Sakowicz; Marvin Gold; J. Bryan Jones

doi:10.1021/ja00114a002

Partial reversal of the substrate stereospecificity of an L-lactate dehydrogenase by site-directed mutagenesis

Journal of the American Chemical Society ◽

10.1021/ja00114a002 ◽

1995 ◽

Vol 117 (9) ◽

pp. 2387-2394 ◽

Cited By ~ 17

Author(s):

Roman Sakowicz ◽

Marvin Gold ◽

J. Bryan Jones

Keyword(s):

Lactate Dehydrogenase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Partial Reversal

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Site-directed mutagenesis of Bacillus stearothermophilus lactate dehydrogenase

Biochemical Society Transactions ◽

10.1042/bst0150152 ◽

1987 ◽

Vol 15 (1) ◽

pp. 152-153 ◽

Cited By ~ 3

Author(s):

ANTHONY R. CLARKE ◽

DALE B. WIGLEY ◽

DAVID A. BARSTOW ◽

WILLIAM N. CHIA ◽

ADAM D. B. WALDMAN ◽

...

Keyword(s):

Lactate Dehydrogenase ◽

Bacillus Stearothermophilus ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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An evaluation of the substrate specificity, and of its modification by site-directed mutagenesis, of the cloned L-lactate dehydrogenase from Bacillus stearothermophilus

Journal of the American Chemical Society ◽

10.1021/ja00199a046 ◽

1989 ◽

Vol 111 (17) ◽

pp. 6800-6804 ◽

Cited By ~ 28

Author(s):

Marcel A. Luyten ◽

Daniel Bur ◽

Hla Wynn ◽

Wendy Parris ◽

Marvin Gold ◽

...

Keyword(s):

Lactate Dehydrogenase ◽

Substrate Specificity ◽

Bacillus Stearothermophilus ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Attempting to remove the substrate inhibition ofL-lactate dehydrogenase fromBacillus stearothermophilus by site-directed mutagenesis

Applied Biochemistry and Biotechnology ◽

10.1007/bf02729067 ◽

2007 ◽

Vol 141 (2-3) ◽

pp. 265-272 ◽

Cited By ~ 9

Author(s):

Bariş Binay ◽

Nevin Gül Karagüler

Keyword(s):

Lactate Dehydrogenase ◽

Substrate Inhibition ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Enhancement of the catalytic activity of d-lactate dehydrogenase from Sporolactobacillus laevolacticus by site-directed mutagenesis

Biochemical Engineering Journal ◽

10.1016/j.bej.2018.02.015 ◽

2018 ◽

Vol 133 ◽

pp. 214-218

Author(s):

Kento Nakano ◽

Shoichi Sawada ◽

Ryosuke Yamada ◽

Takashi Mimitsuka ◽

Hiroyasu Ogino

Keyword(s):

Catalytic Activity ◽

Lactate Dehydrogenase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Analysis of active site loop amino acids of lactate dehydrogenase fromPlasmodium vivaxby site-directed mutagenesis studies

Drug Development Research ◽

10.1002/ddr.20089 ◽

2006 ◽

Vol 67 (2) ◽

pp. 175-180 ◽

Cited By ~ 5

Author(s):

Dilek Turgut-Balik ◽

Dilek Sadak ◽

Venhar Celik

Keyword(s):

Amino Acids ◽

Lactate Dehydrogenase ◽

Active Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Functional and structural characterization of the pentapeptide insertion of Theileria annulata lactate dehydrogenase by site-directed mutagenesis, comparative modeling and molecular dynamics simulations

Journal of Molecular Graphics and Modelling ◽

10.1016/j.jmgm.2017.04.010 ◽

2017 ◽

Vol 74 ◽

pp. 344-351 ◽

Cited By ~ 1

Author(s):

Aysegul Erdemir ◽

Ozal Mutlu

Keyword(s):

Molecular Dynamics ◽

Lactate Dehydrogenase ◽

Molecular Dynamics Simulations ◽

Structural Characterization ◽

Comparative Modeling ◽

Theileria Annulata ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Dynamics Simulations

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Redesign of the coenzyme specificity in l-Lactate dehydrogenase from Bacillus stearothermophilus using site-directed mutagenesis and media engineering

Protein Engineering Design and Selection ◽

10.1093/protein/12.10.851 ◽

1999 ◽

Vol 12 (10) ◽

pp. 851-856 ◽

Cited By ~ 59

Author(s):

Niklas Holmberg ◽

Ulf Ryde ◽

Leif Bülow

Keyword(s):

Lactate Dehydrogenase ◽

Bacillus Stearothermophilus ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Coenzyme Specificity

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Site-directed mutagenesis reveals role of mobile arginine residue in lactate dehydrogenase catalysis

Nature ◽

10.1038/324699a0 ◽

1986 ◽

Vol 324 (6098) ◽

pp. 699-702 ◽

Cited By ~ 122

Author(s):

Anthony R. Clarke ◽

Dale B. Wigley ◽

William N. Chia ◽

David Barstow ◽

Tony Atkinson ◽

...

Keyword(s):

Lactate Dehydrogenase ◽

Site Directed Mutagenesis ◽

Arginine Residue ◽

Directed Mutagenesis

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Improved specificity toward substrates with positively charged side chains by site-directed mutagenesis of the L-lactate dehydrogenase of Bacillus stearothermophilus

Biochemistry ◽

10.1021/bi00013a011 ◽

1995 ◽

Vol 34 (13) ◽

pp. 4225-4230 ◽

Cited By ~ 9

Author(s):

James K. Hogan ◽

Carlos A. Pittol ◽

J. Bryan Jones ◽

Marvin Gold

Keyword(s):

Lactate Dehydrogenase ◽

Bacillus Stearothermophilus ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Side Chains ◽

Positively Charged

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Mapping motion in large proteins by single tryptophan probes inserted by site-directed mutagenesis: lactate dehydrogenase

Biochemical Society Transactions ◽

10.1042/bst0150991 ◽

1987 ◽

Vol 15 (5) ◽

pp. 991-993 ◽

Cited By ~ 13

Author(s):

TONY ATKINSON ◽

DAVID A. BARSTOW ◽

WILLIAM N. CHIA ◽

ANTHONY R. CLARKE ◽

KEITH W. HART ◽

...

Keyword(s):

Lactate Dehydrogenase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Large Proteins

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