Solution conformation of a G.cntdot.TA [guanosine.cntdot.thymidine-5'-adenylic acid] triple in an intramolecular pyrimidine.cntdot.purine.cntdot.pyrimidine DNA triplex

Ishwar Radhakrishnan; Dinshaw J. Patel; James M. Veal; Xiaolian Gao

doi:10.1021/ja00043a045

Solution conformation of a G.cntdot.TA [guanosine.cntdot.thymidine-5'-adenylic acid] triple in an intramolecular pyrimidine.cntdot.purine.cntdot.pyrimidine DNA triplex

Journal of the American Chemical Society ◽

10.1021/ja00043a045 ◽

1992 ◽

Vol 114 (17) ◽

pp. 6913-6915 ◽

Cited By ~ 26

Author(s):

Ishwar Radhakrishnan ◽

Dinshaw J. Patel ◽

James M. Veal ◽

Xiaolian Gao

Keyword(s):

Solution Conformation ◽

Adenylic Acid ◽

Dna Triplex

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Solution Conformation of an Intramolecular DNA Triplex Containing a Nonnucleotide Linker: Comparison with the DNA Duplex†

Biochemistry ◽

10.1021/bi970710q ◽

1997 ◽

Vol 36 (47) ◽

pp. 14502-14511 ◽

Cited By ~ 21

Author(s):

John P. Bartley ◽

Tom Brown ◽

Andrew N. Lane

Keyword(s):

Dna Duplex ◽

Solution Conformation ◽

Dna Triplex

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Faculty Opinions recommendation of Solution conformation of Lys63-linked di-ubiquitin chain provides clues to functional diversity of polyubiquitin signaling.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1017888.206472 ◽

2004 ◽

Author(s):

Jane Endicott

Keyword(s):

Functional Diversity ◽

Solution Conformation ◽

Ubiquitin Chain

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Antigene and Antiproliferative Effects of Triplex-Forming Oligonucleotide (TFO) Targeted on hmgb1 Gene in Human Hepatoma Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200619170438 ◽

2020 ◽

Vol 20 (16) ◽

pp. 1943-1955

Author(s):

Neelam Lohani ◽

Moganty R. Rajeswari

Keyword(s):

Apoptotic Cell ◽

Isothermal Calorimetry ◽

High Mobility ◽

Rational Drug Design ◽

Human Hepatoma Cells ◽

Aberrant Expression ◽

Bioinformatic Tools ◽

Hmgb1 Expression ◽

Dna Triplex ◽

Triplex Forming Oligonucleotide

Background: The high mobility group box 1 (hmgb1) is one of the frequently over-expressed genes whose aberrant expression is reported in a number of human cancers. Various strategies are underway to inhibit hmgb1 expression in cancer cells having considerable therapeutic value. Objective: The present work involves selective transcriptional inhibition of the hmgb1 gene using selective DNA triplex structure-based gene technology. Here, the promoter region of the hmgb1 gene at position (-183 to -165) from the transcription start site as a target was selected using bioinformatic tools. Methods: The DNA triplex formation by the DNA of the target gene and TFO was confirmed using UV absorption spectroscopy, Circular Dichroism, and Isothermal Calorimetry. Results: Treatment of HepG2 cell with specific Triplex-forming Oligonucleotide significantly downregulated HMGB1 expression level at mRNA and protein levels by 50%, while the classical anticancer drugs, actinomycin/ adriamycin as positive controls showed 65% and the combination of TFO and drug decreased by 70%. The anti-proliferative effects of TFO correlated well with the fact of accumulation of cells in the Go phase and apoptotic cell death. Further, the binding of anti-cancer drugs to hmgb1 is stronger in DNA triplex state as compared to hmgb1 alone, suggesting the combination therapy as a better option. Conclusion: Therefore, the ability of hmgb1 targeted triplex-forming oligonucleotide in combination with triplex selective anticancer drug holds promise in the treatment of malignancies associated with hmgb1 overexpression. The result obtained may open up new vistas to provide a basis for the rational drug design and searching for high-affinity ligands with a high triplex selectivity.

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Reduction and tensammetric pulse-polarographic currents of polynucleotides

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19872810 ◽

1987 ◽

Vol 52 (11) ◽

pp. 2810-2818 ◽

Cited By ~ 11

Author(s):

Emil Paleček ◽

František Jelen ◽

Vladimír Vetterl

Keyword(s):

Diagnostic Criteria ◽

Pulse Width ◽

Mercury Electrode ◽

Pulse Amplitude ◽

Single Strand ◽

Pulse Polarography ◽

Dropping Mercury Electrode ◽

Adenylic Acid ◽

Drop Time

The behaviour of electrochemically reducible single-strand polynucleotides (poly(adenylic acid)) and poly(cytidylic acid)) was studied by the differential (derivative) pulse polarography (DPP) and by other methods. Measurements were performed with the help of the dropping mercury electrode under various conditions specified by the pulse width, pulse amplitude, drop time etc. For the faradaic and tensammetric DPP peaks the diagnostic criteria were proposed which make it possible to classify even very small DPP peaks of double helical polynucleotides.

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