Pulsed EPR studies of mixed valent [Fe(II)Fe(III)] forms of hemerythrin and methane monooxygenase: evidence for a hydroxide bridge

1993 ◽  
Vol 115 (19) ◽  
pp. 8881-8882 ◽  
Author(s):  
H. Thomann ◽  
M. Bernardo ◽  
J. M. McCormick ◽  
S. Pulver ◽  
K. K. Andersson ◽  
...  
Keyword(s):  
Methane Monooxygenase ◽  
Pulsed Epr ◽  
Hydroxide Bridge

scholarly journals Probing the Structure of Membrane Proteins Using Pulsed EPR ESEEM Spectroscopy

2010 ◽  
Vol 98 (3) ◽  
pp. 232a
Author(s):  
You Zhou ◽  
Daniel J. Mayo ◽  
Robert M. McCarrick ◽  
Gary A. Lorigan
Keyword(s):  
Membrane Proteins ◽  
Pulsed Epr

scholarly journals Transient intermediates of the methane monooxygenase catalytic cycle.

1993 ◽  
Vol 268 (29) ◽  
pp. 21569-21577
Author(s):  
S.K. Lee ◽  
J.C. Nesheim ◽  
J.D. Lipscomb
Keyword(s):  
Methane Monooxygenase ◽  
Catalytic Cycle ◽  
Transient Intermediates

Soluble Methane Monooxygenase Component Interactions Monitored by 19F NMR

Biochemistry ◽  
2021 ◽  
Author(s):  
Jason C. Jones ◽  
Rahul Banerjee ◽  
Ke Shi ◽  
Manny M. Semonis ◽  
Hideki Aihara ◽  
...  
Keyword(s):  
Methane Monooxygenase ◽  
19F Nmr ◽  
Soluble Methane Monooxygenase

scholarly journals Methanobactin from Methylocystis sp. Strain SB2 Affects Gene Expression and Methane Monooxygenase Activity in Methylosinus trichosporium OB3b

2015 ◽  
Vol 81 (7) ◽  
pp. 2466-2473 ◽  
Author(s):  
Muhammad Farhan Ul-Haque ◽  
Bhagyalakshmi Kalidass ◽  
Alexey Vorobev ◽  
Bipin S. Baral ◽  
Alan A. DiSpirito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
Monooxygenase Activity ◽  
Content Type ◽  
Soluble Methane Monooxygenase ◽  
A Subunit ◽  
Membrane Bound

ABSTRACTMethanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced byMethylosinus trichosporiumOB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. WhenMethylosinus trichosporiumOB3b was grown in the presence of 1 μM CuCl2, expression ofmmoX, encoding a subunit of the hydroxylase component of sMMO, was very low.mmoXexpression increased, however, when methanobactin fromMethylocystissp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated withM. trichosporiumOB3b. IfM. trichosporiumOB3b was grown in the absence of CuCl2, themmoXexpression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure ofMethylosinus trichosporiumOB3b to SB2-Mb had no effect on expression ofmbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2.mbnAexpression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin “piracy” may be commonplace.

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