Thiolation of uridine carbon-2 restricts the motional dynamics of the transfer RNA wobble position nucleoside

1992 ◽  
Vol 114 (7) ◽  
pp. 2652-2656 ◽  
Author(s):  
Paul F. Agris ◽  
Hanna Sierzputowska-Gracz ◽  
Wanda Smith ◽  
A. Malkiewicz ◽  
Elzbieta Sochacka ◽  
...  
Keyword(s):  
Transfer Rna ◽  
Wobble Position ◽  
Motional Dynamics

scholarly journals Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA

2019 ◽  
Vol 366 (10) ◽  
Author(s):  
Christopher Keller ◽  
Manas Chattopadhyay ◽  
Herbert Tabor
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Protein Biosynthesis ◽  
Transfer Rna ◽  
Coli Strain ◽  
E Coli ◽  
Wobble Position ◽  
Inhibition Of Growth ◽  
Absolute Requirement

Abstract The genes mnmE and mnmG are responsible for the modification of uridine 34, ‘the wobble position’ of many aminoacyl-tRNAs. Deletion of these genes affects the strength of the codon-anticodon interactions of the aminoacyl-tRNAs with the mRNAs and the ribosomes. However, deletion of these genes does not usually have a significant effect on the growth rate of the standard Escherichia coli strains. In contrast, we have found that if the host E. coli strain is deficient in the synthesis of polyamines, deletion of the mnmE or mnmG gene results in complete inhibition of growth unless the medium contains polyamines. The finding of an absolute requirement for polyamines in our current work will be significant in studies on polyamine function, in studies on the function of the mnmE/G genes, and in studies on the role of aminoacyl-tRNAs in protein biosynthesis.

ChemInform Abstract: Chemistry and Structure of Modified Uridines in the Anticodon, Wobble Position of Transfer RNA Are Determined by Thiolation

ChemInform ◽  
1988 ◽  
Vol 19 (10) ◽  
Author(s):  
H. SIERZPUTOWSKA-GRACZ ◽  
E. SOCHACKA ◽  
A. MALKIEWICZ ◽  
K. KUO ◽  
C. W. GEHRKE ◽  
...  
Keyword(s):  
Transfer Rna ◽  
Wobble Position

Chemistry and structure of modified uridines in the anticodon, wobble position of transfer RNA are determined by thiolation

1987 ◽  
Vol 109 (23) ◽  
pp. 7171-7177 ◽  
Author(s):  
Hanna Sierzputowska-Gracz ◽  
Elzbieta Sochacka ◽  
Andrzej Malkiewicz ◽  
Kenneth Kuo ◽  
Charles W. Gehrke ◽  
...  
Keyword(s):  
Transfer Rna ◽  
Wobble Position
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