Sequential Backbone Assignment in 13C-Labeled RNA via Through-Bond Coherence Transfer Using Three-Dimensional Triple Resonance Spectroscopy (1H,13C,31P) and Two-Dimensional Hetero TOCSY

1994 ◽  
Vol 116 (11) ◽  
pp. 4983-4984 ◽  
Author(s):  
H. A. Heus ◽  
S. S. Wijmenga ◽  
F. J. M. van de Ven ◽  
C. W. Hilbers
Keyword(s):  
Three Dimensional ◽  
Backbone Assignment ◽  
Resonance Spectroscopy ◽  
Triple Resonance ◽  
Two Dimensional ◽  
Coherence Transfer ◽  
Labeled Rna

Sequential backbone assignment of uniformly 13C-labeled RNAs by a two-dimensional P(CC)H-TOCSY triple resonance NMR experiment

1995 ◽  
Vol 5 (1) ◽  
pp. 82-86 ◽  
Author(s):  
S. S. Wijmenga ◽  
H. A. Heus ◽  
H. A. E. Leeuw ◽  
H. Hoppe ◽  
M. van der Graaf ◽  
...  
Keyword(s):  
Backbone Assignment ◽  
Triple Resonance ◽  
Triple Resonance Nmr ◽  
Two Dimensional

scholarly journals Novel Multidimensional Inverse Laplace Nuclear Magnetic Resonance Spectroscopy Techniques

2021 ◽  
Author(s):  
◽  
Kathryn Elizabeth Washburn
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Exchange Rate ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Mixing Time ◽  
Three Dimensional ◽  
Resonance Spectroscopy ◽  
Two Dimensional ◽  
Transverse Relaxation

<p>This thesis presents the new development and application of multidimensional inverse Laplace nuclear magnetic resonance spectroscopy techniques. We present a new NMR technique which relates the longitudinal relaxation rate of the NMR signal to the internal gradients in the sample. We perform the experiment on a large range of magnet strengths to provide experimental evidence for the theory of how internal gradient intensity scales with pore size as a function of field strength. We make the first attempt of quantisation of two dimensional inverse Laplace experiments. We perform a transverse relaxation exchange experiment on several samples for a range of mixing times. We then integrate the peaks in the resulting spectra and plot them as a function of mixing time. By fitting the experimental results to theory, we can estimate the molecular exchange between pores of differing sizes. We then modify the transverse relaxation experiment to include diffusion attenuation so that we can see the separate signals for oil and water. We use this to look at the effect wettability has on the movement of the different fluids between pores. We then present the first experiment to combine two inverse Laplace dimensions with a Fourier dimension. We add a propagator dimension to the transverse relaxation exchange experiment to measure how far the molecules move during the mixing time. Quantisation of the results allows us to estimate the exchange rate between pores of similar sizes in addition the exchange rate between pores of different sizes. We are also able to estimate pore radii, inter-pore spacing and tortuosity. Lastly, we attempt a three dimensional inverse Laplace experiment by correlating transverse relaxation, diffusion, and internal gradients. While the three dimensional inversion techniques require more development, the results show resemblance to those seen from two dimensional experiments.</p>

Two-dimensional triple-resonance HCNCH experiment for direct correlation of ribose H1' and base H8, H6 protons in 13C,15N-labeled RNA oligonucleotides

1993 ◽  
Vol 115 (25) ◽  
pp. 12181-12182 ◽  
Author(s):  
Vladimir Sklenar ◽  
Marita R. Rejante ◽  
Robert D. Peterson ◽  
Edmond Wang ◽  
Juli Feigon
Keyword(s):  
Triple Resonance ◽  
Two Dimensional ◽  
Labeled Rna
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